GCAP1 (GUCA1A) (NM_000409) Human Untagged Clone

CAT#: SC108438

GUCA1A (untagged)-Human guanylate cyclase activator 1A (retina) (GUCA1A)



  "NM_000409" in other vectors (7)

CNY 2,400.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-GUCA1A Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GUCA1A; GUCA1ANB-GUCA1A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_000409, the custom clone sequence may differ by one or more nucleotides


ATGGGCAACGTGATGGAGGGAAAGTCAGTGGAGGAGCTGAGCAGCACCGAGTGCCACCAGTGGTACAAGA
AGTTCATGACTGAGTGCCCCTCTGGCCAACTCACCCTCTATGAGTTCCGCCAGTTCTTCGGCCTCAAGAA
CCTGAGCCCGTCGGCCAGCCAGTACGTGGAACAGATGTTTGAGACTTTTGACTTCAACAAGGACGGCTAC
ATTGATTTCATGGAGTACGTGGCAGCGCTCAGCTTGGTCCTCAAGGGGAAGGTGGAACAGAAGCTCCGCT
GGTACTTCAAGCTCTATGATGTAGATGGCAACGGCTGCATTGACCGCGATGAGCTGCTCACCATCATCCA
GGCCATTCGCGCCATTAACCCCTGCAGCGATACCACCATGACTGCAGAGGAGTTCACCGATACAGTGTTC
TCCAAGATTGACGTCAACGGGGATGGGGAACTCTCCCTGGAAGAGTTTATAGAGGGCGTCCAGAAGGACC
AGATGCTCCTGGACACACTGACACGAAGCCTGGACCTTACCCGCATCGTGCGCAGGCTCCAGAATGGCGA
GCAAGACGAGGAGGGGGCTGACGAGGCCGCTGAGGCAGCCGGCTGA


>OriGene 5' read for NM_000409 unedited
ATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCAGGCAGCGCT
CGCTAAAACCCANATGCCTGTGAACAGACAGCGCCAGCCATTCACACCCCAGTGGATGCT
GGCTTATTAGATTTGATTGGCAGCCTCTGGAGTAGGCAGGGTGGGCTATACAGGGCGTCT
AGGAAGACAGATAGGTCACGGCGGAGACAGGGCTGGCCCCCTCGCTGCATCTCCGAGGGT
CCGGGTCTCCCCTCAGCGTCTCTTGGGGCTTGCCGAGATGGGCGGAGCCTTGGGTTATGA
TGGGCGGGGCCTGAGGCTGGAGTGAGCGGGGCCCGGATGGGCTCACGGCGGCCGCGCCCC
TCGCCCAGGACGGCTACATTGATTTCATGGAGTACGTGGCAGCGCTCAGCTTGGTCCTCA
AGGGGAAGGTGGAACAGAAGCTCCGCTGGTACTTCAAGCTCTATGATGTAGATGGCAACG
GCTGCATTGACCGCGATGAGCTGCTCACCATCATCCAGGCCATTCGCGCCATTAACCCCT
GCAGCGATACCACCATGACTGCAGAGGAGTTCACCGATACAGTGTTCTCCAAGATTGACG
TCAACGGGGATGGGGAACTCTCCCTGGAAGAGTTTATAGAGGGCGTNCAGAAGGACCAGA
TGCTCCTGGACACACTGACACGAAGCCTGGACCTTACCCGCATCGTGCGCAGGCTCCAGA
ATGGCGAGCAAGACGAGGAGGGGGCTGACGAGGCCGCTNGAGCAGCCGGCTGAGTGCACC
GCCCGGCTGCTTCTCACTAGCGGTGGGGTGGTATNGGGGGGGGCTGTGGGGGGTGTTCTT
TGCCTTACCCTAGATAGATCTATGAC
Restriction Sites NotI-NotI     
ACCN NM_000409
Insert Size 2250 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000409.2, NP_000400.2
RefSeq Size 1976 bp
RefSeq ORF 606 bp
Locus ID 2978
UniProt ID P43080
Domains EFh
Protein Families Druggable Genome
Protein Pathways Olfactory transduction
Gene Summary This gene encodes an enzyme that plays a role in the recovery of retinal photoreceptors from photobleaching. This enzyme promotes the activity of retinal guanylyl cyclase-1 (GC1) at low calcium concentrations and inhibits GC1 at high calcium concentrations. Mutations in this gene can cause cone dystrophy 3 and code-rod dystrophy 14. provided by RefSeq, Jul 2020]
Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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