LECT1 (CNMD) (NM_001011705) Human Tagged ORF Clone
CAT#: RC206635
- TrueORF®
LECT1 (Myc-DDK-tagged)-Human leukocyte cell derived chemotaxin 1 (LECT1), transcript variant 2
ORF Plasmid: tGFP
"NM_001011705" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | BRICD3; CHM-I; CHM1; LECT1; MYETS1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC206635 representing NM_001011705
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAGAGAACTCCGACAAAGTTCCCATTGCCCTGGTGGGACCTGATGACGTGGAATTCTGCAGCCCCC CGGCGTACGCTACGCTGACGGTGAAGCCCTCCAGCCCCGCGCGGCTGCTCAAGGTGGGAGCCGTGGTCCT CATTTCGGGAGCTGTGCTGCTGCTCTTTGGGGCCATCGGGGCCTTCTACTTCTGGAAGGGGAGCGACAGT CACATTTACAATGTCCATTACACCATGAGTATCAATGGGAAATTACAAGATGGGTCAATGGAAATAGACG CTGGGAACAACTTGGAGACCTTTAAAATGGGAAGTGGAGCTGAAGAAGCAATTGCAGTTAATGATTTCCA GAATGGCATCACAGGAATTCGTTTTGCTGGAGGAGAGAAGTGCTACATTAAAGCGCAAGTGAAGGCTCGT ATTCCTGAGGTGGGCGCCGTGACCAAACAGAGCATCTCCTCCAAACTGGAAGGCAAGATCATGCCAGTCA AATATGAAGAAAATTCTCTTATCTGGGTGGCTGTAGATCAGCCTGTGAAGGACAACAGCTTCTTGAGTTC TAAGGTGTTAGAACTCTGCGGTGACCTTCCTATTTTCTGGCTTAAACCAACCTATCCAAAAGAAATCCAG AGGGAAAGAAGAGAAGTGGTAAGAAAAATTGTTCCAACTACCACAAAAAGACCACACAGTGGACCACGGA GCAACCCAGGCGCTGGAAGACTGAATAATGAAACCAGACCCAGTGTTCAAGAGGACTCACAAGCCTTCAA TCCTGATAATCCTTATCATCAGGAAGGGGAAAGCATGACATTCGACCCTAGACTGGATCACGAAGGAATC TGTTGTATAGAATGTAGGCGGAGCTACACCCACTGCCAGAAGATCTGTGAACCCCTGGGGGGCTATTACC CATGGCCTTATAATTATCAAGGCTGCCGTTCGGCCTGCAGAGTCATCATGCCATGTAGCTGGTGGGTGGC CCGTATCTTGGGCATGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206635 representing NM_001011705
Red=Cloning site Green=Tags(s) MTENSDKVPIALVGPDDVEFCSPPAYATLTVKPSSPARLLKVGAVVLISGAVLLLFGAIGAFYFWKGSDS HIYNVHYTMSINGKLQDGSMEIDAGNNLETFKMGSGAEEAIAVNDFQNGITGIRFAGGEKCYIKAQVKAR IPEVGAVTKQSISSKLEGKIMPVKYEENSLIWVAVDQPVKDNSFLSSKVLELCGDLPIFWLKPTYPKEIQ RERREVVRKIVPTTTKRPHSGPRSNPGAGRLNNETRPSVQEDSQAFNPDNPYHQEGESMTFDPRLDHEGI CCIECRRSYTHCQKICEPLGGYYPWPYNYQGCRSACRVIMPCSWWVARILGMV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001011705 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001011705.2 |
RefSeq Size | 1535 bp |
RefSeq ORF | 1002 bp |
Locus ID | 11061 |
UniProt ID | O75829 |
Protein Families | Secreted Protein, Transmembrane |
MW | 36.97 kDa |
Gene Summary | This gene encodes a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. This gene is expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone. It may be involved also in the broad control of tissue vascularization during development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206635L3 | Lenti ORF clone of Human leukocyte cell derived chemotaxin 1 (LECT1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206635L4 | Lenti ORF clone of Human leukocyte cell derived chemotaxin 1 (LECT1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG206635 | LECT1 (tGFP-tagged) - Human leukocyte cell derived chemotaxin 1 (LECT1), transcript variant 2 |
CNY 4,370.00 |
|
SC301600 | LECT1 (untagged)-Human leukocyte cell derived chemotaxin 1 (LECT1), transcript variant 2 |
CNY 3,656.00 |