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SREBP1 (SREBF1) (NM_004176) Human Untagged Clone
CAT＃: SC117502
SREBF1 (untagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2
CNY 11,992.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | bHLHd1; HMD; IFAP2; SREBP1 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004176 edited
GGCGGCGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGC GGCGGGCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGACGAGCCACCCTTCAGC GAGGCGGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACC GACATCGAAGACATGCTTCAGCTTATCAACAACCAAGATAGTGACTTCCCTGGCCTATTT GACCCACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGC TCCCCAGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTG AGCGGGCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTG AAGATGTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTG CCACTGAGCATCCTGCAGACCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAG AGCTTCCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGC CCTCCGGGAGGCTTCTCTACAGGAAGCCCTCCCGGGAACACCCAGCAGCCGCTGCCTGGC CTGCCACTGGCTTCCCCGCCAGGGGTCCCGCCCGTCTCCTTGCACACCCAGGTCCAGAGT GTGGTCCCCCAGCAGCTACTGACAGTCACAGCTGCCCCCACGGCAGCCCCTGTAACGACC ACTGTGACCTCGCAGATCCAGCAGGTCCCGGTCCTGCTGCAGCCCCACTTCATCAAGGCA GACTCGCTGCTTCTGACAGCCATGAAGACAGATGGAGCCACTGTGAAGGCGGCAGGTCTC AGTCCCCTGGTCTCTGGCACCACTGTGCAGACAGGGCCTTTGCCGACCCTGGTGAGTGGC GGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTGCCTATCAACCGG CTCGCAGCTGGCAGCAAGGCCCCGGCCTCTGCCCAGAGCCGTGGAGAGAAGCGCACAGCC CACAACGCCATTGAGAAGCGCTACCGCTCCTCCATCAATGACAAAATCATTGAGCTCAAG GATCTGGTGGTGGGCACTGAGGCAAAGCTGAATAAATCTGCTGTCTTGCGCAAGGCCATC GACTACATTCGCTTTCTGCAACACAGCAACCAGAAACTCAAGCAGGAGAACCTAAGTCTG CGCACTGCTGTCCACAAAAGCAAATCTCTGAAGGATCTGGTGTCGGCCTGTGGCAGTGGA GGGAACACAGACGTGCTCATGGAGGGCGTGAAGACTGAGGTGGAGGACACACTGACCCCA CCCCCCTCGGATGCTGGCTCACCTTTCCAGAGCAGCCCCTTGTCCCTTGGCAGCAGGGGC AGTGGCAGCGGTGGCAGTGGCAGTGACTCGGAGCCTGACAGCCCAGTCTTTGAGGACAGC AAGGCAAAGCCAGAGCAGCGGCCGTCTCTGCACAGCCGGGGCATGCTGGACCGCTCCCGC CTGGCCCTGTGCACGCTCGTCTTCCTCTGCCTGTCCTGCAACCCCTTGGCCTCCTTGCTG GGGGCCCGGGGGCTTCCCAGCCCCTCAGATACCACCAGCGTCTACCATAGCCCTGGGCGC AACGTGCTGGGCACCGAGAGCAGAGATGGCCCTGGCTGGGCCCAGTGGCTGCTGCCCCCA GTGGTCTGGCTGCTCAATGGGCTGTTGGTGCTCGTCTCCTTGGTGCTTCTCTTTGTCTAC GGTGAGCCAGTCACACGGCCCCACTCAGGCCCCGCCGTGTACTTCTGGAGGCATCGCAAG CAGGCTGACCTGGACCTGGCCCGGGGAGACTTTGCCCAGGCTGCCCAGCAGCTGTGGCTG GCCCTGCGGGCACTGGGCCGGCCCCTGCCCACCTCCCACCTGGACCTGGCTTGTAGCCTC CTCTGGAACCTCATCCGTCACCTGCTGCAGCGTCTCTGGGTGGGCCGCTGGCTGGCAGGC CGGGCAGGGGGCCTGCAGCAGGACTGTGCTCTGCGAGTGGATGCTAGCGCCAGCGCCCGA GACGCAGCCCTGGTCTACCATAAGCTGCACCAGCTGCACACCATGGGGAAGCACACAGGC GGGCACCTCACTGCCACCAACCTGGCGCTGAGTGCCCTGAACCTGGCAGAGTGTGCAGGG GATGCCGTGTCTGTGGCGACGCTGGCCGAGATCTATGTGGCGGCTGCATTGAGAGTGAAG