RASA3 (NM_007368) Human Untagged Clone

CAT#: SC112652

RASA3 (untagged)-Human RAS p21 protein activator 3 (RASA3)



  "NM_007368" in other vectors (6)

CNY 6,120.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


RASA3 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms GAP1IP4BP; GAPIII
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC112652 sequence for NM_007368 edited (data generated by NextGen Sequencing)
ATGGCGGTGGAGGACGAGGGGCTCCGGGTCTTCCAGAGCGTGAAGATCAAGATCGGTGAA
GCCAAAAACCTTCCCTCTTACCCGGGGCCGAGCAAGATGAGGGATTGCTACTGCACGGTG
AACCTGGACCAGGAGGAGGTTTTCAGGACCAAAATTGTGGAAAAGTCACTCTGCCCGTTT
TACGGAGAAGACTTTTACTGTGAAATTCCTCGGAGCTTTCGTCACCTGTCCTTCTACATT
TTCGATAGAGACGTTTTCCGGAGGGATTCCATCATAGGGAAGGTGGCCATCCAGAAGGAG
GACTTGCAGAAGTACCACAACAGGGACACCTGGTTCCAGCTGCAGCACGTGGACGCTGAC
TCGGAAGTGCAGGGCAAAGTGCACCTGGAGCTGCGGCTGAGCGAGGTCATCACAGACACT
GGGGTCGTCTGCCACAAGCTCGCCACACGCATCGTCGAGTGCCAGGGCCTCCCCATCGTG
AATGGGCAATGTGACCCCTACGCCACCGTGACGCTGGCAGGACCCTTCAGATCAGAAGCA
AAGAAGACGAAAGTGAAGAGGAAGACCAACAATCCCCAGTTCGATGAAGTGTTTTATTTT
GAGGTGACCCGGCCCTGTAGCTACAGCAAGAAGTCCCACTTTGACTTTGAGGAGGAAGAC
GTGGACAAGCTCGAAATCAGAGTTGACCTCTGGAATGCCAGTAACCTGAAGTTTGGAGAT
GAATTCCTGGGAGAACTAAGGATCCCGTTGAAAGTCCTGCGGCAGTCCAGCTCCTACGAG
GCGTGGTACTTCCTCCAGCCCCGGGACAATGGTAGCAAGAGCCTAAAGCCAGACGACCTG
GGCTCCCTGCGGCTGAACGTGGTATACACGGAAGACCACGTGTTTTCTTCTGACTATTAC
AGCCCTCTGCGGGACCTGCTGTTGAAGTCTGCGGATGTGGAGCCCGTGTCAGCGTCTGCG
GCCCACATCCTGGGCGAGGTTTGCCGGGAGAAGCAGGAGGCGGCCGTCCCGCTGGTGCGG
CTCTTCCTACACTATGGCAGGGTGGTGCCATTCATCAGTGCCATCGCCAGCGCGGAGGTG
AAGCGGACCCAGGACCCCAACACCATCTTCCGAGGAAACTCACTGGCGTCCAAGTGCATC
GACGAGACCATGAAGCTGGCGGGGATGCATTACCTGCATGTCACCCTGAAGCCCGCCATC
GAGGAGATATGCCAGAGCCACAAACCCTGTGAAATCGACCCTGTGAAGTTGAAAGACGGA
GAAAACCTTGAAAACAACATGGAGAACCTACGGCAGTATGTGGACCGCGTCTTCCACGCC
ATCACCGAGTCTGGGGTGAGCTGCCCGACCGTCATGTGTGACATCTTCTTCTCCCTCCGG
GAGGCGGCGGCCAAGCGCTTCCAGGATGACCCGGACGTCAGGTACACTGCAGTGAGCAGC
TTCATCTTCCTGAGGTTCTTTGCGCCCGCCATTCTCTCCCCCAACCTCTTCCAGCTCACG
CCGCACCACACGGACCCCCAGACGTCCAGGACGCTGACATTGATCTCCAAGACCGTTCAG
ACCCTCGGCAGCCTGTCCAAGTCCAAATCTGCGAGTTTTAAGGAGTCCTACATGGCTACA
TTTTATGAATTCTTCAATGAGCAGAAATATGCTGATGCGGTGAAGAACTTCTTGGATCTG
ATTTCGTCCTCGGGGAGAAGAGACCCCAAGAGTGTTGAGCAGCCCATCGTGCTTAAAGAA
GGGTTCATGATCAAGAGGGCCCAAGGACGGAAGCGCTTTGGGATGAAGAATTTTAAGAAG
AGATGGTTTCGCTTGACCAACCATGAATTTACCTACCACAAAAGCAAAGGGGACCAGCCT
CTCTACAGCATTCCCATCGAGAACATCCTGGCAGTGGAGAAGCTGGAGGAGGAGTCTTTC
AAAATGAAAAACATGTTCCAGGTCATCCAGCCAGAGCGTGCGCTGTACATCCAGGCCAAC
AACTGCGTGGAGGCCAAGGACTGGATCGACATTCTCACCAAAGTGAGCCAGTGCAACCAG
AAGCGCCTCACCGTCTACCACCCGTCCGCCTACCTGAGCGGCCACTGGCTGTGCTGTAGG
GCGCCATCCGACTCGGCTCCGGGCTGCTCGCCCTGCACTGGCGGCCTCCCAGCCAACATC
CAGCTGGACATTGATGGGGACCGTGAGACGGAGCGTATCTACTCCCTCTTCAACTTGTAC
ATGAGCAAGCTGGAGAAGATGCAGGAGGCCTGTGGGAGCAAATCTGTGTATGACGGCCCG
GAGCAGGAGGAGTATTCGACGTTCGTCATTGACGACCCCCAGGAGACCTACAAGACGCTA
AAGCAAGTCATCGCTGGGGTTGGGGCTTTGGAGCAGGAGCACGCCCAGTATAAGAGGGAC
AAGTTCAAGAAGACGAAATATGGAAGCCAGGAGCACCCCATCGGAGACAAGAGCTTCCAG
AACTACATCCGGCAGCAGTCCGAGACCTCCACTCATTCCATTTAA

