PEX6 (NM_000287) Human Tagged ORF Clone

CAT#: RC208361

  • TrueORF®

PEX6 (Myc-DDK-tagged)-Human peroxisomal biogenesis factor 6 (PEX6)

ORF Plasmid: DDK tGFP



  "NM_000287" in other vectors (6)

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CNY 7,176.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal antibody to PEX6 (peroxisomal biogenesis factor 6)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HMLR2; PAF-2; PAF2; PBD4A; PDB4B; PXAAA1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208361 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCTGGCTGTCTTGCGGGTCCTGGAGCCCTTTCCGACCGAGACACCCCCGTTGGCAGTGCTGCTGC
CACCCGGGGGCCCGTGGCCGGCGGCGGAGCTGGGCCTGGTGCTGGCCCTGAGGCCTGCAGGGGAGAGCCC
GGCAGGGCCGGCGCTGCTGGTGGCAGCCCTGGAGGGGCCGGACGCGGGCACCGAAGAGCAGGGTCCCGGG
CCGCCGCAGCTACTGGTTAGCCGCGCGCTGCTGCGGCTCCTGGCACTGGGCTCCGGGGCCTGGGTGCGGG
CGCGGGCGGTGCGGCGGCCCCCGGCGCTAGGTTGGGCACTGCTTGGCACCTCGCTGGGGCCTGGGCTCGG
ACCGCGAGTCGGGCCGCTGCTGGTGAGGCGCGGAGAGACCCTCCCAGTGCCCGGACCGCGGGTGCTGGAG
ACGCGGCCGGCGTTGCAAGGGCTGCTGGGCCCAGGGACTCGGCTGGCTGTGACTGAGCTCCGCGGGCGGG
CCAGACTGTGTCCAGAGTCTGGGGACAGCAGTCGGCCCCCACCCCCGCCCGTGGTGTCCTCCTTTGCGGT
TTCTGGCACAGTGCGGCGACTCCAGGGAGTTCTGGGAGGGACTGGAGATTCACTAGGGGTGAGCCGGAGC
TGTCTCCGTGGCCTTGGCCTCTTCCAGGGCGAATGGGTGTGGGTGGCCCAGGCCAGAGAGTCATCGAACA
CTTCACAGCCGCACTTGGCTAGGGTGCAGGTCCTAGAACCTCGCTGGGACCTCTCTGATAGACTGGGACC
CGGCTCTGGACCGCTGGGAGAGCCCCTCGCTGACGGACTGGCGCTTGTCCCTGCCACTTTGGCTTTTAAT
CTTGGCTGTGACCCCCTGGAAATGGGAGAGCTCAGAATTCAGAGGTACTTGGAAGGCTCCATCGCCCCTG
AAGACAAAGGAAGCTGCTCATTGCTGCCTGGGCCTCCATTTGCCAGAGAGTTACACATCGAAATTGTGTC
TTCTCCCCACTACAGCACTAATGGAAATTATGACGGTGTTCTTTACCGGCACTTTCAGATACCCAGGGTA
GTCCAGGAAGGGGATGTTCTATGTGTGCCAACAATTGGGCAAGTAGAGATCCTGGAAGGAAGTCCAGAGA
AACTGCCCAGGTGGCGGGAAATGTTTTTTAAAGTGAAGAAAACAGTTGGGGAAGCTCCAGATGGACCAGC
CAGTGCCTACTTGGCCGACACCACCCATACCTCCTTGTACATGGTGGGTTCTACCCTGAGCCCTGTTCCA
TGGCTCCCTTCAGAGGAATCCACTCTCTGGAGCAGTTTGTCTCCTCCAGGCCTGGAGGCCTTGGTGTCTG
AACTCTGTGCTGTCCTGAAGCCTCGCCTCCAGCCAGGGGGTGCCCTGCTGACAGGAACTAGCAGTGTCCT
TCTACGGGGCCCCCCAGGCTGTGGGAAGACCACAGTAGTTGCTGCTGCCTGTAGTCACCTTGGGCTCCAC
TTACTGAAGGTGCCCTGCTCCAGCCTCTGTGCAGAAAGTAGTGGGGCTGTGGAGACAAAACTGCAGGCCA
TCTTCTCCCGGGCCCGCCGTTGCCGGCCTGCAGTCCTGTTGCTCACAGCTGTGGACCTTCTGGGCCGGGA
CCGTGATGGGCTGGGTGAGGATGCCCGTGTGATGGCTGTGCTGCGTCACCTCCTCCTCAATGAGGACCCC
CTCAACAGCTGCCCTCCCCTCATGGTTGTGGCCACCACAAGCCGGGCCCAGGACCTGCCTGCTGATGTGC
AGACAGCATTTCCTCATGAGCTCGAGGTGCCTGCTCTGTCAGAGGGGCAGCGGCTCAGCATCCTGCGGGC
CCTCACTGCCCACCTTCCCCTGGGCCAGGAGGTGAACTTGGCACAGCTAGCACGGCGGTGTGCAGGCTTT
GTGGTAGGGGATCTCTATGCCCTTCTGACCCACAGCAGCCGGGCAGCCTGCACCAGGATCAAGAACTCAG
GTTTGGCAGGTGGCTTGACTGAGGAGGATGAGGGGGAGCTGTGTGCTGCCGGCTTTCCTCTCCTGGCTGA
GGACTTTGGGCAGGCACTGGAGCAACTGCAGACAGCTCACTCCCAGGCCGTTGGAGCCCCCAAGATCCCC
TCAGTGTCCTGGCATGATGTGGGTGGGCTGCAGGAGGTGAAGAAGGAGATCCTGGAGACCATTCAGCTCC
CCCTGGAGCACCCTGAGCTACTGAGCCTGGGCCTGAGACGCTCAGGCCTTCTGCTCCATGGGCCCCCTGG
CACCGGCAAGACCCTTCTGGCCAAGGCAGTAGCCACTGAGTGCAGCCTTACCTTCCTCAGCGTGAAGGGG
CCAGAGCTCATTAACATGTATGTGGGCCAAAGTGAGGAGAATGTGCGGGAAGTATTTGCCAGGGCCAGGG
CTGCAGCTCCATGCATTATCTTCTTTGATGAACTGGACTCTTTGGCCCCAAGCCGGGGGCGAAGTGGAGA
TTCTGGAGGAGTGATGGACAGGGTGGTGTCTCAGCTCCTTGCCGAGCTAGATGGGCTGCACAGCACTCAG
GATGTGTTTGTGATTGGAGCCACCAACAGACCAGATCTCCTGGACCCTGCCCTTCTGCGGCCTGGCAGAT
TTGACAAGCTGGTGTTTGTGGGGGCAAATGAGGACCGGGCCTCCCAGCTACGCGTTCTAAGTGCCATCAC
ACGCAAATTCAAGCTAGAGCCATCTGTGAGCCTGGTAAACGTGCTAGATTGCTGCCCTCCCCAGCTGACG
GGCGCGGACCTCTACTCTCTCTGCTCTGATGCTATGACAGCTGCCCTCAAACGCAGGGTTCATGACCTGG
AGGAAGGGCTGGAGCCAGGTAGCTCAGCACTGATGCTCACCATGGAGGACTTGCTGCAGGCTGCCGCCCG
GCTGCAACCCTCAGTCAGTGAGCAGGAGCTGCTCCGGTACAAGCGCATCCAGCGCAAGTTTGCTGCCTGC


ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAG
GTTTAA
>RC208361 protein sequence
Red=Cloning site Green=Tags(s)

MALAVLRVLEPFPTETPPLAVLLPPGGPWPAAELGLVLALRPAGESPAGPALLVAALEGPDAGTEEQGPG
PPQLLVSRALLRLLALGSGAWVRARAVRRPPALGWALLGTSLGPGLGPRVGPLLVRRGETLPVPGPRVLE
TRPALQGLLGPGTRLAVTELRGRARLCPESGDSSRPPPPPVVSSFAVSGTVRRLQGVLGGTGDSLGVSRS
CLRGLGLFQGEWVWVAQARESSNTSQPHLARVQVLEPRWDLSDRLGPGSGPLGEPLADGLALVPATLAFN
LGCDPLEMGELRIQRYLEGSIAPEDKGSCSLLPGPPFARELHIEIVSSPHYSTNGNYDGVLYRHFQIPRV
VQEGDVLCVPTIGQVEILEGSPEKLPRWREMFFKVKKTVGEAPDGPASAYLADTTHTSLYMVGSTLSPVP
WLPSEESTLWSSLSPPGLEALVSELCAVLKPRLQPGGALLTGTSSVLLRGPPGCGKTTVVAAACSHLGLH
LLKVPCSSLCAESSGAVETKLQAIFSRARRCRPAVLLLTAVDLLGRDRDGLGEDARVMAVLRHLLLNEDP
LNSCPPLMVVATTSRAQDLPADVQTAFPHELEVPALSEGQRLSILRALTAHLPLGQEVNLAQLARRCAGF
VVGDLYALLTHSSRAACTRIKNSGLAGGLTEEDEGELCAAGFPLLAEDFGQALEQLQTAHSQAVGAPKIP
SVSWHDVGGLQEVKKEILETIQLPLEHPELLSLGLRRSGLLLHGPPGTGKTLLAKAVATECSLTFLSVKG
PELINMYVGQSEENVREVFARARAAAPCIIFFDELDSLAPSRGRSGDSGGVMDRVVSQLLAELDGLHSTQ
DVFVIGATNRPDLLDPALLRPGRFDKLVFVGANEDRASQLRVLSAITRKFKLEPSVSLVNVLDCCPPQLT
GADLYSLCSDAMTAALKRRVHDLEEGLEPGSSALMLTMEDLLQAAARLQPSVSEQELLRYKRIQRKFAAC

TRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-NotI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000287
ORF Size 2940 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000287.4
RefSeq Size 3514 bp
RefSeq ORF 2943 bp
Locus ID 5190
UniProt ID Q13608
Domains AAA, AAA
Protein Families Druggable Genome
MW 104.1 kDa
Gene Summary This gene encodes a member of the AAA (ATPases associated with diverse cellular activities) family of ATPases. This member is a predominantly cytoplasmic protein, which plays a direct role in peroxisomal protein import and is required for PTS1 (peroxisomal targeting signal 1, a C-terminal tripeptide of the sequence ser-lys-leu) receptor activity. Mutations in this gene cause peroxisome biogenesis disorders of complementation group 4 and complementation group 6. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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