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Hepsin (HPN) (NM_002151) Human Tagged ORF Clone
CAT＃: RC206594
HPN (Myc-DDK-tagged)-Human hepsin (HPN), transcript variant 2
ORF Plasmid: tGFP
"NM_002151" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | TMPRSS1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206594 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGAAGGAGGGTGGCCGGACTGTGCCATGCTGCTCCAGACCCAAGGTGGCAGCTCTCACTGCGG GGACCCTGCTACTTCTGACAGCCATCGGGGCGGCATCCTGGGCCATTGTGGCTGTTCTCCTCAGGAGTGA CCAGGAGCCGCTGTACCCAGTGCAGGTCAGCTCTGCGGACGCTCGGCTCATGGTCTTTGACAAGACGGAA GGGACGTGGCGGCTGCTGTGCTCCTCGCGCTCCAACGCCAGGGTAGCCGGACTCAGCTGCGAGGAGATGG GCTTCCTCAGGGCACTGACCCACTCCGAGCTGGACGTGCGAACGGCGGGCGCCAATGGCACGTCGGGCTT CTTCTGTGTGGACGAGGGGAGGCTGCCCCACACCCAGAGGCTGCTGGAGGTCATCTCCGTGTGTGATTGC CCCAGAGGCCGTTTCTTGGCCGCCATCTGCCAAGACTGTGGCCGCAGGAAGCTGCCCGTGGACCGCATCG TGGGAGGCCGGGACACCAGCTTGGGCCGGTGGCCGTGGCAAGTCAGCCTTCGCTATGATGGAGCACACCT CTGTGGGGGATCCCTGCTCTCCGGGGACTGGGTGCTGACAGCCGCCCATTGCTTCCCGGAGCGGAACCGG GTCCTGTCCCGATGGCGAGTGTTTGCCGGTGCCGTGGCCCAGGCCTCTCCCCACGGTCTGCAGCTGGGGG TGCAGGCTGTGGTCTACCACGGGGGCTATCTTCCCTTTCGGGACCCCAACAGCGAGGAGAACAGCAACGA TATTGCCCTGGTCCACCTCTCCAGTCCCCTGCCCCTCACAGAATACATCCAGCCTGTGTGCCTCCCAGCT GCCGGCCAGGCCCTGGTGGATGGCAAGATCTGTACCGTGACGGGCTGGGGCAACACGCAGTACTATGGCC AACAGGCCGGGGTACTCCAGGAGGCTCGAGTCCCCATAATCAGCAATGATGTCTGCAATGGCGCTGACTT CTATGGAAACCAGATCAAGCCCAAGATGTTCTGTGCTGGCTACCCCGAGGGTGGCATTGATGCCTGCCAG GGCGACAGCGGTGGTCCCTTTGTGTGTGAGGACAGCATCTCTCGGACGCCACGTTGGCGGCTGTGTGGCA TTGTGAGTTGGGGCACTGGCTGTGCCCTGGCCCAGAAGCCAGGCGTCTACACCAAAGTCAGTGACTTCCG GGAGTGGATCTTCCAGGCCATAAAGACTCACTCCGAAGCCAGCGGCATGGTGACCCAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206594 protein sequence
Red=Cloning site Green=Tags(s) MAQKEGGRTVPCCSRPKVAALTAGTLLLLTAIGAASWAIVAVLLRSDQEPLYPVQVSSADARLMVFDKTE GTWRLLCSSRSNARVAGLSCEEMGFLRALTHSELDVRTAGANGTSGFFCVDEGRLPHTQRLLEVISVCDC PRGRFLAAICQDCGRRKLPVDRIVGGRDTSLGRWPWQVSLRYDGAHLCGGSLLSGDWVLTAAHCFPERNR VLSRWRVFAGAVAQASPHGLQLGVQAVVYHGGYLPFRDPNSEENSNDIALVHLSSPLPLTEYIQPVCLPA AGQALVDGKICTVTGWGNTQYYGQQAGVLQEARVPIISNDVCNGADFYGNQIKPKMFCAGYPEGGIDACQ GDSGGPFVCEDSISRTPRWRLCGIVSWGTGCALAQKPGVYTKVSDFREWIFQAIKTHSEASGMVTQL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002151 |
| ORF Size | 1251 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002151.2 |
| RefSeq Size | 1809 bp |
| RefSeq ORF | 1254 bp |
| Locus ID | 3249 |
| UniProt ID | P05981 |
| Domains | SR, Tryp_SPc |
| Protein Families | Druggable Genome, Protease, Transmembrane |
| MW | 45 kDa |
| Gene Summary | This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206594L1 | Lenti ORF clone of Human hepsin (HPN), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC206594L2 | Lenti ORF clone of Human hepsin (HPN), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC206594L3 | Lenti ORF clone of Human hepsin (HPN), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206594L4 | Lenti ORF clone of Human hepsin (HPN), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206594 | HPN (tGFP-tagged) - Human hepsin (HPN), transcript variant 2 |
CNY 5,256.00 |
|
| SC321709 | HPN (untagged)-Human hepsin (HPN), transcript variant 2 |
CNY 3,656.00 |
