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SREBP1 (SREBF1) (NM_004176) Human Tagged ORF Clone
CAT＃: RC208404
SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2
ORF Plasmid: tGFP
"NM_004176" in other vectors (6)
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CNY 11,976.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | bHLHd1; HMD; IFAP2; SREBP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208404 representing NM_004176
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGACGAGCCACCCTTCAGCGAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGG CGCTGCTGACCGACATCGAAGACATGCTTCAGCTTATCAACAACCAAGACAGTGACTTCCCTGGCCTATT TGACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGCTCCCCAGGC AGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTGAGCGGGCCGCAGGCAGCGC CCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTGAAGATGTACCCGTCCATGCCCGCTTTCTC CCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACTGAGCATCCTGCAGACCCCCACCCCACAGCCCCTG CCAGGGGCCCTCCTGCCACAGAGCTTCCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAG GCTACCCCAGCCCTCCGGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAACACCCAGCAGCCGCTGCCTGG CCTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCCAGGTCCAGAGTGTGGTCCCC CAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGACCACTGTGACCTCGCAGATCC AGCAGGTCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCAGACTCGCTGCTTCTGACAGCCATGAAGAC AGACGGAGCCACTGTGAAGGCGGCAGGTCTCAGTCCCCTGGTCTCTGGCACCACTGTGCAGACAGGGCCT TTGCCGACCCTGGTGAGTGGCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTGC CTATCAACCGGCTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGCCGTGGAGAGAAGCGCACAGC CCACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAATCATTGAGCTCAAGGATCTGGTG GTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCATCGACTACATTCGCTTTCTGC AACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTGCGCACTGCTGTCCACAAAAGCAAATCTCT GAAGGATCTGGTGTCGGCCTGTGGCAGTGGAGGGAACACAGACGTGCTCATGGAGGGCGTGAAGACTGAG GTGGAGGACACACTGACCCCACCCCCCTCGGATGCTGGCTCACCTTTCCAGAGCAGCCCCTTGTCCCTTG GCAGAAGGGGCAGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGACAGCCCAGTCTTTGAGGACAG CAAGGCAAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGGACCGCTCCCGCCTGGCCCTG TGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCTTGCTGGGGGCCCGGGGGCTTCCCA GCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGCAACGTGCTGGGCACCGAGAGCAGAGATGG CCCTGGCTGGGCCCAGTGGCTGCTGCCCCCAGTGGTCTGGCTGCTCAATGGGCTGTTGGTGCTCGTCTCC TTGGTGCTTCTCTTTGTCTACGGTGAGCCAGTCACACGGCCCCACTCAGGCCCCGCCGTGTACTTCTGGA GGCATCGCAAGCAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAGGCTGCCCAGCAGCTGTGGCT GGCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGGCTTGTAGCCTCCTCTGGAAC CTCATTCGTCACCTGCTGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGGCCGGGCAGGGGGCCTGCAGC AGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGAGACGCAGCCCTGGTCTACCATAAGCTGCA CCAGCTGCACACCATGGGGAAGCACACAGGCGGGCACCTCACTGCCACCAACCTGGCGCTGAGTGCCCTG AACCTGGCAGAGTGTGCAGGGGATGCCGTGTCTGTGGCGACGCTGGCCGAGATCTATGTGGCGGCTGCAT TGAGAGTGAAGACCAGTCTCCCACGGGCCTTGCATTTTCTGACACGCTTCTTCCTGAGCAGTGCCCGCCA GGCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCTGCCACCCCGTGGGCCACCGT TTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCTGTACAGCTTGGCCGGGAACC CAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCCGGGAACATCTCTTAGAGCGAGCACTGAACTGTGT GACCCAGCCCAACCCCAGCCCTGGGTCAGCTGATGGGGACAAGGAATTCTCGGATGCCCTCGGGTACCTG CAGCTGCTGAACAGCTGTTCTGATGCTGCGGGGGCTCCTGCCTACAGCTTCTCCATCAGTTCCAGCATGG CCACCACCACCGGCGTAGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACAGCTGTGGTGATCCACTGGCT GCGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACCTGCCCCGGGTGCTGCAGGAG TCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCGGGCCCTGCTGGGCTGTGCCA AGGCAGAGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAGGCCAGTGGGTACCTGCAGGACAGCCTGGC TACCACACCAGCCAGCAGCTCCATTGACAAGGCCGTGCAGCTGTTCCTGTGTGACCTGCTTCTTGTGGTG CGCACCAGCCTGTGGCGGCAGCAGCAGCCCCCGGCCCCGGCCCCAGCAGCCCAGGGCACCAGCAGCAGGC CCCAGGCTTCCGCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGCCTGAGGCGGCTGGCACAGAG CTTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGAGGCCACGGCCCGGCTGATGGCGGGGGCCAGCCCC ACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCCCGGTGGCAAAGGAGGCGCGG TGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCGGAGGCCTTGCTGCTGGCCTCCTGCTACCT GCCCCCCGGCTTCCTGTCGGCGCCCGGGCAGCGCGTGGGCATGCTGGCTGAGGCGGCGCGCACACTCGAG AAGCTTGGCGATCGCCGGCTGCTGCACGACTGTCAGCAGATGCTCATGCGCCTGGGCGGTGGGACCACTG TCACTTCCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208404 representing NM_004176
Red=Cloning site Green=Tags(s) MDEPPFSEAALEQALGEPCDLDAALLTDIEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPG SLSPPPATLSSSLEAFLSGPQAAPSPLSPPQPAPTPLKMYPSMPAFSPGPGIKEESVPLSILQTPTPQPL PGALLPQSFPAPAPPQFSSTPVLGYPSPPGGFSTGSPPGNTQQPLPGLPLASPPGVPPVSLHTQVQSVVP QQLLTVTAAPTAAPVTTTVTSQIQQVPVLLQPHFIKADSLLLTAMKTDGATVKAAGLSPLVSGTTVQTGP LPTLVSGGTILATVPLVVDAEKLPINRLAAGSKAPASAQSRGEKRTAHNAIEKRYRSSINDKIIELKDLV VGTEAKLNKSAVLRKAIDYIRFLQHSNQKLKQENLSLRTAVHKSKSLKDLVSACGSGGNTDVLMEGVKTE VEDTLTPPPSDAGSPFQSSPLSLGRRGSGSGGSGSDSEPDSPVFEDSKAKPEQRPSLHSRGMLDRSRLAL CTLVFLCLSCNPLASLLGARGLPSPSDTTSVYHSPGRNVLGTESRDGPGWAQWLLPPVVWLLNGLLVLVS LVLLFVYGEPVTRPHSGPAVYFWRHRKQADLDLARGDFAQAAQQLWLALRALGRPLPTSHLDLACSLLWN LIRHLLQRLWVGRWLAGRAGGLQQDCALRVDASASARDAALVYHKLHQLHTMGKHTGGHLTATNLALSAL NLAECAGDAVSVATLAEIYVAAALRVKTSLPRALHFLTRFFLSSARQACLAQSGSVPPAMQWLCHPVGHR FFVDGDWSVLSTPWESLYSLAGNPVDPLAQVTQLFREHLLERALNCVTQPNPSPGSADGDKEFSDALGYL QLLNSCSDAAGAPAYSFSISSSMATTTGVDPVAKWWASLTAVVIHWLRRDEEAAERLCPLVEHLPRVLQE SERPLPRAALHSFKAARALLGCAKAESGPASLTICEKASGYLQDSLATTPASSSIDKAVQLFLCDLLLVV RTSLWRQQQPPAPAPAAQGTSSRPQASALELRGFQRDLSSLRRLAQSFRPAMRRVFLHEATARLMAGASP TRTHQLLDRSLRRRAGPGGKGGAVAELEPRPTRREHAEALLLASCYLPPGFLSAPGQRVGMLAEAARTLE KLGDRRLLHDCQQMLMRLGGGTTVTSS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004176 |
| ORF Size | 3441 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004176.4 |
| RefSeq Size | 4922 bp |
| RefSeq ORF | 3444 bp |
| Locus ID | 6720 |
| UniProt ID | P36956 |
| Domains | HLH |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Insulin signaling pathway |
| MW | 122.2 kDa |
| Gene Summary | This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Mutations in SREBF1, Encoding Sterol Regulatory Element Binding Transcription Factor 1, Cause Autosomal-Dominant IFAP Syndrome
,Wang, H;Humbatova, A;Liu, Y;Qin, W;Lee, M;Cesarato, N;Kortüm, F;Kumar, S;Romano, M;Dai, S;Mo, R;Sivalingam, S;Motameny, S;Wu, Y;Wang, X;Niu, X;Geng, S;Bornholdt, D;Kroisel, P;Tadini, G;Walter, S;Hauck, F;Girisha, K;Calza, A;Bottani, A;Altmüller, J;Buness, A;Yang, S;Sun, X;Ma, L;Kutsche, K;Grzeschik, K;Betz, R;Lin, Z;,
The American Journal of Human Genetics
,PubMed ID 32497488
[SREBP1]
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|
Cholesterol-Independent SREBP-1 Maturation Is Linked to ARF1 Inactivation
,Smulan, LJ;Ding, W;Freinkman, E;Gujja, S;Edwards, YJ;Walker, AK;,
Cell Rep
,PubMed ID 27320911
[SREBP1]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208404L1 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RC208404L2 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 13,300.00 |
|
| RC208404L3 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RC208404L4 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RG208404 | SREBF1 (tGFP-tagged) - Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 13,576.00 |
|
| SC117502 | SREBF1 (untagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 11,992.00 |
