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Cullin 4B (CUL4B) (NM_003588) Human Tagged ORF Clone
CAT＃: RC206935
CUL4B (Myc-DDK-tagged)-Human cullin 4B (CUL4B), transcript variant 1
ORF Plasmid: tGFP
"NM_003588" in other vectors (6)
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CNY 6,688.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CUL-4B; MRXHF2; MRXS15; MRXSC; SFM2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206935 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTCACAGTCATCTGGATCAGGAGATGGGAATGATGATGAGGCTACTACCTCTAAAGACGGTGGTT TTTCTTCCCCCAGTCCCTCAGCTGCTGCTGCTGCTCAGGAGGTCAGATCTGCCACTGATGGTAATACCAG CACCACTCCGCCCACCTCTGCCAAGAAGAGAAAGTTAAACAGCAGCAGCAGTAGCAGCAGTAACAGTAGT AACGAGAGAGAAGACTTTGATTCCACCTCTTCCTCCTCTTCCACTCCTCCTTTACAACCCAGGGATTCGG CATCCCCTTCAACCTCGTCCTTCTGCCTGGGGGTTTCAGTGGCTGCTTCCAGCCACGTACCGATACAGAA GAAGCTGCGTTTTGAAGACACCCTGGAGTTTGTAGGGTTTGATGCGAAGATGGCTGAGGAATCCTCCTCC TCCTCCTCCTCATCTTCACCAACTGCTGCAACATCTCAGCAGCAGCAACTTAAAAATAAGAGTATATTAA TCTCTTCTGTGGCTTCGGTGCATCATGCAAACGGCCTAGCCAAATCTTCTACCACCGTCTCTAGCTTTGC TAACAGCAAACCTGGCTCTGCTAAGAAGTTAGTGATCAAGAACTTTAAAGATAAGCCTAAATTACCAGAA AACTACACAGATGAAACCTGGCAAAAACTGAAAGAAGCAGTGGAAGCTATTCAGAATAGTACTTCAATTA AGTACAATTTAGAAGAACTCTACCAGGCTGTAGAAAATCTCTGTTCTTACAAGATTTCTGCAAACTTGTA CAAACAGCTGAGACAGATCTGCGAAGATCACATCAAAGCACAGATTCATCAATTCAGAGAGGATTCATTG GATAGCGTTCTTTTTTTAAAGAAGATTGATAGATGCTGGCAAAACCATTGCAGACAAATGATCATGATCA GGAGCATTTTTTTGTTTCTGGATAGAACTTACGTTCTTCAGAATTCAATGCTACCCTCCATTTGGGACAT GGGACTGGAGTTATTTAGGGCTCATATTATAAGTGATCAGAAAGTGCAGAATAAGACAATTGATGGCATT CTTCTCTTGATTGAGAGGGAAAGGAATGGTGAAGCAATTGATAGAAGTTTACTTCGAAGCCTTTTAAGCA TGCTGTCTGATTTGCAAATTTATCAAGATTCTTTTGAACAACGATTTTTGGAAGAAACTAACCGGCTCTA TGCAGCTGAAGGCCAAAAATTAATGCAAGAAAGAGAGGTTCCTGAATATCTACATCATGTTAACAAACGT CTAGAAGAAGAAGCAGACAGACTTATTACTTACTTAGATCAGACCACCCAGAAGTCATTAATTGCTACTG TAGAAAAACAACTTCTAGGTGAACACTTAACAGCAATTCTTCAGAAAGGTTTAAATAACCTCCTTGATGA AAACCGAATTCAAGATTTGTCTCTTCTGTATCAGCTCTTCAGTAGAGTTCGAGGTGGAGTTCAGGTTCTT TTGCAGCAGTGGATCGAATATATCAAGGCATTTGGCAGCACTATTGTAATTAATCCTGAAAAAGATAAAA CCATGGTTCAAGAATTGCTGGATTTTAAAGATAAGGTTGACCATATAATTGATATCTGCTTTCTGAAGAA TGAGAAATTTATCAATGCCATGAAAGAAGCATTTGAAACGTTCATTAACAAAAGACCAAATAAACCAGCT GAACTTATAGCTAAGTATGTAGATTCAAAACTTCGTGCAGGCAACAAAGAAGCTACAGATGAAGAACTTG AGAAAATGTTGGATAAAATTATGATCATATTTAGATTTATCTATGGCAAGGATGTTTTTGAGGCCTTCTA TAAGAAAGATTTAGCCAAGCGCCTGTTAGTCGGAAAGAGTGCATCTGTAGATGCTGAAAAATCAATGCTG TCCAAACTTAAACATGAATGCGGAGCTGCTTTCACCAGCAAACTTGAAGGAATGTTTAAAGACATGGAAC TTTCTAAAGACATCATGATTCAGTTCAAACAGTATATGCAGAATCAGAATGTTCCGGGAAATATTGAGTT AACTGTGAATATCCTGACAATGGGCTATTGGCCGACATATGTGCCTATGGAAGTTCATTTACCACCAGAG ATGGTAAAACTTCAGGAGATTTTCAAGACATTTTACCTAGGCAAACATAGTGGCAGGAAACTTCAGTGGC AGTCAACCATAGGACACTGTGTGTTAAAAGCAGAATTTAAAGAGGGTAAAAAGGAACTCCAGGTCTCTCT TTTTCAAACACTGGTGCTGCTAATGTTTAATGAGGGAGAGGAGTTCAGTTTAGAAGAGATCAAGCAGGCA ACTGGAATAGAGGATGGAGAGTTAAGGAGAACACTGCAGTCATTAGCCTGTGGCAAAGCTAGAGTTCTGG CGAAAAATCCAAAGGGCAAAGACATTGAAGATGGTGACAAGTTCATTTGTAATGATGATTTCAAACATAA ACTTTTCAGGATAAAGATCAATCAAATCCAGATGAAAGAAACGGTTGAAGAACAAGCAAGCACTACAGAA AGAGTATTTCAAGACAGACAGTATCAAATTGATGCTGCAATTGTTCGAATTATGAAGATGAGAAAGACAC TTAGCCACAATCTCCTTGTTTCAGAAGTGTACAACCAGTTGAAATTTCCAGTAAAGCCTGCTGATCTTAA GAAGAGAATAGAATCTTTAATTGACCGGGACTACATGGAAAGAGATAAAGAAAATCCAAACCAGTACAAC TATATTGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206935 protein sequence