ACCAGTCTCCCACGGGCCTTGCATTTTCTGACACGCTTCTTCCTGAGCAGTGCCCGCCAG GCCTGCCTGGCACAGAGTGGCTCAGTGCCTCCTGCCATGCAGTGGCTCTGCCACCCCGTG GGCCACCGTTTCTTCGTGGATGGGGACTGGTCCGTGCTCAGTACCCCATGGGAGAGCCTG TACAGCTTGGCCGGGAACCCAGTGGACCCCCTGGCCCAGGTGACTCAGCTATTCCGGGAA CATCTCTTAGAGCGAGCACTGAACTGTGTGACCCAGCCCAACCCCAGCCCTGGGTCAGCT GATGGGGACAAGGAATTCTCGGATGCCCTCGGGTACCTGCAGCTGCTGAACAGCTGTTCT GATGCTGCGGGGGCTCCTGCCTACAGCTTCTCCATCAGTTCCAGCATGGCCACCACCACC GGCGTAGACCCGGTGGCCAAGTGGTGGGCCTCTCTGACAGCTGTGGTGATCCACTGGCTG CGGCGGGATGAGGAGGCGGCTGAGCGGCTGTGCCCGCTGGTGGAGCACCTGCCCCGGGTG CTGCAGGAGTCTGAGAGACCCCTGCCCAGGGCAGCTCTGCACTCCTTCAAGGCTGCCCGG GCCCTGCTGGGCTGTGCCAAGGCAGAGTCTGGTCCAGCCAGCCTGACCATCTGTGAGAAG GCCAGTGGGTACCTGCAGGACAGCCTGGCTACCACACCAGCCAGCAGCTCCATTGACAAG GCCGTGCAGCTGTTCCTGTGTGACCTGCTTCTTGTGGTGCGCACCAGCCTGTGGCGGCAG CAGCAGCCCCCGGCCCCGGCCCCAGCAGCCCAGGGCACCAGCAGCAGGCCCCAGGCTTCC GCCCTTGAGCTGCGTGGCTTCCAACGGGACCTGAGCAGCCTGAGGCGGCTGGCACAGAGC TTCCGGCCCGCCATGCGGAGGGTGTTCCTACATGAGGCCACGGCCCGGCTGATGGCGGGG GCCAGCCCCACACGGACACACCAGCTCCTCGACCGCAGTCTGAGGCGGCGGGCAGGCCCC GGTGGCAAAGGAGGCGCGGTGGCGGAGCTGGAGCCGCGGCCCACGCGGCGGGAGCACGCG GAGGCCTTGCTGCTGGCCTCCTGCTACCTGCCCCCCGGCTTCCTGTCGGCGCCCGGGCAG CGCGTGGGCATGCTGGCTGAGGCGGCGCGCACACTCGAGAAGCTTGGCGATCGCCGGCTG CTGCACGACTGTCAGCAGATGCTCATGCGCCTGGGCGGTGGGACCACTGTCACTTCCAGC TAGACCCCGTGTCCCCGGCCTCAGCACCCCTGTCTCTAGCCACTTTGGTCCCGTGCAGCT TCTGTCCTGCGTCGAAGCTTTGAAGGCCGAAGGCAGTGCAAGA >OriGene 5' read for NM_004176 unedited
CGAATTCACCGCCCGTTGNCGCATTGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGCCGCCTCTGAGGCCAGGGCAGGACACGAACGCGCGGAGCGGCGG CGGCGACTGAGAGCCGGGGCCGCGGCGGCGCTCCCTAGGAAGGGCCGTACGAGGCGGCGG GCCCGGCGGGCCTCCCGGAGGAGGCGGCTGCGCCATGGACGAGCCACCCTTCAGCGAGGC GGCTTTGGAGCAGGCGCTGGGCGAGCCGTGCGATCTGGACGCGGCGCTGCTGACCGACAT CGAAGACATGCTTCAGCTTATCAACAACCAAGATAGTGACTTCCCTGGCCTATTTGACCC ACCCTATGCTGGGAGTGGGGCAGGGGGCACAGACCCTGCCAGCCCCGATACCAGCTCCCC AGGCAGCTTGTCTCCACCTCCTGCCACATTGAGCTCCTCTCTTGAAGCCTTCCTGAGCGG GCCGCAGGCAGCGCCCTCACCCCTGTCCCCTCCCCAGCCTGCACCCACTCCATTGAAGAT GTACCCGTCCATGCCCGCTTTCTCCCCTGGGCCTGGTATCAAGGAAGAGTCAGTGCCACT GAGCATCCTGCAGACCCCCACCCCACAGCCCCTGCCAGGGGCCCTCCTGCCACAGAGCTT NCCAGCCCCAGCCCCACCGCAGTTCAGCTCCACCCCTGTGTTAGGCTACCCCAGCCCTCC GGGAGGCTTCTCTACAGGAAGCCCCTCCGGGAACACCCAGCAGCCGCTGCCTGGCCTGCC ACTGGCTTCCCGNNCCAGGGTCCCGCCCGTCTCCTTGCCACCCAGTCCANAGAAGGGTCC CCCAGCAGC >OriGene 3' read for NM_004176 unedited
NCGACGGAAATACTATGTACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAATTTCATTTTTAAT TCTCTGGACAAAACTTCTCAATCTATGAAAATAAAGTTTGCAAAAGGCAAAGTACCCCAG GAGCCTCAGGTCAGGAGGCTAAACCCCCTGACCTACACTATGTACACGTTTCTCTCCCAC AAAGGAAAAAAAGGCCTCTGGGGCAAAACCCTGCTTGCAGTCCGGGAAAACCCAATTGGC TTCCGTCAGCCCAGGGAAATGTACCCCTCTTTTCCCTGGTCCCCGGGGGAAAAAAGCCCC TTAGGGGCCCGCAAAACAAGGAGGGGGGCCCCCCAAAATGGGTCGGCCCCTGGAGTGCCC CGATTGGCCCCCCAAGGTCAACCCCCTTTTGGCCCCCCGTCTTAAGGTCAAAAATGCGCC CCTTGGGCCCTCCACCGGGAAAGCCCCCAACAACCCCCGGTCCAACTGGGGGGGCCCAAA TTTTTTGGGCTGGCTTTGGCCTTTAAAGCTTTCACCCCAGACAAAAACCGCCCCGGACCC AAATGGCTTTAAACAGGGGTGCCTTAAGCCCGGGACCCCGGGTCCAACCGGGAAATGACA AAGGGGCCCCCCCCCCCAGCCCAATGAACATTTTGTTGAAAATTCTGGCAAAAACCCGGG ATTCCCCCAACTTTTTTGAGGTGGGCCCCCCCCCCCTTAACCAAATATCCCCACCCCCTT TCCCGGGCCCCCAAAAGAAAACCCGGGGGCCGGTTTCAAGGAGGGCCCACTACAAGGCCC CCCCGGGGCTCCCCCCCCGGGGGGCCGGGGTTTCAATTTTCCCACCCGCCCCCCTTTGTC C |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004176 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004176.3, NP_004167.3 |
| RefSeq Size | 4194 bp |
| RefSeq ORF | 3444 bp |
| Locus ID | 6720 |
| UniProt ID | P36956 |
| Domains | HLH |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | Insulin signaling pathway |
| Gene Summary | This gene encodes a basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a motif that is found in the promoter of the low density lipoprotein receptor gene and other genes involved in sterol biosynthesis. The encoded protein is synthesized as a precursor that is initially attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription. This cleaveage is inhibited by sterols. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternative promoter usage and splicing result in multiple transcript variants, including SREBP-1a and SREBP-1c, which correspond to RefSeq transcript variants 2 and 3, respectively. [provided by RefSeq, Nov 2017] Transcript Variant: This variant (2, also known as SREBP-1a) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b, or SREBP-1a) has the same N- and C-termini but is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Activation of Androgen Receptor, Lipogenesis, and Oxidative Stress Converged by SREBP-1 Is Responsible for Regulating Growth and Progression of Prostate Cancer Cells
,Wen-Chin Huang, Xiangyan Li, Jian Liu, Jentai Lin, and Leland W.K. Chung,
Mol. Cancer Res., Jan 2012; 10: 133 - 142.
[SREBF1]
|
|
Systems biology of the autophagy-lysosomal pathway
,null,
Autophagy
,PubMed ID 21293178
[SREBF1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208404 | SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 11,976.00 |
|
| RC208404L1 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RC208404L2 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 13,300.00 |
|
| RC208404L3 | Lenti-ORF clone of SREBF1 (Myc-DDK-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RC208404L4 | Lenti-ORF clone of SREBF1 (mGFP-tagged)-Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 14,376.00 |
|
| RG208404 | SREBF1 (tGFP-tagged) - Human sterol regulatory element binding transcription factor 1 (SREBF1), transcript variant 2 |
CNY 13,576.00 |
