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MED19 (NM_153450) Human Tagged ORF Clone
CAT＃: RC206806
MED19 (Myc-DDK-tagged)-Human mediator complex subunit 19 (MED19)
ORF Plasmid: tGFP
"NM_153450" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DT2P1G7; LCMR1; MED19AS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206806 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAATTTCACGGCACTGTTTGGGGCTCAGGCTGACCCACCACCGCCCCCAACCGCACTCGGCTTCG GACCAGGAAAGCCTCCACCCCCGCCACCGCCTCCTGCGGGCGGGGGACCCGGCACGGCCCCGCCTCCCAC CGCGGCCACGGCTCCTCCTGGCGCGGACAAGTCAGGAGCTGGCTGTGGCCCTTTTTACCTCATGAGGGAA CTGCCAGGTAGCACAGAGCTGACAGGCAGCACGAATCTGATCACACACTACAACTTGGAACAAGCCTATA ATAAATTCTGTGGGAAGAAGGTGAAGGAGAAGCTAAGTAACTTCCTGCCTGACCTGCCAGGGATGATTGA TCTGCCTGGTTCCCATGATAACAGCAGCCTCCGCTCTCTCATTGAGAAGCCCCCTATTCTCAGTAGCTCT TTCAATCCTATCACAGGGACCATGCTGGCCGGCTTCCGCCTCCACACTGGCCCGTTGCCGGAGCAGTGTC GTCTGATGCATATTCAGCCTCCCAAGAAGAAGAATAAGCACAAGCACAAACAGAGCCGTACCCAGGATCC TGTCCCCCCAGGTAAACCCAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206806 protein sequence
Red=Cloning site Green=Tags(s) MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRE LPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSS FNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153450 |
| ORF Size | 582 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153450.4 |
| RefSeq Size | 1605 bp |
| RefSeq ORF | 585 bp |
| Locus ID | 219541 |
| UniProt ID | A0JLT2 |
| Protein Families | Druggable Genome |
| MW | 20.4 kDa |
| Gene Summary | The protein encoded by this gene is a subunit of the Mediator complex, which binds to gene-specific regulatory factors and provides support for the basal RNA polymerase II transcription machinery. This gene has been implicated in the growth of several types of cancer, and inhibition of its expression inhibits the growth and spread of these cancers. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206806L1 | Lenti ORF clone of Human mediator complex subunit 19 (MED19), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC206806L2 | Lenti ORF clone of Human mediator complex subunit 19 (MED19), mGFP tagged |
CNY 5,890.00 |
|
| RC206806L3 | Lenti ORF clone of Human mediator complex subunit 19 (MED19), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206806L4 | Lenti ORF clone of Human mediator complex subunit 19 (MED19), mGFP tagged |
CNY 5,890.00 |
|
| RG206806 | MED19 (tGFP-tagged) - Human mediator complex subunit 19 (MED19) |
CNY 4,000.00 |
|
| SC123306 | MED19 (untagged)-Human mediator complex subunit 19 (MED19) |
CNY 2,400.00 |
