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ERO1LB (ERO1B) (NM_019891) Human Tagged ORF Clone
CAT＃: RC208445
ERO1LB (Myc-DDK-tagged)-Human ERO1-like beta (S. cerevisiae) (ERO1LB)
ORF Plasmid: tGFP
"NM_019891" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Ero1beta; ERO1LB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208445 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCAAGGGGTCCGCCGGGCAGGCGCTGGGCAGGGGGTAGCGGCCGCGGTGCAGCTGCTGGTCACCC TGAGCTTCCTGCGGAGCGTCGTCGAGGCGCAGGTCACTGGAGTTCTGGATGATTGCTTGTGTGATATTGA CAGCATCGATAACTTCAATACCTACAAAATCTTCCCCAAAATAAAAAAATTGCAAGAGAGAGACTATTTT CGTTATTACAAGGTTAATCTGAAGCGACCTTGTCCTTTCTGGGCAGAAGATGGCCACTGTTCAATAAAAG ACTGTCATGTGGAGCCCTGTCCAGAGAGTAAAATTCCGGTTGGAATAAAAGCTGGGCATTCTAATAAGTA CTTGAAAATGGCAAACAATACCAAAGAATTAGAAGTTTGTGAGCAAGCTAATAAACTGGGAGCAATTAAC AGCACATTAAGTAATCAAAGCAAAGAAGCTTTCATTGACTGGGCAAGATATGATGATTCACGGGATCACT TTTGTGAACTTGATGATGAGAGATCTCCAGCTGCTCAGTATGTAGACCTATTGCTGAACCCAGAGCGTTA CACTGGCTATAAAGGGACCTCTGCATGGAGAGTGTGGAACAGCATCTATGAAGAGAACTGTTTCAAGCCT CGATCTGTTTATCGTCCTTTAAATCCTCTGGCGCCTAGCCGAGGCGAAGATGATGGAGAATCATTCTACA CATGGCTAGAAGGTTTGTGTCTGGAGAAAAGAGTCTTCTATAAGCTTATATCGGGACTTCATGCTAGCAT CAATTTACATCTATGCGCAAATTATCTTTTGGAAGAAACCTGGGGTAAGCCCAGTTGGGGACCTAATATT AAAGAATTCAAACACCGCTTTGACCCTGTGGAAACCAAGGGAGAAGGTCCAAGAAGGCTCAAGAATCTTT ACTTTTTATACTTGATTGAGCTTCGAGCTTTGTCAAAGGTGGCTCCATATTTTGAGCGCTCAATTGTCGA TCTTTACACTGGAAATGCAGAAGAAGATGCTGACACAAAAACTCTTCTACTGAATATCTTTCAAGATACA AAGTCCTTTCCCATGCACTTTGATGAGAAATCCATGTTTGCAGGTGACAAAAAAGGGGCCAAGTCACTAA AGGAGGAATTCCGATTACATTTCAAGAATATCTCCCGTATAATGGACTGTGTTGGATGTGACAAATGCAG ATTATGGGGAAAATTACAGACTCAGGGTTTAGGAACTGCCCTGAAGATATTATTCTCTGAAAAAGAAATC CAAAAGCTTCCAGAGAATAGTCCATCTAAAGGCTTCCAACTCACCCGACAGGAAATAGTTGCTCTTTTAA ATGCTTTTGGAAGGCTTTCTACAAGTATAAGAGACTTACAGAATTTTAAAGTCTTATTACAACACAGTAG G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208445 protein sequence
Red=Cloning site Green=Tags(s) MSQGVRRAGAGQGVAAAVQLLVTLSFLRSVVEAQVTGVLDDCLCDIDSIDNFNTYKIFPKIKKLQERDYF RYYKVNLKRPCPFWAEDGHCSIKDCHVEPCPESKIPVGIKAGHSNKYLKMANNTKELEVCEQANKLGAIN STLSNQSKEAFIDWARYDDSRDHFCELDDERSPAAQYVDLLLNPERYTGYKGTSAWRVWNSIYEENCFKP RSVYRPLNPLAPSRGEDDGESFYTWLEGLCLEKRVFYKLISGLHASINLHLCANYLLEETWGKPSWGPNI KEFKHRFDPVETKGEGPRRLKNLYFLYLIELRALSKVAPYFERSIVDLYTGNAEEDADTKTLLLNIFQDT KSFPMHFDEKSMFAGDKKGAKSLKEEFRLHFKNISRIMDCVGCDKCRLWGKLQTQGLGTALKILFSEKEI QKLPENSPSKGFQLTRQEIVALLNAFGRLSTSIRDLQNFKVLLQHSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019891 |
| ORF Size | 1401 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019891.2, NP_063944.2 |
| RefSeq Size | 5070 bp |
| RefSeq ORF | 1404 bp |
| Locus ID | 56605 |
| UniProt ID | Q86YB8 |
| Domains | ERO1 |
| MW | 53.5 kDa |
| Gene Summary | Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Other protein disulfide isomerase family members can also be reoxidized, but at lower rates compared to P4HB, including PDIA2 (50% of P4HB reoxidation rate), as well as PDIA3, PDIA4, PDIA6 and NXNDC12 (<10%). Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. May be involved in oxidative proinsulin folding in pancreatic cells, hence may play a role in glucose homeostasis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208445L1 | Lenti ORF clone of Human ERO1-like beta (S. cerevisiae) (ERO1LB), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208445L2 | Lenti ORF clone of Human ERO1-like beta (S. cerevisiae) (ERO1LB), mGFP tagged |
CNY 5,890.00 |
|
| RC208445L3 | Lenti ORF clone of Human ERO1-like beta (S. cerevisiae) (ERO1LB), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208445L4 | Lenti ORF clone of Human ERO1-like beta (S. cerevisiae) (ERO1LB), mGFP tagged |
CNY 5,890.00 |
|
| RG208445 | ERO1LB (tGFP-tagged) - Human ERO1-like beta (S. cerevisiae) (ERO1LB) |
CNY 5,256.00 |
|
| SC128104 | ERO1LB (untagged)-Human ERO1-like beta (S. cerevisiae) (ERO1LB) |
CNY 3,656.00 |
