United States
Germany
Japan
United Kingdom
China
RASA3 (NM_007368) Human Tagged ORF Clone
CAT＃: RC207848
RASA3 (Myc-DDK-tagged)-Human RAS p21 protein activator 3 (RASA3)
ORF Plasmid: tGFP
"NM_007368" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,112.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GAP1IP4BP; GAPIII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207848 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTGGAGGACGAGGGGCTCCGGGTCTTCCAGAGCGTGAAGATCAAGATCGGTGAAGCCAAAAACC TTCCCTCTTACCCGGGGCCGAGCAAGATGAGGGATTGCTACTGCACGGTGAACCTGGACCAGGAGGAGGT TTTCAGGACCAAAATTGTGGAAAAGTCACTCTGCCCGTTTTACGGAGAAGACTTTTACTGTGAAATTCCT CGGAGCTTTCGTCACCTGTCCTTCTACATTTTCGATAGAGACGTTTTCCGGAGGGATTCCATCATAGGGA AGGTGGCCATCCAGAAGGAGGACTTGCAGAAGTACCACAACAGGGACACCTGGTTCCAGCTGCAGCACGT GGACGCTGACTCGGAAGTGCAGGGCAAAGTGCACCTGGAGCTGCGGCTGAGCGAGGTCATCACAGACACT GGGGTCGTCTGCCACAAGCTCGCCACACGCATCGTCGAGTGCCAGGGCCTCCCCATCGTGAATGGGCAAT GTGACCCCTACGCCACCGTGACGCTGGCAGGACCCTTCAGATCAGAAGCAAAGAAGACGAAAGTGAAGAG GAAGACCAACAATCCCCAGTTCGATGAAGTGTTTTATTTTGAGGTGACCCGGCCCTGTAGCTACAGCAAG AAGTCCCACTTTGACTTTGAGGAGGAAGACGTGGACAAGCTCGAAATCAGAGTTGACCTCTGGAATGCCA GTAACCTGAAGTTTGGAGATGAATTCCTGGGAGAACTAAGGATCCCGTTGAAAGTCCTGCGGCAGTCCAG CTCCTACGAGGCGTGGTACTTCCTCCAGCCCCGGGACAATGGTAGCAAGAGCCTAAAGCCAGACGACCTG GGCTCCCTGCGGCTGAACGTGGTATACACGGAAGACCACGTGTTTTCTTCTGACTATTACAGCCCTCTGC GGGACCTGCTGTTGAAGTCTGCGGATGTGGAGCCCGTGTCAGCGTCTGCGGCCCACATCCTGGGCGAGGT TTGCCGGGAGAAGCAGGAGGCGGCCGTCCCGCTGGTGCGGCTCTTCCTACACTATGGCAGGGTGGTGCCA TTCATCAGTGCCATCGCCAGCGCGGAGGTGAAGCGGACCCAGGACCCCAACACCATCTTCCGAGGAAACT CACTGGCGTCCAAGTGCATCGACGAGACCATGAAGCTGGCGGGGATGCATTACCTGCATGTCACCCTGAA GCCCGCCATCGAGGAGATATGCCAGAGCCACAAACCCTGTGAAATCGACCCTGTGAAGTTGAAAGACGGA GAAAACCTTGAAAACAACATGGAGAACCTACGGCAGTATGTGGACCGCGTCTTCCACGCCATCACCGAGT CTGGGGTGAGCTGCCCGACCGTCATGTGTGACATCTTCTTCTCCCTCCGGGAGGCGGCGGCCAAGCGCTT CCAGGATGACCCGGACGTCAGGTACACTGCAGTGAGCAGCTTCATCTTCCTGAGGTTCTTTGCGCCCGCC ATTCTCTCCCCCAACCTCTTCCAGCTCACGCCGCACCACACGGACCCCCAGACGTCCAGGACGCTGACAT TGATCTCCAAGACCGTTCAGACCCTCGGCAGCCTGTCCAAGTCCAAATCTGCGAGTTTTAAGGAGTCCTA CATGGCTACATTTTATGAATTCTTCAATGAGCAGAAATATGCTGATGCGGTGAAGAACTTCTTGGATCTG ATTTCGTCCTCGGGGAGAAGAGACCCCAAGAGTGTTGAGCAGCCCATCGTGCTTAAAGAAGGGTTCATGA TCAAGAGGGCCCAAGGACGGAAGCGCTTTGGGATGAAGAATTTTAAGAAGAGATGGTTTCGCTTGACCAA CCATGAATTTACCTACCACAAAAGCAAAGGGGACCAGCCTCTCTACAGCATTCCCATCGAGAACATCCTG GCAGTGGAGAAGCTGGAGGAGGAGTCTTTCAAAATGAAAAACATGTTCCAGGTCATCCAGCCAGAGCGTG CGCTGTACATCCAGGCCAACAACTGCGTGGAGGCCAAGGACTGGATCGACATTCTCACCAAAGTGAGCCA GTGCAACCAGAAGCGCCTCACCGTCTACCACCCGTCCGCCTACCTGAGCGGCCACTGGCTGTGCTGTAGG GCGCCATCCGACTCGGCTCCGGGCTGCTCGCCCTGCACTGGCGGCCTCCCAGCCAACATCCAGCTGGACA