PARG (NM_003631) Human Tagged ORF Clone

CAT#: RG208530

  • TrueORF®

PARG (tGFP-tagged) - Human poly (ADP-ribose) glycohydrolase (PARG)

ORF Plasmid: DDK tGFP



  "NM_003631" in other vectors (7)

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CNY 8,752.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PARG mouse monoclonal antibody, clone OTI6F4 (formerly 6F4)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms PARG99
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208530 representing NM_003631
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATGCGGGCCCCGGCTGTGAACCCTGCACCAAGCGACCCCGCTGGGGCGCCGCTACAACTTCGCCGG
CTGCTTCGGACGCCCGGAGCTTTCCCAGCAGGCAGAGGCGCGTCCTCGACCCCAAGGACGCTCACGTGCA
GTTCAGGGTCCCACCGTCCTCGCCAGCCTGCGTCCCAGGGCGGGCGGGACAGCACAGAGGCAGCGCCACC
TCGCTTGTTTTCAAACAAAAGACTATTACCAGTTGGATGGACACTAAAGGAATCAAGACAGCGGAATCAG
AAAGTTTGGATAGTAAAGAAAACAACAATACAAGAATAGAATCCATGATGAGTTCTGTACAAAAAGATAA
CTTTTACCAACATAATGTAGAAAAATTAGAAAATGTTTCTCAGCTAAGTCTTGATAAGTCACCCACTGAA
AAAAGTACACAGTATTTGAACCAGCATCAGACTGCAGCAATGTGTAAGTGGCAAAATGAAGGGAAACACA
CGGAGCAGCTTTTGGAAAGTGAACCTCAAACAGTAACCCTGGTACCAGAGCAGTTTAGTAATGCTAACAT
TGATCGGTCACCTCAAAATGATGATCACAGTGACACAGATAGTGAAGAGAATAGAGACAATCAACAGTTT
CTCACAACTGTAAAGCTTGCAAATGCAAAGCAGACTACAGAAGATGAACAGGCCAGAGAAGCCAAAAGCC
ACCAGAAGTGCAGCAAGTCTTGCGATCCTGGGGAAGACTGTGCAAGTTGTCAGCAAGATGAGATAGATGT
GGTGCCAGAGAGTCCATTGTCAGATGTTGGCTCTGAGGATGTTGGTACTGGGCCAAAAAATGACAACAAA
TTGACTAGACAAGAAAGTTGCCTAGGAAATTCTCCTCCATTTGAGAAGGAAAGTGAACCCGAGTCACCGA
TGGATGTGGATAATTCTAAAAATAGTTGTCAAGACTCAGAAGCAGATGAGGAGACAAGTCCAGGTTTTGA
TGAACAAGAAGATGGTAGTTCCTCCCAAACAGCAAATAAACCTTCAAGGTTCCAAGCAAGAGACGCTGAC
ATTGAATTTAGGAAACGGTACTCTACTAAGGGCGGTGAAGTTAGATTACATTTCCAATTTGAAGGAGGAG
AGAGTCGCACTGGAATGAATGATTTAAATGCTAAACTACCTGGAAATATTTCTAGCCTGAATGTAGAATG
CAGAAATTCTAAGCAACATGGAAAAAAGGATTCTAAAATCACAGATCATTTCATGAGACTGCCCAAAGCA
GAGGACAGAAGAAAAGAACAGTGGGAAACCAAACATCAAAGAACAGAAAGGAAGATCCCTAAATACGTTC
CACCTCACCTTTCTCCAGATAAGAAGTGGCTTGGAACTCCCATTGAGGAGATGAGAAGAATGCCTCGGTG
TGGGATCCGGCTGCCTCTCTTGAGACCATCTGCCAATCACACAGTAACTATTCGGGTAGATCTTTTGCGA
GCAGGAGAAGTTCCTAAACCTTTTCCAACACATTATAAAGATTTGTGGGATAACAAGCATGTTAAAATGC
CTTGTTCAGAACAAAATTTGTACCCAGTGGAAGATGAGAATGGTGAGCGAACTGCGGGGAGCCGGTGGGA
GCTCATTCAGACTGCACTTCTCAACAAATTTACACGACCCCAAAACTTGAAGGATGCTATTCTGAAATAC
AATGTGGCATATTCTAAGAAATGGGACTTTACAGCTTTGATCGATTTCTGGGATAAGGTACTTGAAGAAG
CAGAAGCTCAACATTTATATCAGTCCATCTTGCCTGATATGGTGAAAATTGCACTCTGTCTGCCAAATAT
TTGCACCCAGCCAATACCACTCCTGAAACAGAAGATGAATCATTCCATCACAATGTCGCAGGAACAGATT
GCCAGTCTTTTAGCTAATGCTTTCTTCTGCACATTTCCACGACGAAATGCTAAGATGAAATCGGAGTATT
CTAGTTACCCAGACATTAACTTCAATCGATTGTTTGAGGGACGTTCATCAAGGAAACCGGAGAAACTTAA
AACGCTCTTCTGCTACTTTAGAAGAGTCACAGAGAAAAAACCTACTGGGTTGGTGACATTTACAAGACAG
AGTCTTGAAGATTTTCCAGAATGGGAAAGATGTGAAAAACCCTTGACACGATTGCATGTCACTTACGAAG
