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SPO11 (NM_198265) Human Tagged ORF Clone
CAT＃: RC206998
SPO11 (Myc-DDK-tagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2
ORF Plasmid: tGFP
"NM_198265" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CT35; SPATA43; TOPOVIA; TOPVIA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC206998 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTTTGCACCTATGGGGCCCGAGGCCTCGTTCTTCGACGTTTTGGACCGACACAGGGAGTCCCTGC TGGCTGCCCTGAGGAGAGGTGGCAGGGAGCCCCCAACTGGGGGAAGCCGCCTGGCCTCCAGGTTTGAAGA TTCTGTGGGTCTTCAGATGGTATCCCATTGCACCACCAGAAAGATCAAAAGTGATTCACCAAAATCAGCT CAAAAATTTTCTCTAATCCTTAAAATATTGTCCATGATTTATAAATTAGTACAGAGCAACACTTATGCAA CCAAAAGGGACATATATTACACTGACAGTCAACTCTTTGGTAACCAGACTGTCGTCGACAATATTATCAA TGACATTTCTTGCATGTTAAAAGTGTCAAGGAGGAGTCTACATATATTATCTACATCAAAAGGTTTAATT GCTGGCAACTTAAGATACATCGAGGAAGATGGCACCAAAGTGAATTGTACCTGTGGTGCAACGGCTGTTG CTGTGCCATCGAATATTCAAGGAATTCGGAATTTAGTTACAGATGCAAAGTTTGTATTAATTGTAGAAAA AGATGCAACATTTCAGCGGCTCCTAGATGACAACTTTTGCAACAAATTGTCTCCTTGCATCATGATTACG GGAAAGGGAGTTCCTGATCTAAACACAAGACTTTTAGTCAAGAAACTGTGGGATACATTTCATGTTCCTG TTTTCACTCTTGTAGATGCTGATCCACATGGCATAGAAATAATGTGCATCTATAAGTATGGATCTATGTC TATGTCTTTTGAAGCTCATCATCTCACAGTTCCAGCTATTAGATGGCTTGGTCTTCTCCCTTCTGATCTT AAAAGATTAAATGTACCTAAAGATAGTTTGATTCCACTGACAAAAAGGGACCAAATGAAACTTGACAGTA TCCTGAGGAGACCTTATGTTACCTGCCAACCATTTTGGAGAAAAGAAATGGAAATAATGGCAGACTCTAA AATGAAGGCAGAAATTCAAGCTTTGACTTTCCTATCATCAGATTATCTTTCCAGAGTGTACTTACCTAAC AAATTAAAATTTGGAGGATGGATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC206998 protein sequence
Red=Cloning site Green=Tags(s) MAFAPMGPEASFFDVLDRHRESLLAALRRGGREPPTGGSRLASRFEDSVGLQMVSHCTTRKIKSDSPKSA QKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDISCMLKVSRRSLHILSTSKGLI AGNLRYIEEDGTKVNCTCGATAVAVPSNIQGIRNLVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMIT GKGVPDLNTRLLVKKLWDTFHVPVFTLVDADPHGIEIMCIYKYGSMSMSFEAHHLTVPAIRWLGLLPSDL KRLNVPKDSLIPLTKRDQMKLDSILRRPYVTCQPFWRKEMEIMADSKMKAEIQALTFLSSDYLSRVYLPN KLKFGGWI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198265 |
| ORF Size | 1074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198265.2 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 23626 |
| UniProt ID | Q9Y5K1 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 40.4 kDa |
| Gene Summary | Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206998L3 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206998L4 | Lenti ORF clone of Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG206998 | SPO11 (tGFP-tagged) - Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 |
CNY 4,370.00 |
|
| SC125697 | SPO11 (untagged)-Human SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae) (SPO11), transcript variant 2 |
CNY 3,656.00 |