Red=Cloning site Green=Tags(s) MMSQSSGSGDGNDDEATTSKDGGFSSPSPSAAAAAQEVRSATDGNTSTTPPTSAKKRKLNSSSSSSSNSS NEREDFDSTSSSSSTPPLQPRDSASPSTSSFCLGVSVAASSHVPIQKKLRFEDTLEFVGFDAKMAEESSS SSSSSSPTAATSQQQQLKNKSILISSVASVHHANGLAKSSTTVSSFANSKPGSAKKLVIKNFKDKPKLPE NYTDETWQKLKEAVEAIQNSTSIKYNLEELYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSL DSVLFLKKIDRCWQNHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGI LLLIERERNGEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNKR LEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQLFSRVRGGVQVL LQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEKFINAMKEAFETFINKRPNKPA ELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYGKDVFEAFYKKDLAKRLLVGKSASVDAEKSML SKLKHECGAAFTSKLEGMFKDMELSKDIMIQFKQYMQNQNVPGNIELTVNILTMGYWPTYVPMEVHLPPE MVKLQEIFKTFYLGKHSGRKLQWQSTIGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQA TGIEDGELRRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQASTTE RVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQLKFPVKPADLKKRIESLIDRDYMERDKENPNQYN YIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003588 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003588.2, NP_003579.2 |
| RefSeq Size | 5365 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 8450 |
| UniProt ID | Q13620 |
| Domains | CULLIN |
| Protein Pathways | Nucleotide excision repair, Ubiquitin mediated proteolysis |
| MW | 104 kDa |
| Gene Summary | This gene is a member of the cullin family. The encoded protein forms a complex that functions as an E3 ubiquitin ligase and catalyzes the polyubiquitination of specific protein substrates in the cell. The protein interacts with a ring finger protein, and is required for the proteolysis of several regulators of DNA replication including chromatin licensing and DNA replication factor 1 and cyclin E. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation
,Jang, SM;Nathans, JF;Fu, H;Redon, CE;Jenkins, LM;Thakur, BL;Pongor, LS;Baris, AM;Gross, JM;OʹNeill, MJ;Indig, FE;Cappell, SD;Aladjem, MI;,
Nat Commun
,PubMed ID 31911655
[Cullin 4B]
|
|
Proteomic analysis of the Cullin4B interactome using proximity-dependent biotinylation in living cells
,Zhang, H;Li, S;Liu, P;Lee, FH;Wong, AH;Liu, F;,
Proteomics
,PubMed ID 28225217
[Cullin 4B]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206935L1 | Lenti ORF clone of Human cullin 4B (CUL4B), transcript variant 1, Myc-DDK-tagged |
CNY 9,088.00 |
|
| RC206935L2 | Lenti ORF clone of Human cullin 4B (CUL4B), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC206935L3 | Lenti ORF clone of Human cullin 4B (CUL4B), transcript variant 1, Myc-DDK-tagged |
CNY 9,088.00 |
|
| RC206935L4 | Lenti ORF clone of Human cullin 4B (CUL4B), transcript variant 1, mGFP tagged |
CNY 9,088.00 |
|
| RG206935 | CUL4B (tGFP-tagged) - Human cullin 4B (CUL4B), transcript variant 1 |
CNY 8,288.00 |
|
| SC128242 | CUL4B (untagged)-Human cullin 4B (CUL4B), transcript variant 1 |
CNY 6,696.00 |
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