NALP4 (NLRP4) (NM_134444) Human Tagged ORF Clone
CAT#: RC207991
NLRP4 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 4 (NLRP4)
ORF Plasmid: tGFP
"NM_134444" in other vectors (6)
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CNY 7,280.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | CLR19.5; CT58; NALP4; PAN2; PYPAF4; RNH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC207991 representing NM_134444
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCTCTTTCTTCTCTGATTTTGGTCTTATGTGGTATCTGGAGGAGCTCAAAAAGGAGGAGTTCA GGAAATTTAAAGAACATCTCAAGCAAATGACTTTGCAGCTTGAACTCAAGCAGATTCCCTGGACTGAGGT CAAAAAAGCATCCCGGGAAGAACTTGCAAACCTCTTGATCAAGCACTATGAAGAACAACAAGCTTGGAAC ATAACCTTAAGAATCTTTCAAAAGATGGATAGAAAGGATCTCTGCATGAAGGTCATGAGGGAGAGAACAG GATACACAAAGACCTATCAAGCTCACGCAAAGCAGAAATTCAGCCGCTTATGGTCCAGCAAGTCTGTCAC TGAGATTCACCTATACTTTGAGGAGGAAGTCAAGCAAGAAGAATGTGACCATTTGGACCGCCTTTTTGCT CCCAAGGAAGCTGGGAAACAGCCACGTACAGTGATCATTCAAGGACCACAAGGAATTGGAAAAACGACAC TCCTGATGAAGCTGATGATGGCCTGGTCGGACAACAAGATCTTTCGGGATAGGTTCCTGTACACATTCTA TTTCTGCTGCAGAGAACTGAGGGAGTTGCCGCCAACGAGTTTGGCTGACTTGATTTCCAGAGAGTGGCCT GACCCCGCTGCTCCTATAACAGAGATCGTGTCTCAACCGGAGAGACTCTTGTTCGTCATCGACAGCTTCG AAGAGCTGCAGGGCGGCTTGAACGAACCCGATTCGGATCTGTGTGGTGACTTGATGGAGAAACGGCCGGT GCAGGTGCTTCTGAGCAGTTTGCTGAGGAAGAAGATGCTCCCGGAGGCCTCCCTGCTCATCGCTATCAAA CCCGTGTGCCCGAAGGAGCTCCGGGATCAGGTGACGATCTCAGAAATCTACCAGCCCCGGGGATTCAACG AGAGTGATAGGTTAGTGTATTTCTGCTGTTTCTTCAAAGACCCGAAAAGAGCCATGGAAGCCTTCAATCT TGTAAGAGAAAGTGAACAGCTGTTTTCCATATGCCAAATCCCGCTCCTCTGCTGGATCCTGTGTACCAGT CTGAAGCAAGAGATGCAGAAAGGAAAAGACCTGGCCCTGACCTGCCAGAGCACTACCTCTGTGTACTCCT CTTTCGTCTTTAACCTGTTCACACCTGACGGTGCCGAGGGCCCGACTCAGCAAACCCAGCACCAGCTGAA GGCCCTGTGCTCCCTGGCTGCAGAGGGTATGTGGACAGACACATTTGAGTTTTGTGAAGACGACCTCCGG AGAAATGGGGTTGTTGACGCTGACATCCCTGCGCTGCTGGGCACCAAGATACTTCTGAAGTACGGGGAGC GTGAGAGCTCCTACGTGTTCCTCCACGTGTGTATCCAGGAGTTCTGTGCCGCCTTGTTCTATTTGCTCAA GAGCCACCTTGATCATCCTCACCCAGCTGTGAGATGTGTACAGGAATTGCTAGTTGCCAATTTTGAAAAA GCAAGGAGAGCACATTGGATTTTTTTGGGGTGTTTTCTAACTGGCCTTTTAAATAAAAAGGAACAAGAAA AACTGGATGCGTTTTTTGGCTTCCAACTGTCCCAAGAGATAAAGCAGCAAATTCACCAGTGCCTGAAGAG CTTAGGGGAGCGTGGCAATCCTCAGGGACAGGTGGATTCCTTGGCGATATTTTACTGTCTCTTTGAAATG CAGGATCCTGCCTTTGTGAAGCAGGCAGTGAACCTCCTCCAAGAAGCTAACTTTCATATTATTGACAACG