GTACCATAGAAGAAAATGGCCAAGGCATGCTACAGGTGGATTTTGCAAATCGTTTTGTTGGAGGTGGTGT
AACCAGTGCAGGACTTGTGCAAGAAGAAATCCGCTTTTTAATCAATCCTGAGTTGATTATTTCACGGCTC
TTCACTGAGGTGCTGGATCACAATGAATGTCTAATTATCACAGGTACTGAGCAGTACAGTGAATACACAG
GCTATGCTGAGACATATCGTTGGTCCCGGAGCCACGAAGATGGGAGTGAAAGGGACGACTGGCAGCGGCG
CTGCACTGAGATCGTTGCCATCGATGCTCTTCACTTCAGACGCTACCTCGATCAGTTTGTGCCTGAGAAA
ATGAGACGCGAGCTGAACAAGGCTTACTGTGGATTTCTCCGTCCTGGAGTTTCTTCAGAGAATCTTTCTG
CAGTGGCCACAGGAAACTGGGGCTGTGGTGCCTTTGGGGGTGATGCCAGGTTAAAAGCCTTAATACAGAT
ATTGGCAGCTGCTGCAGCTGAGCGAGATGTGGTTTATTTCACCTTTGGGGACTCAGAATTGATGAGAGAC
ATTTACAGCATGCACATTTTCCTTACTGAAAGGAAACTCACTGTTGGAGATGTGTATAAGCTGTTGCTAC
GATACTACAATGAAGAATGCAGAAACTGTTCCACCCCTGGACCAGACATCAAGCTTTATCCATTCATATA
CCATGCTGTCGAGTCCTGTGCAGAGACCGCTGACCATTCAGGGCAAAGGACAGGGACC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208530 representing NM_003631
Red=Cloning site Green=Tags(s)

MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQRRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSAT
SLVFKQKTITSWMDTKGIKTAESESLDSKENNNTRIESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTE
KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTVTLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQF
LTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGEDCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNK
LTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQDSEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD
IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAKLPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKA
EDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLGTPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLR
AGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVEDENGERTAGSRWELIQTALLNKFTRPQNLKDAILKY
NVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILPDMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQI
ASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLFEGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ
SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQVDFANRFVGGGVTSAGLVQEEIRFLINPELIISRL
FTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSHEDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEK
MRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAFGGDARLKALIQILAAAAAERDVVYFTFGDSELMRD
IYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCSTPGPDIKLYPFIYHAVESCAETADHSGQRTGT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003631
ORF Size 2928 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003631.5
RefSeq Size 4276 bp
RefSeq ORF 2931 bp
Locus ID 8505
UniProt ID Q86W56
Domains PARG
Gene Summary Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2015]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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