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KIAA0427 (CTIF) (NM_014772) Human Tagged ORF Clone
CAT＃: RC208324
- TrueORF®
CTIF (Myc-DDK-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1
ORF Plasmid: tGFP
"NM_014772" in other vectors (4)
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CNY 5,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm672; KIAA0427 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208324 representing NM_014772
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACTCCTCTGCAGCATCAGCCTCCTCGGAGGCAGGGAGCAGCCGCTCCCAGGAGATCGAGGAGC TGGAGCGCTTCATCGACAGCTACGTGCTGGAGTACCAGGTGCAGGGGCTGCTGGCTGACAAGACGGAGGG TGATGGCGAGAGCGAGAGGACCCAGTCCCACATCTCCCAGTGGACAGCGGACTGCAGCGAACCGCTGGAC AGCAGCTGTTCCTTCTCCCGAGGGCGAGCCCCCCCACAGCAGAATGGCAGCAAAGACAACTCTCTGGACA TGCTGGGCACGGACATCTGGGCGGCCAACACCTTCGATTCCTTCAGTGGTGCCACCTGGGACCTGCAGCC GGAAAAGCTGGACTTCACCCAGTTCCACCGCAAAGTCCGACACACGCCCAAGCAGCCCCTGCCACACATC GACCGCGAAGGGTGTGGCAAAGGGAAGCTGGAAGATGGGGATGGCATCAACCTGAATGACATCGAGAAGG TCCTTCCAGCCTGGCAGGGCTACCACCCGATGCCCCATGAAGTGGAGATCGCACACACCAAGAAGCTGTT CCGCAGGAGGAGAAATGATCGAAGGCGGCAGCAGAGACCTCCGGGGGGCAACAAGCCCCAACAGCATGGT GACCACCAGCCAGGCAGTGCCAAACACAACAGGGACCACCAGAAATCCTACCAGGGGGGCTCAGCACCCC ACCCCTCAGGGAGGCCCACTCACCATGGCTACAGCCAGAACCGGCGCTGGCACCATGGCAACATGAAGCA CCCACCAGGCGACAAGGGGGAGGCAGGCGCACACCGCAATGCCAAAGAGACCATGACCATCGAGAACCCA AAACTGGAGGACACTGCAGGGGACACCGGGCACAGCAGCCTTGAGGCCCCCCGCAGCCCTGACACCCTGG CCCCGGTGGCTTCTGAGCGGCTGCCCCCACAGCAGTCAGGGGGGCCAGAGGTTGAGACAAAACGTAAAGA CAGTATTCTTCCCGAGCGCATCGGGGAGCGGCCCAAAATTACCCTGCTCCAGTCTTCCAAAGACAGACTG CGGCGAAGGCTAAAGGAAAAGGATGAAGTGGCCGTGGAGACGACCACTCCCCAGCAGAACAAGATGGACA AGCTGATCGAGATCCTGAACAGCATGCGGAACAACAGCAGCGACGTGGACACCAAGCTCACCACCTTCAT GGAGGAGGCCCAGAACTCCACCAACTCCGAGGAGATGCTGGGCGAGATCGTGCGCACAATCTACCAGAAG GCTGTGTCCGACCGCAGCTTCGCCTTCACCGCTGCCAAGCTCTGCGACAAGATGGCGCTCTTTATGGTGG AGGGGACCAAGTTCCGGAGCCTGCTCCTCAACATGCTGCAGAAGGACTTCACGGTGCGCGAGGAGCTGCA GCAGCAGGACGTGGAGCGCTGGCTGGGCTTCATCACCTTCCTGTGCGAGGTCTTCGGCACCATGCGCAGC AGCACAGGCGAGCCCTTCCGTGTGCTCGTGTGCCCCATCTACACCTGCCTCAGGGAGCTCTTGCAATCTC AGGATGTGAAGGAAGATGCTGTCCTTTGCTGCTCTATGGAGCTGCAGAGTACAGGCCGGCTGCTGGAGGA ACAGCTGCCTGAGATGATGACAGAGCTCCTGGCCAGCGCACGGGACAAGATGCTGTGCCCCTCGGAGTCC ATGCTGACCCGGTCGCTGCTCCTAGAGGTCATCGAGCTCCACGCTAACAGCTGGAACCCTCTGACGCCCC CCATCACGCAGTACTACAACAGAACCATCCAGAAACTGACAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208324 representing NM_014772
Red=Cloning site Green=Tags(s) MENSSAASASSEAGSSRSQEIEELERFIDSYVLEYQVQGLLADKTEGDGESERTQSHISQWTADCSEPLD SSCSFSRGRAPPQQNGSKDNSLDMLGTDIWAANTFDSFSGATWDLQPEKLDFTQFHRKVRHTPKQPLPHI DREGCGKGKLEDGDGINLNDIEKVLPAWQGYHPMPHEVEIAHTKKLFRRRRNDRRRQQRPPGGNKPQQHG DHQPGSAKHNRDHQKSYQGGSAPHPSGRPTHHGYSQNRRWHHGNMKHPPGDKGEAGAHRNAKETMTIENP KLEDTAGDTGHSSLEAPRSPDTLAPVASERLPPQQSGGPEVETKRKDSILPERIGERPKITLLQSSKDRL RRRLKEKDEVAVETTTPQQNKMDKLIEILNSMRNNSSDVDTKLTTFMEEAQNSTNSEEMLGEIVRTIYQK AVSDRSFAFTAAKLCDKMALFMVEGTKFRSLLLNMLQKDFTVREELQQQDVERWLGFITFLCEVFGTMRS STGEPFRVLVCPIYTCLRELLQSQDVKEDAVLCCSMELQSTGRLLEEQLPEMMTELLASARDKMLCPSES MLTRSLLLEVIELHANSWNPLTPPITQYYNRTIQKLTA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014772 |
| ORF Size | 1794 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014772.3 |
| RefSeq Size | 5748 bp |
| RefSeq ORF | 1797 bp |
| Locus ID | 9811 |
| UniProt ID | O43310 |
| MW | 67.6 kDa |
| Gene Summary | CTIF is a component of the CBP80 (NCBP1; MIM 600469)/CBP20 (NCBP2; MIM 605133) translation initiation complex that binds cotranscriptionally to the cap end of nascent mRNA. The CBP80/CBP20 complex is involved in a simultaneous editing and translation step that recognizes premature termination codons (PTCs) in mRNAs and directs PTC-containing mRNAs toward nonsense-mediated decay (NMD). On mRNAs without PTCs, the CBP80/CBP20 complex is replaced with cytoplasmic mRNA cap-binding proteins, including EIF4G (MIM 600495), and steady-state translation of the mRNAs resumes in the cytoplasm (Kim et al., 2009 [PubMed 19648179]).[supplied by OMIM, Dec 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208324L3 | Lenti-ORF clone of CTIF (Myc-DDK-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 7,890.00 |
|
| RC208324L4 | Lenti-ORF clone of CTIF (mGFP-tagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 7,890.00 |
|
| RG208324 | CTIF (tGFP-tagged) - Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 6,560.00 |
|
| SC304169 | CTIF (untagged)-Human CBP80/20-dependent translation initiation factor (CTIF), transcript variant 1 |
CNY 7,220.00 |
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