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NUP153 (NM_005124) Human Tagged ORF Clone
CAT＃: RC208264
- TrueORF®
NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153)
ORF Plasmid: tGFP
"NM_005124" in other vectors (6)
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CNY 15,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HNUP153; N153 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208264 representing NM_005124
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCGGGAGCCGGAGGAGTCGGAGGGGGCGGTGGCGGCAAGATCCGGACGCGGCGTTGCCACCAGG GGCCAATTAAGCCTTACCAGCAGGGGCGACAACAGCATCAGGGCATTCTTAGCAGGGTTACAGAATCTGT TAAGAATATTGTGCCAGGGTGGCTACAAAGATACTTCAACAAGAATGAAGATGTATGCAGCTGTTCAACA GACACAAGCGAGGTTCCACGCTGGCCAGAAAATAAAGAGGACCATCTGGTATATGCCGATGAGGAGAGCT CTAATATTACTGATGGGAGAATCACACCTGAGCCAGCAGTCAGTAATACAGAAGAACCTTCAACAACTAG TACTGCTTCAAATTATCCAGATGTGTTAACAAGGCCTTCTCTTCATCGGAGCCATCTGAATTTTTCCATG TTGGAATCCCCTGCATTACACTGTCAGCCATCTACATCCTCGGCATTCCCAATTGGCAGTTCGGGATTTT CCCTTGTAAAGGAAATTAAAGATTCTACCTCTCAGCATGATGATGATAACATCTCAACTACCAGTGGTTT TTCTTCAAGAGCTTCTGATAAAGATATAACTGTTTCAAAGAACACTTCATTGCCACCTCTGTGGTCCCCA GAAGCTGAACGTTCTCACTCACTCTCACAGCACACTGCCACCAGCTCAAAAAAACCAGCATTCAACTTGT CTGCCTTTGGAACACTTTCCCCTTCACTTGGGAATTCTTCAATCCTTAAAACCAGTCAGCTTGGAGATTC TCCTTTTTATCCTGGAAAAACAACATACGGTGGGGCAGCAGCTGCTGTAAGACAGTCTAAACTACGAAAT ACACCTTATCAGGCACCAGTTAGAAGACAAATGAAAGCTAAGCAACTCAGTGCACAATCTTACGGTGTGA CCAGTTCAACAGCTCGGCGAATATTGCAGTCTTTAGAGAAGATGTCAAGCCCTTTAGCGGATGCAAAAAG AATTCCATCCATTGTTTCTTCTCCTCTGAATTCTCCTCTTGATAGGAGTGGGATAGATATCACAGATTTT CAGGCCAAAAGAGAAAAGGTGGATTCTCAATATCCTCCTGTTCAGAGACTTATGACCCCAAAGCCAGTTT CCATAGCAACAAATCGAAGTGTTTATTTTAAACCATCTCTGACTCCTTCTGGTGAATTCAGGAAGACTAA TCAAAGAATAGATAACAAGTGCAGTACTGGATATGAAAAAAATATGACACCCGGACAAAATAGAGAACAA CGAGAAAGTGGCTTTTCATATCCAAATTTCAGTTTGCCTGCAGCCAATGGTTTATCTTCTGGAGTAGGTG GTGGAGGTGGCAAGATGAGACGAGAAAGAACACGCTTTGTTGCTTCTAAACCTCTGGAGGAGGAGGAAAT GGAAGTTCCAGTATTACCGAAAATCTCTCTACCGATCACCAGTTCTTCACTGCCTACCTTTAATTTTAGT TCCCCTGAGATCACAACTTCCTCTCCATCACCCATCAATTCGTCTCAAGCATTAACAAACAAGGTACAAA TGACCTCTCCGAGCAGCACTGGCAGTCCCATGTTTAAATTTTCATCTCCAATCGTAAAATCTACTGAGGC AAATGTACTACCTCCATCATCTATTGGATTTACATTTAGTGTGCCTGTTGCAAAAACAGCAGAACTTTCT GGTTCTAGTAGTACTTTAGAACCAATTATAAGTAGTTCAGCTCATCATGTCACTACAGTGAACAGTACAA ATTGTAAGAAGACACCACCTGAAGATTGTGAGGGTCCTTTTAGACCTGCAGAAATCCTGAAAGAAGGAAG TGTTCTAGATATTCTGAAAAGCCCTGGTTTCGCATCGCCGAAGATAGATTCTGTTGCTGCTCAGCCCACC GCAACAAGCCCAGTAGTTTATACAAGACCAGCAATAAGTAGCTTTTCTTCTAGTGGAATTGGGTTTGGGG AGAGTTTAAAAGCTGGGTCATCATGGCAGTGTGATACATGTCTACTCCAGAACAAAGTTACAGACAACAA