MARCH9 (MARCHF9) (NM_138396) Human Tagged Lenti ORF Clone
CAT#: RC206159L1
- LentiORF®
Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 9 (MARCH9), Myc-DDK-tagged
"NM_138396" in other vectors (6)
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CNY 6,056.00
Cited in 1 publication. |
CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MARCH-IX; MARCH9; RNF179 |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC206159).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_138396 |
ORF Size | 1038 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138396.4, NP_612405.2 |
RefSeq Size | 2462 bp |
RefSeq ORF | 1041 bp |
Locus ID | 92979 |
UniProt ID | Q86YJ5 |
Protein Families | Transmembrane |
MW | 37.6 kDa |
Gene Summary | MARCH9 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH9 induces internalization of several membrane glycoproteins and directs them to the endosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Hoer et al., 2007 [PubMed 17174307]).[supplied by OMIM, Apr 2010] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A serine in the first transmembrane domain of the human E3 ubiquitin ligase MARCH9 is critical for down-regulation of its protein substrates
,null,
The Journal of Biological Chemistry
,PubMed ID 30554144
[MARCHF9]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206159 | MARCH9 (Myc-DDK-tagged)-Human membrane-associated ring finger (C3HC4) 9 (MARCH9) |
CNY 3,656.00 |
|
RC206159L2 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 9 (MARCH9), mGFP tagged |
CNY 5,890.00 |
|
RC206159L3 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 9 (MARCH9), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206159L4 | Lenti ORF clone of Human membrane-associated ring finger (C3HC4) 9 (MARCH9), mGFP tagged |
CNY 5,890.00 |
|
RG206159 | 41342 (tGFP-tagged) - Human membrane-associated ring finger (C3HC4) 9 (MARCH9) |
CNY 4,370.00 |
|
SC120445 | 41342 (untagged)-Human membrane-associated ring finger (C3HC4) 9 (MARCH9) |
CNY 3,656.00 |
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