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MAGEB18 (NM_173699) Human Tagged ORF Clone
CAT＃: RC206329
MAGEB18 (Myc-DDK-tagged)-Human melanoma antigen family B, 18 (MAGEB18)
ORF Plasmid: tGFP
"NM_173699" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206329 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGAGGTCAGAAGAGTAAGCTCCGTGCCCGTGAGAAACGCCACCAGGCTCGTTGTGAGAATCAGG ATCTGGGAGCTACGCAGGCCACTGTGGCAGAAGGAGAGTCACCCTCCTCTGCCTATCTTCTCTTTGGTGA CAGACCCCAGAATTTGCCTGCTGCTGAGACACCTAGCATCCCTGAAGCGCTTCAGGGAGCCCCATCCACC ACCAATGCTATTGCACCTGTTTCATGCAGTTCAAATGAAGGTGCCAGCAGCCAAGATGAGAAAAGTCTAG GTTCCTCAAGGGAAGCTGAGGGCTGGAAAGAAGATCCTTTAAACAAGAAAGTAGTGTCGCTGGTGCATTT CTTGCTTCAGAAGTATGAAACGAAAGAGCCAATTACAAAGGGAGATATGATAAAGTTTGTTATCAGGAAG GATAAGTGTCACTTCAATGAGATCCTCAAGAGAGCCTCTGAGCACATGGAGCTGGCACTTGGTGTTGATT TGAAGGAAGTGGATCCCATCAGGCACTACTATGCCTTTTTCAGCAAATTAGACCTCACCTATGATGAAAC AACCAGTGATGAAGAAAAAATTCCCAAGACTGGCCTCCTGATGATTGCACTGGGTGTGATCTTTCTGAAT GGCAACCGTGCCCCAGAAGAGGCAGTCTGGGAAATTATGAATATGATGGGTGTATATGCTGATAGGAAGC ACTTCCTCTATGGGGATCCCAGGAAGGTCATGACCAAAGATTTGGTGCAGCTAAAGTACCTGGAGTACCA GCAAGTGCCCAACAGTGATCCTCCACGCTATGAATTCCTGTGGGGTCCAAGAGCTCACGCTGAAACTAGC AAGATGAAAGTCCTGGAGTTTGTAGCCAAGATACATGATACCGTCCCTAGTGCCTTCCCATCCTGCTATG AAGAGGCTTTGAGGGATGAGGAACAGAGAACCCAAGCCAGAGCTGCAGCCAGGGCTCATACTGCTGCCAT GGCAAATGCACGTTCCAGAACCACGTCTAGCAGCTTCTCCCATGCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206329 protein sequence
Red=Cloning site Green=Tags(s) MPRGQKSKLRAREKRHQARCENQDLGATQATVAEGESPSSAYLLFGDRPQNLPAAETPSIPEALQGAPST TNAIAPVSCSSNEGASSQDEKSLGSSREAEGWKEDPLNKKVVSLVHFLLQKYETKEPITKGDMIKFVIRK DKCHFNEILKRASEHMELALGVDLKEVDPIRHYYAFFSKLDLTYDETTSDEEKIPKTGLLMIALGVIFLN GNRAPEEAVWEIMNMMGVYADRKHFLYGDPRKVMTKDLVQLKYLEYQQVPNSDPPRYEFLWGPRAHAETS KMKVLEFVAKIHDTVPSAFPSCYEEALRDEEQRTQARAAARAHTAAMANARSRTTSSSFSHAK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_173699 |
| ORF Size | 1029 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_173699.2 |
| RefSeq Size | 1812 bp |
| RefSeq ORF | 1032 bp |
| Locus ID | 286514 |
| UniProt ID | Q96M61 |
| MW | 38.5 kDa |
| Gene Summary | May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206329L3 | Lenti ORF clone of Human melanoma antigen family B, 18 (MAGEB18), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206329L4 | Lenti ORF clone of Human melanoma antigen family B, 18 (MAGEB18), mGFP tagged |
CNY 5,890.00 |
|
| RG206329 | MAGEB18 (tGFP-tagged) - Human melanoma antigen family B, 18 (MAGEB18) |
CNY 5,256.00 |
|
| SC123333 | MAGEB18 (untagged)-Human melanoma antigen family B, 18 (MAGEB18) |
CNY 3,656.00 |
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