ATGCATAGCCTGTCAAGCAGCAAAATTGTCACCCAGAGATACTGCTAAACAGACTGGAATTGAAACACCA AATAAAAGTGGCAAAACAACTCTTTCTGCATCAGGGACAGGCTTTGGAGACAAATTTAAACCAGTGATAG GCACTTGGGATTGTGATACCTGTTTAGTGCAAAATAAACCTGAAGCAATAAAATGTGTAGCCTGTGAAAC ACCGAAACCTGGAACTTGTGTGAAGCGAGCCCTTACATTGACAGTGGTTTCGGAAAGTGCTGAGACTATG ACTGCTTCATCTTCCAGCTGCACTGTAACCACTGGTACCTTAGGATTTGGAGATAAATTCAAAAGGCCCA TTGGATCTTGGGAGTGTTCAGTATGCTGTGTTTCTAATAATGCAGAAGACAATAAGTGTGTGTCCTGTAT GTCTGAGAAACCAGGAAGTTCAGTACCTGCTTCAAGTAGCAGCACTGTACCTGTCTCTCTGCCTTCTGGA GGCTCTCTAGGATTGGAAAAGTTCAAGAAACCCGAGGGAAGCTGGGACTGTGAATTGTGCCTAGTGCAGA ATAAGGCAGACTCTACCAAATGTTTGGCATGTGAAAGTGCAAAGCCAGGCACAAAATCTGGGTTTAAAGG CTTTGACACATCTTCCTCATCTTCGAACTCAGCAGCCTCCTCATCCTTCAAATTTGGTGTCTCATCATCC TCTTCTGGGCCTTCTCAGACTTTAACAAGCACTGGAAATTTTAAATTTGGAGATCAGGGAGGATTCAAAA TAGGTGTGTCATCTGATTCTGGGTCTATAAACCCCATGAGTGAAGGCTTTAAATTTTCTAAACCAATAGG AGATTTTAAATTTGGAGTTTCATCTGAATCTAAGCCCGAAGAAGTTAAAAAAGATAGTAAGAATGATAAT TTTAAGTTTGGACTTTCTTCTGGTTTAAGCAACCCAGTTTCTTTAACTCCATTTCAATTTGGGGTATCTA ATCTTGGACAGGAAGAAAAGAAAGAGGAACTGCCCAAATCTTCCTCTGCAGGTTTTAGCTTTGGTACAGG TGTTATTAACTCCACCCCTGCTCCTGCTAACACCATAGTGACCTCTGAGAACAAGAGCAGCTTCAACCTT GGAACCATAGAAACCAAGAGTGCTTCAGTGGCTCCTTTCACATGTAAGACATCAGAAGCTAAAAAAGAAG AAATGCCTGCCACCAAAGGAGGATTCTCTTTTGGCAACGTGGAGCCTGCCTCTCTGCCATCTGCCTCAGT GTTTGTTTTGGGAAGGACAGAAGAGAAACAGCAAGAGCCTGTCACTTCTACTTCCCTAGTTTTTGGGAAG AAAGCTGACAATGAAGAGCCAAAGTGTCAACCAGTGTTTTCCTTTGGGAATTCAGAGCAAACCAAAGATG AGAATTCTTCAAAGTCCACATTTAGTTTTAGTATGACAAAACCATCTGAGAAGGAATCTGAACAGCCAGC AAAAGCCACTTTTGCCTTTGGAGCTCAAACTAGTACTACAGCTGATCAAGGTGCAGCAAAGCCAGTTTTT AGTTTCTTGAACAACAGTTCCTCTAGTTCAAGTACACCAGCCACTTCTGCTGGTGGTGGCATATTTGGTA GTTCCACCTCTTCCTCCAATCCACCTGTGGCTACCTTTGTGTTTGGACAGTCCAGCAATCCTGTGAGCAG CTCTGCCTTTGGTAACACTGCTGAATCCAGCACCTCTCAGTCTTTGCTATTTTCTCAAGATAGCAAACTA GCAACCACATCCAGCACAGGTACAGCTGTCACCCCATTTGTCTTTGGTCCAGGAGCCAGCAGTAATAATA CTACCACCTCTGGTTTCGGCTTTGGAGCCACAACCACATCTAGCTCTGCAGGATCCTCCTTTGTATTTGG AACTGGACCCTCAGCACCATCTGCCAGTCCAGCATTTGGTGCTAACCAGACCCCAACATTTGGACAAAGT CAAGGTGCCAGCCAGCCCAATCCCCCAGGCTTTGGATCTATATCATCTTCCACAGCATTATTTCCCACTG GTTCTCAGCCTGCACCACCTACTTTTGGGACAGTGTCAAGCAGTAGCCAGCCCCCTGTGTTTGGACAGCA ACCTAGTCAGTCTGCATTTGGCTCTGGAACAACTCCTAATTCTAGTTCGGCTTTCCAGTTTGGCAGCAGC ACTACAAATTTCAACTTCACAAACAACAGTCCATCAGGAGTGTTCACATTTGGTGCAAATTCTAGCACAC CTGCAGCCTCAGCCCAGCCTTCAGGCTCGGGGGGCTTTCCATTTAACCAGTCTCCAGCAGCATTTACAGT GGGGTCAAATGGGAAAAATGTGTTCTCTTCTTCTGGAACTTCATTCTCTGGTCGCAAGATAAAGACTGCT GTTAGACGCAGGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208264 representing NM_005124