TTGATGGGGACCGTGAGACGGAGCGTATCTACTCCCTCTTCAACTTGTACATGAGCAAGCTGGAGAAGAT GCAGGAGGCCTGTGGGAGCAAATCTGTGTATGACGGCCCGGAGCAGGAGGAGTATTCGACGTTCGTCATT GACGACCCCCAGGAGACCTACAAGACGCTAAAGCAAGTCATCGCTGGGGTTGGGGCTTTGGAGCAGGAGC ACGCCCAGTATAAGAGGGACAAGTTCAAGAAGACGAAATATGGAAGCCAGGAGCACCCCATCGGAGACAA GAGCTTCCAGAACTACATCCGGCAGCAGTCCGAGACCTCCACTCATTCCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207848 protein sequence
Red=Cloning site Green=Tags(s) MAVEDEGLRVFQSVKIKIGEAKNLPSYPGPSKMRDCYCTVNLDQEEVFRTKIVEKSLCPFYGEDFYCEIP RSFRHLSFYIFDRDVFRRDSIIGKVAIQKEDLQKYHNRDTWFQLQHVDADSEVQGKVHLELRLSEVITDT GVVCHKLATRIVECQGLPIVNGQCDPYATVTLAGPFRSEAKKTKVKRKTNNPQFDEVFYFEVTRPCSYSK KSHFDFEEEDVDKLEIRVDLWNASNLKFGDEFLGELRIPLKVLRQSSSYEAWYFLQPRDNGSKSLKPDDL GSLRLNVVYTEDHVFSSDYYSPLRDLLLKSADVEPVSASAAHILGEVCREKQEAAVPLVRLFLHYGRVVP FISAIASAEVKRTQDPNTIFRGNSLASKCIDETMKLAGMHYLHVTLKPAIEEICQSHKPCEIDPVKLKDG ENLENNMENLRQYVDRVFHAITESGVSCPTVMCDIFFSLREAAAKRFQDDPDVRYTAVSSFIFLRFFAPA ILSPNLFQLTPHHTDPQTSRTLTLISKTVQTLGSLSKSKSASFKESYMATFYEFFNEQKYADAVKNFLDL ISSSGRRDPKSVEQPIVLKEGFMIKRAQGRKRFGMKNFKKRWFRLTNHEFTYHKSKGDQPLYSIPIENIL AVEKLEEESFKMKNMFQVIQPERALYIQANNCVEAKDWIDILTKVSQCNQKRLTVYHPSAYLSGHWLCCR APSDSAPGCSPCTGGLPANIQLDIDGDRETERIYSLFNLYMSKLEKMQEACGSKSVYDGPEQEEYSTFVI DDPQETYKTLKQVIAGVGALEQEHAQYKRDKFKKTKYGSQEHPIGDKSFQNYIRQQSETSTHSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007368 |
| ORF Size | 2502 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_007368.4 |
| RefSeq Size | 4220 bp |
| RefSeq ORF | 2505 bp |
| Locus ID | 22821 |
| UniProt ID | Q14644 |
| Domains | C2, BTK, PH, RasGAP |
| MW | 95.7 kDa |
| Gene Summary | This gene encodes a protein that binds inositol 1,3,4,5-tetrakisphosphate and stimulates the GTPase activity of Ras p21. This protein functions as a negative regulator of the Ras signalling pathway. It is localized to the cell membrane via a pleckstrin homology (PH) domain in the C-terminal region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207848L1 | Lenti ORF clone of Human RAS p21 protein activator 3 (RASA3), Myc-DDK-tagged |
CNY 8,512.00 |
|
| RC207848L2 | Lenti ORF clone of Human RAS p21 protein activator 3 (RASA3), mGFP tagged |
CNY 5,890.00 |
|
| RC207848L3 | Lenti ORF clone of Human RAS p21 protein activator 3 (RASA3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207848L4 | Lenti ORF clone of Human RAS p21 protein activator 3 (RASA3), mGFP tagged |
CNY 5,890.00 |
|
| RG207848 | RASA3 (tGFP-tagged) - Human RAS p21 protein activator 3 (RASA3) |
CNY 4,370.00 |
|
| SC112652 | RASA3 (untagged)-Human RAS p21 protein activator 3 (RASA3) |
CNY 6,120.00 |
