United States
Germany
Japan
United Kingdom
China
FBXO28 (NM_015176) Human Tagged ORF Clone
CAT＃: RC206490
FBXO28 (Myc-DDK-tagged)-Human F-box protein 28 (FBXO28), transcript variant 1
ORF Plasmid: tGFP
"NM_015176" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CENP-30; Fbx28 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206490 representing NM_015176
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCAGCGGCGGAGGAGCGGATGGCAGAGGAAGGAGGCGGCGGCCAAGGCGACGGCGGTTCCTCTT TGGCCTCCGGCTCTACCCAGCGACAGCCTCCACCGCCCGCGCCACAGCACCCGCAGCCGGGGTCCCAGGC GCTCCCAGCCCCCGCGCTGGCTCCGGACCAGCTGCCTCAAAACAACACGCTTGTGGCGCTGCCCATCGTA GCCATCGAGAACATCCTCAGCTTTATGTCCTACGACGAAATTAGCCAGCTCCGCCTGGTTTGTAAAAGAA TGGACTTGGTCTGCCAGAGAATGTTGAATCAGGGATTTCTGAAAGTGGAGAGGTACCATAATCTATGTCA GAAACAAGTTAAAGCACAACTCCCAAGGAGAGAGTCAGAAAGGAGAAACCATTCATTAGCTCGTCATGCA GACATTCTTGCTGCTGTTGAAACAAGGCTGTCACTATTAAATATGACTTTCATGAAATATGTGGATTCCA ATCTCTGTTGCTTCATCCCAGGAAAGGTGATTGATGAGATTTATCGTGTGTTGAGATATGTCAATTCTAC CAGAGCCCCTCAACGAGCTCATGAAGTACTTCAAGAATTAAGGGATATATCCTCTATGGCAATGGAGTAC TTTGATGAAAAGATTGTTCCAATTTTAAAGAGGAAATTACCAGGATCAGATGTTTCTGGAAGACTCATGG GCTCTCCTCCAGTTCCAGGACCGTCTGCAGCCCTAACAACAATGCAGCTCTTCTCCAAGCAAAATCCTTC AAGACAAGAGGTTACCAAACTCCAGCAGCAGGTTAAAACAAATGGTGCTGGCGTGACTGTTCTCAGGCGT GAAATTTCTGAGCTTCGCACCAAAGTGCAAGAACAGCAAAAACAGCTTCAAGACCAGGACCAGAAACTGC TAGAGCAGACCCAGATCATAGGTGAACAAAATGCACGGTTGGCAGAGCTAGAACGCAAACTACGAGAAGT AATGGAAAGTGCTGTAGGAAATTCCTCAGGGTCCGGGCAGAATGAGGAGTCTCCTCGGAAACGAAAAAAG GCCACGGAAGCCATAGACTCTCTTAGGAAATCTAAACGTCTTCGGAATAGAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206490 representing NM_015176
Red=Cloning site Green=Tags(s) MAAAAEERMAEEGGGGQGDGGSSLASGSTQRQPPPPAPQHPQPGSQALPAPALAPDQLPQNNTLVALPIV AIENILSFMSYDEISQLRLVCKRMDLVCQRMLNQGFLKVERYHNLCQKQVKAQLPRRESERRNHSLARHA DILAAVETRLSLLNMTFMKYVDSNLCCFIPGKVIDEIYRVLRYVNSTRAPQRAHEVLQELRDISSMAMEY FDEKIVPILKRKLPGSDVSGRLMGSPPVPGPSAALTTMQLFSKQNPSRQEVTKLQQQVKTNGAGVTVLRR EISELRTKVQEQQKQLQDQDQKLLEQTQIIGEQNARLAELERKLREVMESAVGNSSGSGQNEESPRKRKK ATEAIDSLRKSKRLRNRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015176 |
| ORF Size | 1104 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015176.4 |
| RefSeq Size | 2923 bp |
| RefSeq ORF | 1107 bp |
| Locus ID | 23219 |
| UniProt ID | Q9NVF7 |
| Domains | F-box |
| Protein Families | Druggable Genome |
| MW | 41 kDa |
| Gene Summary | Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206490L1 | Lenti ORF clone of Human F-box protein 28 (FBXO28), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206490L2 | Lenti ORF clone of Human F-box protein 28 (FBXO28), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206490L3 | Lenti ORF clone of Human F-box protein 28 (FBXO28), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206490L4 | Lenti ORF clone of Human F-box protein 28 (FBXO28), transcript variant 1, mGFP tagged |
CNY 6,056.00 |
|
| RG206490 | FBXO28 (tGFP-tagged) - Human F-box protein 28 (FBXO28), transcript variant 1 |
CNY 5,256.00 |
|
| SC321801 | FBXO28 (untagged)-Human F-box protein 28 (FBXO28), transcript variant 1 |
CNY 3,656.00 |