TGGACTTGGTGGTTTCTGCCTACTGCTTAAAATACTGCTCCAGCTTGAGGAAACTCTGTTTTTCCGTTCA AAATGTCTTTAAGAAAGAGGATGAACACAGCTCTACGTCGGATTACAGCCTCATCTGTTGGCATCACATC TGCTCTGTGCTCACCACCAGCGGGCACCTCAGAGAGCTCCAGGTGCAGGACAGCACCCTCAGCGAGTCGA CCTTTGTGACCTGGTGTAACCAGCTGAGGCATCCCAGCTGTCGCCTTCAGAAGCTTGGAATAAATAACGT TTCCTTTTCTGGCCAGAGTGTTCTGCTCTTTGAGGTGCTCTTTTATCAGCCAGACTTGAAATACCTGAGC TTCACCCTCACGAAACTCTCTCGTGATGACATCAGGTCCCTCTGTGATGCCTTGAACTACCCAGCAGGCA ACGTCAAAGAGCTAGCGCTGGTAAATTGTCACCTCTCACCCATTGATTGTGAAGTCCTTGCTGGCCTTCT AACCAACAACAAGAAGCTGACGTATCTGAATGTATCCTGCAACCAGTTAGACACAGGCGTGCCCCTTTTG TGTGAAGCCATGTGCAGCCCAGACACGGTCCTGGTATACCTGATGTTGGCTTTCTGCCACCTCAGCGAGC AGTGCTGCGAATACATCTCTGAAATGCTTCTGCGTAACAAGAGCGTGCGCTATCTAGACCTCAGTGCCAA TGTCCTGAAGGACGAAGGACTGAAAACTCTCTGCGAGGCCTTGAAACATCCGGACTGCTGCCTGGATTCA CTGTGTTTGGTAAAATGTTTTATCACTGCTGCTGGCTGTGAAGACCTCGCCTCTGCTCTCATCAGCAATC AAAACCTGAAGATTCTGCAAATTGGGTGCAATGAAATCGGAGATGTGGGTGTGCAGCTGTTGTGTCGGGC TCTGACGCATACGGATTGCCGCTTAGAGATTCTTGGGTTGGAAGAATGTGGGTTAACGAGCACCTGCTGT AAGGATCTCGCGTCTGTTCTCACCTGCAGTAAGACCCTGCAGCTGCTCAACCTGACCTTGAACACCTTGG ACCACACAGGGGTGGTTGTACTCTGTGAGGCCCTGAGACACCCAGAGTGTGCCCTGCAGGTGCTCGGGCT GAGAAAAACTGATTTTGATGAGGAAACCCAGGCACTGCTGACGGCTGAGGAAGAGAGAAATCCTAACCTG ACCATCACAGACGACTGTGACACAATCACAAGGGTAGAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207991 representing NM_134444
Red=Cloning site Green=Tags(s) MAASFFSDFGLMWYLEELKKEEFRKFKEHLKQMTLQLELKQIPWTEVKKASREELANLLIKHYEEQQAWN ITLRIFQKMDRKDLCMKVMRERTGYTKTYQAHAKQKFSRLWSSKSVTEIHLYFEEEVKQEECDHLDRLFA PKEAGKQPRTVIIQGPQGIGKTTLLMKLMMAWSDNKIFRDRFLYTFYFCCRELRELPPTSLADLISREWP DPAAPITEIVSQPERLLFVIDSFEELQGGLNEPDSDLCGDLMEKRPVQVLLSSLLRKKMLPEASLLIAIK PVCPKELRDQVTISEIYQPRGFNESDRLVYFCCFFKDPKRAMEAFNLVRESEQLFSICQIPLLCWILCTS LKQEMQKGKDLALTCQSTTSVYSSFVFNLFTPDGAEGPTQQTQHQLKALCSLAAEGMWTDTFEFCEDDLR RNGVVDADIPALLGTKILLKYGERESSYVFLHVCIQEFCAALFYLLKSHLDHPHPAVRCVQELLVANFEK ARRAHWIFLGCFLTGLLNKKEQEKLDAFFGFQLSQEIKQQIHQCLKSLGERGNPQGQVDSLAIFYCLFEM QDPAFVKQAVNLLQEANFHIIDNVDLVVSAYCLKYCSSLRKLCFSVQNVFKKEDEHSSTSDYSLICWHHI CSVLTTSGHLRELQVQDSTLSESTFVTWCNQLRHPSCRLQKLGINNVSFSGQSVLLFEVLFYQPDLKYLS FTLTKLSRDDIRSLCDALNYPAGNVKELALVNCHLSPIDCEVLAGLLTNNKKLTYLNVSCNQLDTGVPLL CEAMCSPDTVLVYLMLAFCHLSEQCCEYISEMLLRNKSVRYLDLSANVLKDEGLKTLCEALKHPDCCLDS LCLVKCFITAAGCEDLASALISNQNLKILQIGCNEIGDVGVQLLCRALTHTDCRLEILGLEECGLTSTCC KDLASVLTCSKTLQLLNLTLNTLDHTGVVVLCEALRHPECALQVLGLRKTDFDEETQALLTAEEERNPNL TITDDCDTITRVEI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_134444 |