Red=Cloning site Green=Tags(s) MASGAGGVGGGGGGKIRTRRCHQGPIKPYQQGRQQHQGILSRVTESVKNIVPGWLQRYFNKNEDVCSCST DTSEVPRWPENKEDHLVYADEESSNITDGRITPEPAVSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSM LESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASDKDITVSKNTSLPPLWSP EAERSHSLSQHTATSSKKPAFNLSAFGTLSPSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQSKLRN TPYQAPVRRQMKAKQLSAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSIVSSPLNSPLDRSGIDITDF QAKREKVDSQYPPVQRLMTPKPVSIATNRSVYFKPSLTPSGEFRKTNQRIDNKCSTGYEKNMTPGQNREQ RESGFSYPNFSLPAANGLSSGVGGGGGKMRRERTRFVASKPLEEEEMEVPVLPKISLPITSSSLPTFNFS SPEITTSSPSPINSSQALTNKVQMTSPSSTGSPMFKFSSPIVKSTEANVLPPSSIGFTFSVPVAKTAELS GSSSTLEPIISSSAHHVTTVNSTNCKKTPPEDCEGPFRPAEILKEGSVLDILKSPGFASPKIDSVAAQPT ATSPVVYTRPAISSFSSSGIGFGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLSPRDTAKQTGIETP NKSGKTTLSASGTGFGDKFKPVIGTWDCDTCLVQNKPEAIKCVACETPKPGTCVKRALTLTVVSESAETM TASSSSCTVTTGTLGFGDKFKRPIGSWECSVCCVSNNAEDNKCVSCMSEKPGSSVPASSSSTVPVSLPSG GSLGLEKFKKPEGSWDCELCLVQNKADSTKCLACESAKPGTKSGFKGFDTSSSSSNSAASSSFKFGVSSS SSGPSQTLTSTGNFKFGDQGGFKIGVSSDSGSINPMSEGFKFSKPIGDFKFGVSSESKPEEVKKDSKNDN FKFGLSSGLSNPVSLTPFQFGVSNLGQEEKKEELPKSSSAGFSFGTGVINSTPAPANTIVTSENKSSFNL GTIETKSASVAPFTCKTSEAKKEEMPATKGGFSFGNVEPASLPSASVFVLGRTEEKQQEPVTSTSLVFGK KADNEEPKCQPVFSFGNSEQTKDENSSKSTFSFSMTKPSEKESEQPAKATFAFGAQTSTTADQGAAKPVF SFLNNSSSSSSTPATSAGGGIFGSSTSSSNPPVATFVFGQSSNPVSSSAFGNTAESSTSQSLLFSQDSKL ATTSSTGTAVTPFVFGPGASSNNTTTSGFGFGATTTSSSAGSSFVFGTGPSAPSASPAFGANQTPTFGQS QGASQPNPPGFGSISSSTALFPTGSQPAPPTFGTVSSSSQPPVFGQQPSQSAFGSGTTPNSSSAFQFGSS TTNFNFTNNSPSGVFTFGANSSTPAASAQPSGSGGFPFNQSPAAFTVGSNGKNVFSSSGTSFSGRKIKTA VRRRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005124 |
| ORF Size | 4425 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005124.2, NP_005115.2 |
| RefSeq Size | 5687 bp |
| RefSeq ORF | 4428 bp |
| Locus ID | 9972 |
| UniProt ID | P49790 |
| Domains | zf-RanBP |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| MW | 153.8 kDa |
| Gene Summary | Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208264L1 | Lenti-ORF clone of NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 17,536.00 |
|
| RC208264L2 | Lenti-ORF clone of NUP153 (mGFP-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RC208264L3 | Lenti-ORF clone of NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RC208264L4 | Lenti-ORF clone of NUP153 (mGFP-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RG208264 | NUP153 (tGFP-tagged) - Human nucleoporin 153kDa (NUP153) |
CNY 16,250.00 |
|
| SC116943 | NUP153 (untagged)-Human nucleoporin 153kDa (NUP153) |
CNY 15,144.00 |
