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PARG (NM_003631) Human Tagged ORF Clone
CAT＃: RC208530
PARG (Myc-DDK-tagged)-Human poly (ADP-ribose) glycohydrolase (PARG)
ORF Plasmid: tGFP
"NM_003631" in other vectors (7)
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CNY 7,152.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PARG99 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208530 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACAACTTCGCCGG CTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGACCCCAAGGACGCTCACGTGCA GTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGGCGGGCGGGACAGCACAGAGGCAGCGCCACC TCGCTTGTTTTCAAACAAAAGACTATTACCAGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAG AAAGTTTGGATAGTAAAGAAAACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAA CTTTTACCAACATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAA AAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAAGGGAAACACA CGGAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAGCAGTTTAGTAATGCTAACAT TGATCGGTCACCTCAAAATGATGATCACAGTGACACAGATAGTGAAGAGAATAGAGACAATCAACAGTTT CTCACAACTGTAAAGCTTGCAAATGCAAAGCAGACTACAGAAGATGAACAGGCCAGAGAAGCCAAAAGCC ACCAGAAGTGCAGCAAGTCTTGCGATCCTGGGGAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGT GGTGCCAGAGAGTCCATTGTCAGATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAA TTGACTAGACAAGAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCCGAGTCACCGA TGGATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAGGAGACAAGTCCAGGTTTTGA TGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAACCTTCAAGGTTCCAAGCAAGAGACGCTGAC ATTGAATTTAGGAAACGGTACTCTACTAAGGGCGGTGAAGTTAGATTACATTTCCAATTTGAAGGAGGAG AGAGTCGCACTGGAATGAATGATTTAAATGCTAAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATG CAGAAATTCTAAGCAACATGGAAAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCA GAGGACAGAAGAAAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCTAAATACGTTC CACCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAGATGAGAAGAATGCCTCGGTG TGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCACACAGTAACTATTCGGGTAGATCTTTTGCGA GCAGGAGAAGTTCCTAAACCTTTTCCAACACATTATAAAGATTTGTGGGATAACAAGCATGTTAAAATGC CTTGTTCAGAACAAAATTTGTACCCAGTGGAAGATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGA GCTCATTCAGACTGCACTTCTCAACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATAC AATGTGGCATATTCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTACTTGAAGAAG CAGAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATTGCACTCTGTCTGCCAAATAT TTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAATCATTCCATCACAATGTCGCAGGAACAGATT GCCAGTCTTTTAGCTAATGCTTTCTTCTGCACATTTCCACGACGAAATGCTAAGATGAAATCGGAGTATT CTAGTTACCCAGACATTAACTTCAATCGATTGTTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAA AACGCTCTTCTGCTACTTTAGAAGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAG AGTCTTGAAGATTTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTCACTTACGAAG GTACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGAGGTGGTGT AACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTAATCAATCCTGAGTTGATTATTTCACGGCTC TTCACTGAGGTGCTGGATCACAATGAATGTCTAATTATCACAGGTACTGAGCAGTACAGTGAATACACAG GCTATGCTGAGACATATCGTTGGTCCCGGAGCCACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCG CTGCACTGAGATCGTTGCCATCGATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAA ATGAGACGCGAGCTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAGAATCTTTCTG CAGTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGGTTAAAAGCCTTAATACAGAT ATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTCACCTTTGGGGACTCAGAATTGATGAGAGAC ATTTACAGCATGCACATTTTCCTTACTGAAAGGAAACTCACTGTTGGAGATGTGTATAAGCTGTTGCTAC GATACTACAATGAAGAATGCAGAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATA CCATGCTGTCGAGTCCTGTGCAGAGACCGCTGACCATTCAGGGCAAAGGACAGGGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208530 protein sequence
Red=Cloning site Green=Tags(s) MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSAT SLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTE KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQF LTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNK LTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKA EDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLR AGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKY NVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQI ASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRL FTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEK MRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRD IYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003631 |
| ORF Size | 2928 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003631.5 |
| RefSeq Size | 4276 bp |
| RefSeq ORF | 2931 bp |
| Locus ID | 8505 |
| UniProt ID | Q86W56 |
| Domains | PARG |
| MW | 111.1 kDa |
| Gene Summary | Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The ARH and Macrodomain Families of α-ADP-ribose-acceptor Hydrolases Catalyze α-NAD+ Hydrolysis
,Stevens, LA;Kato, J;Kasamatsu, A;Oda, H;Lee, DY;Moss, J;,
ACS Chem. Biol.
,PubMed ID 31599159
[PARG]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208530L1 | Lenti ORF clone of Human poly (ADP-ribose) glycohydrolase (PARG), Myc-DDK-tagged |
CNY 9,552.00 |
|
| RC208530L2 | Lenti ORF clone of Human poly (ADP-ribose) glycohydrolase (PARG), mGFP tagged |
CNY 5,890.00 |
|
| RC208530L3 | Lenti ORF clone of Human poly (ADP-ribose) glycohydrolase (PARG), Myc-DDK-tagged |
CNY 9,552.00 |
|
| RC208530L4 | Lenti ORF clone of Human poly (ADP-ribose) glycohydrolase (PARG), mGFP tagged |
CNY 9,552.00 |
|
| RG208530 | PARG (tGFP-tagged) - Human poly (ADP-ribose) glycohydrolase (PARG) |
CNY 8,752.00 |
|
| SC117822 | PARG (untagged)-Human poly (ADP-ribose) glycohydrolase (PARG) |
CNY 7,152.00 |
|
| SC323845 | PARG (untagged)-Human poly (ADP-ribose) glycohydrolase (PARG) |
CNY 7,152.00 |