ORF Size | 2982 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_134444.5 |
RefSeq Size | 3339 bp |
RefSeq ORF | 2985 bp |
Locus ID | 147945 |
UniProt ID | Q96MN2 |
MW | 113.2 kDa |
Gene Summary | The protein encoded by this gene is a member of the nucleotide-binding and leucine-rich repeat receptor (NLR) family, and is predicted to contain an N-terminal pyrin effector domain (PYD), a centrally-located nucleotide-binding and oligomerization domain (NACHT) and C-terminal leucine-rich repeats (LRR). This gene product has a demonstrated role as a negative regulator of autophagy and type I interferon signaling pathways as a result of protein interactions with its NACHT domain. The PYD domain has also been shown to be important in the inhibition of NF-kB (nuclear factor kappa-light-chain-enhancer of activated B cells). [provided by RefSeq, Dec 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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DYRK2 Negatively Regulates Type I Interferon Induction by Promoting TBK1 Degradation via Ser527 Phosphorylation
,An, T;Li, S;Pan, W;Tien, P;Zhong, B;Shu, HB;Wu, S;,
PLoS Pathog.
,PubMed ID 26407194
[NALP4]
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Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC207991L1 | Lenti ORF clone of Human NLR family, pyrin domain containing 4 (NLRP4), Myc-DDK-tagged |
CNY 9,680.00 |
|
RC207991L2 | Lenti ORF clone of Human NLR family, pyrin domain containing 4 (NLRP4), mGFP tagged |
CNY 5,890.00 |
|
RC207991L3 | Lenti ORF clone of Human NLR family, pyrin domain containing 4 (NLRP4), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC207991L4 | Lenti ORF clone of Human NLR family, pyrin domain containing 4 (NLRP4), mGFP tagged |
CNY 5,890.00 |
|
RG207991 | NLRP4 (tGFP-tagged) - Human NLR family, pyrin domain containing 4 (NLRP4) |
CNY 8,880.00 |
|
SC305988 | NLRP4 (untagged)-Human NLR family, pyrin domain containing 4 (NLRP4) |
CNY 7,288.00 |