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NUP153 (NM_005124) Human Untagged Clone
CAT＃: SC116943
NUP153 (untagged)-Human nucleoporin 153kDa (NUP153)
CNY 15,144.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HNUP153; N153 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | Please inquire |
| ACCN | NM_005124 |
| Insert Size | 5600 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005124.2, NP_005115.2 |
| RefSeq Size | 5687 bp |
| RefSeq ORF | 4428 bp |
| Locus ID | 9972 |
| UniProt ID | P49790 |
| Domains | zf-RanBP |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Gene Summary | Nuclear pore complexes regulate the transport of macromolecules between the nucleus and cytoplasm. They are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. Nucleoporins are glycoproteins found in nuclear pores and contain characteristic pentapeptide XFXFG repeats as well as O-linked N-acetylglucosamine residues oriented towards the cytoplasm. The protein encoded by this gene has three distinct domains: a N-terminal region containing a pore targeting and an RNA-binding domain domain, a central region containing multiple zinc finger motifs, and a C-terminal region containing multiple XFXFG repeats. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Nucleoporin 153 links nuclear pore complex to chromatin architecture by mediating CTCF and cohesin binding
,Kadota, S;Ou, J;Shi, Y;Lee, JT;Sun, J;Yildirim, E;,
Nat Commun
,PubMed ID 32451376
[NUP153]
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Nucleoporin 153 links nuclear pore complex to chromatin architecture by mediating CTCF and cohesin binding at cis-regulatory elements and TAD boundaries
,Kadota, S;Ou, J;Shi, Y;Sun, J;Yildirim, E;,
bioRxiv
[NUP153]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208264 | NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 15,136.00 |
|
| RC208264L1 | Lenti-ORF clone of NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 17,536.00 |
|
| RC208264L2 | Lenti-ORF clone of NUP153 (mGFP-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RC208264L3 | Lenti-ORF clone of NUP153 (Myc-DDK-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RC208264L4 | Lenti-ORF clone of NUP153 (mGFP-tagged)-Human nucleoporin 153kDa (NUP153) |
CNY 16,630.00 |
|
| RG208264 | NUP153 (tGFP-tagged) - Human nucleoporin 153kDa (NUP153) |
CNY 16,250.00 |