Clone variation with respect to NM_007368.2
1326 t=>c
>OriGene 5' read for NM_007368 unedited
ATAGGGCGGCCGCGAATTCGGCACGAGGCTGAGCTGCCCGGGCTCGGCGCGCGCTTGGGG
CGAGGCTCGGCGGGCGCGGACGCGCAGCATGGCGGTGGAGGACGAGGGGCTCCGGGTCTT
CCAGAGCGTGAAGATCAAGATCGGTGAAGCCAAAAACCTTCCCTCTTACCCGGGGCCGAG
CAAGATGAGGGATTGCTACTGCACGGTGAACCTGGACCAGGAGGAGGTTTTCAGGACCAA
AATTGTGGAAAAGTCACTCTGCCCGTTTTACGGAGAAGACTTTTACTGTGAAATTCCTCG
GAGCTTTCGTCACCTGTCCTTCTACATTTTCGATAGAGACGTTTTCCGGAGGGATTCCAT
CATAGGGAAGGTGGCCATCCAGAAGGAGGACTTGCAGAAGTACCACAACAGGGACACCTG
GTTCCAGCTGCAGCACGTGGACGCTGACTCGGAAGTGCAGGGCAAAGTGCACCTGGAGCT
GCGGCTGAGCGAGGTCATCACAGNACACTGGGGTCGTCTGCCACAAGCTCGCCACACGCA
TCGTCGAGTGCCAGGGCCTCCCCATCGTGAATGGGCAATGTGACCCCTACGCCACCGTGA
CGCTGGCAGGACCCTTCAGATCAGAAGCANAGAAGACGAAAGTGAAGAGGAAGAACCAAC
ATCCCCAGTTCGATGAAGTGTTTTATTTTGAGGTGACCCCGGCCCTGTAGCTACAGCAAG
AAGTCCCACTTTGACTTTGAGGAGGAAGACGTGGNACAGCTCGAAATCAGAGTTGACCTC
TGGAATGCCAGTACCCTGAAGTTTGGAGATGAATTCTGGGAGAACTAAGATNCCGTTGAA
GTCCTGCGGCATCCAGTTCTACCAGGCGTGTACTTTCTCCAGCCCGGGACATGGTACAGA
GCCTAACCAGACACTGNGCTCCTGGG
>OriGene 3' read for NM_007368 unedited
TAAACATCTGTTTTAATTGGGGCCAGTAAAATACATTGGCAAATCATTAGTATTTTATAA
ACAGAAACATTAAGTTATAAATCCATGTTAAAAATTGCAGCTCAGTGCAGCCCCAACAGC
TGTGTGTCCAAAAGTCTATTTTTACAACATTTTCCTCCAGTATTTTTGTGACAAGAAAGT
TCCTATCTGCCATCTAGATCAAGAGCCTGAACTACAGTATTTTCAAACAAGAACCGGGAG
AAGTCGGTACAAGGATACGTCGTGCTGTGTATTTAAACGGCGCTTCCCACACACCTGCCG
TCTGCATCCCGGGAGGACGCCCTTTGGGAAAGGCCTCCTCGGCAGTGTGAGGTCTGGAGG
GGTCTGCCTGGACCCCATTTTTAGGATGCTCCCCTCCCCAATAAAACAGCAGCCCCCGCC
CCTGCCCTGCACAGCATCTGGGGGTTCATAACACAGGTGCAATACTGACCATGGGCCCTG
GAGGGACGTCTGCACCCCGAAGCCCCTGTACCTCAAACTCAGAGTTTCTTCCCTTCTTTG
ATTTTCTGGAGGACCTGCAGCTGGCCTTCCTGAGACAGGCTCCATTCCTGTTCCATTTGC
CTTCCCGGCAGCCTTCCCTCTAGTGGGTATAGGTTCTGACGTTCTGAGTTACTTTGTATC
AAAGAGCTAATTAAAAATGGTTCTTCACAAACATAAAGAAAAACAGCTTGAAACGGTACA
TCTTCCTATTTTTAAATCTTCAACCTTCCACGGGGGGGTGGCCAATGTTAATTCTGATTT
TAAACCCCTTTAACAGCAAACGATGTGGGCTTCAACTGTGGGCTTCCGAACACCCTGGAG
ACCTGGAGCCACCCTTTTCCTCCAGGGACTCTGGGCAAACCTGTTTCCCCAGCCCAAGCA
CTCTCAACTGGACTTTGGGTATCTGCCTCCAGCTTAACGGCCCTATCGGAATAACAACCC
GAGGGGACGGGGCCGCTTCTGTTG
Restriction Sites NotI-NotI     
ACCN NM_007368
Insert Size 4110 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_007368.2, NP_031394.2
RefSeq Size 4220 bp
RefSeq ORF 2505 bp
Locus ID 22821
UniProt ID Q14644
Domains C2, BTK, PH, RasGAP
Gene Summary This gene encodes a protein that binds inositol 1,3,4,5-tetrakisphosphate and stimulates the GTPase activity of Ras p21. This protein functions as a negative regulator of the Ras signalling pathway. It is localized to the cell membrane via a pleckstrin homology (PH) domain in the C-terminal region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Transcript Variant: This variant (1) encodes the longest isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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