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CD56 (NCAM1) (NM_181351) Human Tagged ORF Clone
CAT＃: RC207890
- TrueORF®
NCAM1 (Myc-DDK-tagged)-Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2
ORF Plasmid: tGFP
"NM_181351" in other vectors (6)
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CNY 9,424.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD56; MSK39; NCAM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207890 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAAACTAAGGATCTCATCTGGACTTTGTTTTTCCTGGGAACTGCAGTTTCTCTGCAGGTGGATA TTGTTCCCAGCCAGGGGGAGATCAGCGTTGGAGAGTCCAAATTCTTCTTATGCCAAGTGGCAGGAGATGC CAAAGATAAAGACATCTCCTGGTTCTCCCCCAATGGAGAAAAGCTCACCCCAAACCAGCAGCGGATCTCA GTGGTGTGGAATGATGATTCCTCCTCCACCCTCACCATCTATAACGCCAACATCGACGACGCCGGCATTT ACAAGTGTGTGGTTACAGGCGAGGATGGCAGTGAGTCAGAGGCCACCGTCAACGTGAAGATCTTTCAGAA GCTCATGTTCAAGAATGCGCCAACCCCACAGGAGTTCCGGGAGGGGGAAGATGCCGTGATTGTGTGTGAT GTGGTCAGCTCCCTCCCACCAACCATCATCTGGAAACACAAAGGCCGAGATGTCATCCTGAAAAAAGATG TCCGATTCATAGTCCTGTCCAACAACTACCTGCAGATCCGGGGCATCAAGAAAACAGATGAGGGCACTTA TCGCTGTGAGGGCAGAATCCTGGCACGGGGGGAGATCAACTTCAAGGACATTCAGGTCATTGTGAATGTG CCACCTACCATCCAGGCCAGGCAGAATATTGTGAATGCCACCGCCAACCTCGGCCAGTCCGTCACCCTGG TGTGCGATGCCGAAGGCTTCCCAGAGCCCACCATGAGCTGGACAAAGGATGGGGAACAGATAGAGCAAGA GGAAGACGATGAGAAGTACATCTTCAGCGACGATAGTTCCCAGCTGACCATCAAAAAGGTGGATAAGAAC GACGAGGCTGAGTACATCTGCATTGCTGAGAACAAGGCTGGCGAGCAGGATGCGACCATCCACCTCAAAG TCTTTGCAAAACCCAAAATCACATATGTAGAGAACCAGACTGCCATGGAATTAGAGGAGCAGGTCACTCT TACCTGTGAAGCCTCCGGAGACCCCATTCCCTCCATCACCTGGAGGACTTCTACCCGGAACATCAGCAGC GAAGAAAAGGCTTCGTGGACTCGACCAGAGAAGCAAGAGACTCTGGATGGGCACATGGTGGTGCGTAGCC ATGCCCGTGTGTCGTCGCTGACCCTGAAGAGCATCCAGTACACTGATGCCGGAGAGTACATCTGCACCGC CAGCAACACCATCGGCCAGGACTCCCAGTCCATGTACCTTGAAGTGCAATATGCCCCAAAGCTACAGGGC CCTGTGGCTGTGTACACTTGGGAGGGGAACCAGGTGAACATCACCTGCGAGGTATTTGCCTATCCCAGTG CCACGATCTCATGGTTTCGGGATGGCCAGCTGCTGCCAAGCTCCAATTACAGCAATATCAAGATCTACAA CACCCCCTCTGCCAGCTATCTGGAGGTGACCCCAGACTCTGAGAATGATTTTGGGAACTACAACTGTACT GCAGTGAACCGCATTGGGCAGGAGTCCTTGGAATTCATCCTTGTTCAAGCAGACACCCCCTCTTCACCAT CCATCGACCAGGTGGAGCCATACTCCAGCACAGCCCAGGTGCAGTTTGATGAACCAGAGGCCACAGGTGG GGTGCCCATCCTCAAATACAAAGCTGAGTGGAGAGCAGTGGGTGAAGAAGTATGGCATTCCAAGTGGTAT GATGCCAAGGAAGCCAGCATGGAGGGCATCGTCACCATCGTGGGCCTGAAGCCCGAAACAACGTACGCCG TAAGGCTGGCGGCGCTCAATGGCAAAGGGCTGGGTGAGATCAGCGCGGCCTCCGAGTTCAAGACGCAGCC AGTCCAAGGGGAACCCAGTGCACCTAAGCTCGAAGGGCAGATGGGAGAGGATGGAAACTCTATTAAAGTG AACCTGATCAAGCAGGATGACGGCGGCTCCCCCATCAGACACTATCTGGTCAGGTACCGAGCGCTCTCCT CCGAGTGGAAACCAGAGATCAGGCTCCCGTCTGGCAGTGACCACGTCATGCTGAAGTCCCTGGACTGGAA TGCTGAGTATGAGGTCTACGTGGTGGCTGAGAACCAGCAAGGAAAATCCAAGGCGGCTCATTTTGTGTTC AGGACCTCGGCCCAGCCCACAGCCATCCCAGCCAACGGCAGCCCCACCTCAGGCCTGAGCACCGGGGCCA TCGTGGGCATCCTCATCGTCATCTTCGTCCTGCTCCTGGTGGTTGTGGACATCACCTGCTACTTCCTGAA CAAGTGTGGCCTGTTCATGTGCATTGCGGTCAACCTGTGTGGAAAAGCCGGGCCCGGGGCCAAGGGCAAG GACATGGAGGAGGGCAAGGCCGCCTTCTCGAAAGATGAGTCCAAGGAGCCCATCGTGGAGGTTCGAACGG AGGAGGAGAGGACCCCAAACCATGATGGAGGGAAACACACAGAGCCCAACGAGACCACGCCACTGACGGA GCCCGAGAAGGGCCCCGTAGAAGCAAAGCCAGAGTGCCAGGAGACAGAAACGAAGCCAGCGCCAGCCGAA GTCAAGACGGTCCCCAATGACGCCACACAGACAAAGGAGAACGAGAACAAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207890 protein sequence
