Neuroglobin (NGB) (NM_021257) Human Tagged ORF Clone
CAT#: RC207482
NGB (Myc-DDK-tagged)-Human neuroglobin (NGB)
ORF Plasmid: tGFP
"NM_021257" in other vectors (6)
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CNY 1,200.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207482 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCGCCCGGAGCCCGAGCTGATCCGGCAGAGCTGGCGGGCAGTGAGCCGCAGCCCGCTGGAGCACG GCACCGTCCTGTTTGCCAGGCTGTTTGCCCTGGAGCCTGACCTGCTGCCCCTCTTCCAGTACAACTGCCG CCAGTTCTCCAGCCCAGAGGACTGTCTCTCCTCGCCTGAGTTCCTGGACCACATCAGGAAGGTGATGCTC GTGATTGATGCTGCAGTGACCAATGTGGAAGACCTGTCCTCACTGGAGGAGTACCTTGCCAGCCTGGGCA GGAAGCACCGGGCAGTGGGTGTGAAGCTCAGCTCCTTCTCGACAGTGGGTGAGTCTCTGCTCTACATGCT GGAGAAGTGTCTGGGCCCTGCCTTCACACCAGCCACACGGGCTGCCTGGAGCCAACTCTACGGGGCCGTA GTGCAGGCCATGAGTCGAGGCTGGGATGGCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207482 protein sequence
Red=Cloning site Green=Tags(s) MERPEPELIRQSWRAVSRSPLEHGTVLFARLFALEPDLLPLFQYNCRQFSSPEDCLSSPEFLDHIRKVML VIDAAVTNVEDLSSLEEYLASLGRKHRAVGVKLSSFSTVGESLLYMLEKCLGPAFTPATRAAWSQLYGAV VQAMSRGWDGE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_021257 |
ORF Size | 453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021257.3, NP_067080.1 |
RefSeq Size | 1885 bp |
RefSeq ORF | 456 bp |
Locus ID | 58157 |
UniProt ID | Q9NPG2 |
MW | 16.9 kDa |
Gene Summary | This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
---|
Neuroglobin overexpression plays a pivotal role in neuroprotection through mitochondrial raft-like microdomains in neuroblastoma SK-N-BE2 cells.
,null,
Molecular and cellular neurosciences
,PubMed ID 29378245
[Neuroglobin]
|
Hemin protects against oxygen-glucose deprivation-induced apoptosis activation via neuroglobin in SH-SY5Y cells
,Wang, YJ;Peng, QY;Deng, SY;Chen, CX;Wu, L;Huang, L;Zhang, LN;,
Neurochem. Res
,PubMed ID 28316021
[Neuroglobin]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207482L1 | Lenti ORF clone of Human neuroglobin (NGB), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC207482L2 | Lenti ORF clone of Human neuroglobin (NGB), mGFP tagged |
CNY 5,890.00 |
|
RC207482L3 | Lenti ORF clone of Human neuroglobin (NGB), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC207482L4 | Lenti ORF clone of Human neuroglobin (NGB), mGFP tagged |
CNY 3,600.00 |
|
RG207482 | NGB (tGFP-tagged) - Human neuroglobin (NGB) |
CNY 2,800.00 |
|
SC122910 | NGB (untagged)-Human neuroglobin (NGB) |
CNY 1,200.00 |
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