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GCAP1 (GUCA1A) (NM_000409) Human Tagged ORF Clone
CAT＃: RC206595
GUCA1A (Myc-DDK-tagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A)
ORF Plasmid: tGFP
"NM_000409" in other vectors (7)
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CNY 2,400.00
CNY 3,705.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GUCA1A; GUCA1ANB-GUCA1A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206595 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAACGTGATGGAGGGAAAGTCAGTGGAGGAGCTGAGCAGCACCGAGTGCCACCAGTGGTACAAGA AGTTCATGACTGAGTGCCCCTCTGGCCAACTCACCCTCTATGAGTTCCGCCAGTTCTTCGGCCTCAAGAA CCTGAGCCCGTCGGCCAGCCAGTACGTGGAACAGATGTTTGAGACTTTTGACTTCAACAAGGACGGCTAC ATTGATTTCATGGAGTACGTGGCAGCGCTCAGCTTGGTCCTCAAGGGGAAGGTGGAACAGAAGCTCCGCT GGTACTTCAAGCTCTATGATGTAGATGGCAACGGCTGCATTGACCGCGATGAGCTGCTCACCATCATCCA GGCCATTCGCGCCATTAACCCCTGCAGCGATACCACCATGACTGCAGAGGAGTTCACCGATACAGTGTTC TCCAAGATTGACGTCAACGGGGATGGGGAACTCTCCCTGGAAGAGTTTATAGAGGGCGTCCAGAAGGACC AGATGCTCCTGGACACACTGACACGAAGCCTGGACCTTACCCGCATCGTGCGCAGGCTCCAGAATGGCGA GCAAGACGAGGAGGGGGCTGACGAGGCCGCTGAGGCAGCCGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206595 protein sequence
Red=Cloning site Green=Tags(s) MGNVMEGKSVEELSSTECHQWYKKFMTECPSGQLTLYEFRQFFGLKNLSPSASQYVEQMFETFDFNKDGY IDFMEYVAALSLVLKGKVEQKLRWYFKLYDVDGNGCIDRDELLTIIQAIRAINPCSDTTMTAEEFTDTVF SKIDVNGDGELSLEEFIEGVQKDQMLLDTLTRSLDLTRIVRRLQNGEQDEEGADEAAEAAG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000409 |
| ORF Size | 603 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000409.5 |
| RefSeq Size | 1918 bp |
| RefSeq ORF | 606 bp |
| Locus ID | 2978 |
| UniProt ID | P43080 |
| Domains | EFh |
| Protein Families | Druggable Genome |
| Protein Pathways | Olfactory transduction |
| MW | 22.9 kDa |
| Gene Summary | This gene encodes an enzyme that plays a role in the recovery of retinal photoreceptors from photobleaching. This enzyme promotes the activity of retinal guanylyl cyclase-1 (GC1) at low calcium concentrations and inhibits GC1 at high calcium concentrations. Mutations in this gene can cause cone dystrophy 3 and code-rod dystrophy 14. provided by RefSeq, Jul 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Investigation of quinoline-4-carboxylic acid as a highly potent scaffold for the development of alkaline phosphatase inhibitors: synthesis, SAR analysis and molecular modelling studies
,Iqbal, J;Khan, I;Shah, S;Ejaz, S;Ibrar, A;Hameed, S;,
RSC Advances
[GCAP1]
|
|
The progressive rod-cone degeneration (PRCD) protein is secreted through the conventional ER/Golgi-dependent pathway
,Remez, L;Zobor, D;Kohl, S;Ben-Yosef, T;,
Exp. Eye Res.
,PubMed ID 24992209
[GCAP1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206595L1 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206595L2 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), mGFP tagged |
CNY 5,890.00 |
|
| RC206595L3 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206595L4 | Lenti ORF clone of Human guanylate cyclase activator 1A (retina) (GUCA1A), mGFP tagged |
CNY 5,890.00 |
|
| RG206595 | GUCA1A (tGFP-tagged) - Human guanylate cyclase activator 1A (retina) (GUCA1A) |
CNY 4,000.00 |
|
| SC108438 | GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A) |
CNY 2,400.00 |
|
| SC324283 | GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A) |
CNY 2,400.00 |