Red=Cloning site Green=Tags(s) MLQTKDLIWTLFFLGTAVSLQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLTPNQQRIS VVWNDDSSSTLTIYNANIDDAGIYKCVVTGEDGSESEATVNVKIFQKLMFKNAPTPQEFREGEDAVIVCD VVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNV PPTIQARQNIVNATANLGQSVTLVCDAEGFPEPTMSWTKDGEQIEQEEDDEKYIFSDDSSQLTIKKVDKN DEAEYICIAENKAGEQDATIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISS EEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQG PVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCT AVNRIGQESLEFILVQADTPSSPSIDQVEPYSSTAQVQFDEPEATGGVPILKYKAEWRAVGEEVWHSKWY DAKEASMEGIVTIVGLKPETTYAVRLAALNGKGLGEISAASEFKTQPVQGEPSAPKLEGQMGEDGNSIKV NLIKQDDGGSPIRHYLVRYRALSSEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVF RTSAQPTAIPANGSPTSGLSTGAIVGILIVIFVLLLVVVDITCYFLNKCGLFMCIAVNLCGKAGPGAKGK DMEEGKAAFSKDESKEPIVEVRTEEERTPNHDGGKHTEPNETTPLTEPEKGPVEAKPECQETETKPAPAE VKTVPNDATQTKENENKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181351 |
| ORF Size | 2574 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_181351.1, NP_851996.1 |
| RefSeq Size | 6007 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 4684 |
| UniProt ID | P13591 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Prion diseases |
| MW | 94.6 kDa |
| Gene Summary | This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein plays a role in the development of the nervous system by regulating neurogenesis, neurite outgrowth, and cell migration. This protein is also involved in the expansion of T lymphocytes, B lymphocytes and natural killer (NK) cells which play an important role in immune surveillance. This protein plays a role in signal transduction by interacting with fibroblast growth factor receptors, N-cadherin and other components of the extracellular matrix and by triggering signalling cascades involving FYN-focal adhesion kinase (FAK), mitogen-activated protein kinase (MAPK), and phosphatidylinositol 3-kinase (PI3K). One prominent isoform of this gene, cell surface molecule CD56, plays a role in several myeloproliferative disorders such as acute myeloid leukemia and differential expression of this gene is associated with differential disease progression. For example, increased expression of CD56 is correlated with lower survival in acute myeloid leukemia patients whereas increased severity of COVID-19 is correlated with decreased abundance of CD56-expressing NK cells in peripheral blood. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Aug 2020] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Neural cell adhesion molecule 1 is a novel autoantigen in membranous lupus nephritis
,Caza, T;Hassen, S;Kuperman, M;Sharma, S;Dvanajscak, Z;Arthur, J;Edmondson, R;Storey, A;Herzog, C;Kenan, D;Larsen, C;,
Kidney Int
,PubMed ID 33045259
[CD56]
|
|
Differential killing of CD56-expressing cells by drug-conjugated human antibodies targeting membrane-distal and membrane-proximal non-overlapping epitopes
,Feng, Y;Wang, Y;Zhu, Z;Li, W;Sussman, RT;Randall, M;Bosse, KR;Maris, JM;Dimitrov, DS;,
MAbs
,PubMed ID 26910291
[CD56]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207890L1 | Lenti ORF clone of Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2, Myc-DDK-tagged |
CNY 11,824.00 |
|
| RC207890L2 | Lenti ORF clone of Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC207890L3 | Lenti ORF clone of Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2, Myc-DDK-tagged |
CNY 11,824.00 |
|
| RC207890L4 | Lenti ORF clone of Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2, mGFP tagged |
CNY 11,824.00 |
|
| RG207890 | NCAM1 (tGFP-tagged) - Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2 |
CNY 11,024.00 |
|
| SC121351 | NCAM1 (untagged)-Human neural cell adhesion molecule 1 (NCAM1), transcript variant 2 |
CNY 9,432.00 |
