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CD162 (SELPLG) (NM_003006) Human Tagged ORF Clone
CAT＃: RC206507
SELPLG (Myc-DDK-tagged)-Human selectin P ligand (SELPLG), transcript variant 2
ORF Plasmid: tGFP
"NM_003006" in other vectors (7)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD162; CLA; PSGL-1; PSGL1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206507 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCTGCAACTCCTCCTGTTGCTGATCCTACTGGGCCCTGGCAACAGCTTGCAGCTGTGGGACACCT GGGCAGATGAAGCCGAGAAAGCCTTGGGTCCCCTGCTTGCCCGGGACCGGAGACAGGCCACCGAATATGA GTACCTAGATTATGATTTCCTGCCAGAAACGGAGCCTCCAGAAATGCTGAGGAACAGCACTGACACCACT CCTCTGACTGGGCCTGGAACCCCTGAGTCTACCACTGTGGAGCCTGCTGCAAGGCGTTCTACTGGCCTGG ATGCAGGAGGGGCAGTCACAGAGCTGACCACGGAGCTGGCCAACATGGGGAACCTGTCCACGGATTCAGC AGCTATGGAGATACAGACCACTCAACCAGCAGCCACGGAGGCACAGACCACTCCACTGGCAGCCACAGAG GCACAGACAACTCGACTGACGGCCACGGAGGCACAGACCACTCCACTGGCAGCCACAGAGGCACAGACCA CTCCACCAGCAGCCACGGAAGCACAGACCACTCAACCCACAGGCCTGGAGGCACAGACCACTGCACCAGC AGCCATGGAGGCACAGACCACTGCACCAGCAGCCATGGAAGCACAGACCACTCCACCAGCAGCCATGGAG GCACAGACCACTCAAACCACAGCCATGGAGGCACAGACCACTGCACCAGAAGCCACGGAGGCACAGACCA CTCAACCCACAGCCACGGAGGCACAGACCACTCCACTGGCAGCCATGGAGGCCCTGTCCACAGAACCCAG TGCCACAGAGGCCCTGTCCATGGAACCTACTACCAAAAGAGGTCTGTTCATACCCTTTTCTGTGTCCTCT GTTACTCACAAGGGCATTCCCATGGCAGCCAGCAATTTGTCCGTCAACTACCCAGTGGGGGCCCCAGACC ACATCTCTGTGAAGCAGTGCCTGCTGGCCATCCTAATCTTGGCGCTGGTGGCCACTATCTTCTTCGTGTG CACTGTGGTGCTGGCGGTCCGCCTCTCCCGCAAGGGCCACATGTACCCCGTGCGTAATTACTCCCCCACC GAGATGGTCTGCATCTCATCCCTGTTGCCTGATGGGGGTGAGGGGCCCTCTGCCACAGCCAATGGGGGCC TGTCCAAGGCCAAGAGCCCGGGCCTGACGCCAGAGCCCAGGGAGGACCGTGAGGGGGATGACCTCACCCT GCACAGCTTCCTCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206507 protein sequence
Red=Cloning site Green=Tags(s) MPLQLLLLLILLGPGNSLQLWDTWADEAEKALGPLLARDRRQATEYEYLDYDFLPETEPPEMLRNSTDTT PLTGPGTPESTTVEPAARRSTGLDAGGAVTELTTELANMGNLSTDSAAMEIQTTQPAATEAQTTPLAATE AQTTRLTATEAQTTPLAATEAQTTPPAATEAQTTQPTGLEAQTTAPAAMEAQTTAPAAMEAQTTPPAAME AQTTQTTAMEAQTTAPEATEAQTTQPTATEAQTTPLAAMEALSTEPSATEALSMEPTTKRGLFIPFSVSS VTHKGIPMAASNLSVNYPVGAPDHISVKQCLLAILILALVATIFFVCTVVLAVRLSRKGHMYPVRNYSPT EMVCISSLLPDGGEGPSATANGGLSKAKSPGLTPEPREDREGDDLTLHSFLP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003006 |
| ORF Size | 1206 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003006.3, NP_002997.1 |
| RefSeq Size | 2573 bp |
| RefSeq ORF | 1239 bp |
| Locus ID | 6404 |
| UniProt ID | Q14242 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs) |
| MW | 42.1 kDa |
| Gene Summary | This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Sulfatide decreases the resistance to stress-induced apoptosis and increases P-selectin-mediated adhesion: a two-edged sword in breast cancer progression
,Suchanski, J;Grzegrzolka, J;Owczarek, T;Pasikowski, P;Piotrowska, A;Kocbach, B;Nowak, A;Dziegiel, P;Wojnar, A;Ugorski, M;,
Breast Cancer Res.
,PubMed ID 30400820
[CD162]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206507L1 | Lenti ORF clone of Human selectin P ligand (SELPLG), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206507L2 | Lenti ORF clone of Human selectin P ligand (SELPLG), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC206507L3 | Lenti ORF clone of Human selectin P ligand (SELPLG), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206507L4 | Lenti ORF clone of Human selectin P ligand (SELPLG), transcript variant 2, mGFP tagged |
CNY 6,056.00 |
|
| RG206507 | SELPLG (tGFP-tagged) - Human selectin P ligand (SELPLG), transcript variant 2 |
CNY 5,256.00 |
|
| SC118237 | SELPLG (untagged)-Human selectin P ligand (SELPLG), transcript variant 2 |
CNY 3,656.00 |
|
| SC324184 | SELPLG (untagged)-Human selectin P ligand (SELPLG), transcript variant 2 |
CNY 3,656.00 |
