United States
Germany
Japan
United Kingdom
China
PRR14 (NM_024031) Human Tagged ORF Clone
CAT＃: RC208696
PRR14 (Myc-DDK-tagged)-Human proline rich 14 (PRR14)
ORF Plasmid: tGFP
"NM_024031" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,360.00
CNY 4,560.00
| Cited in 2 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208696 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTGCCCGGGGACTCCAGCCCGCCTGGCCAGCCGCGTCTGTGCCGCCAGCCTCTGACTCGAGCAT TATGGGGAGCCAGGAGCCCGAAACGGCCGAGGCTGCAGCTCCCGGGGGCCCCTTCTCCCCTGGAAAAGGC CTCTCGGCGGGTCCTGGCCGTGGTGCTAGAAGATGTCATGGCTGTTCACATGGTCCCCGTGGTGCCCTCA AAGCAGACCTCCATACCACAGCACCACAGCTACCATCAGGATCCTGTCCACAGGCAGCCGCCTGCCTCGC CACCCCGGCAGGCCGGGTGGTCCTCGCAGGCCAGGCCTCCCGACCCTCTGTGTTTGTGTCGCGAGCCCTT GAGCCGCATCCACCGGACCTCTTCCACCCTGAGGCGGCGATCAAGGACAACCCCTGGCCCAGAGGAGGGC CCTTCACAAAAGGTGGACCGGGCCCCCCAGCCCACCCTGGTGGTGATGCTGGAAGACATCGCCAGTCCTA GACCCCCCGCTGAGGGCTTCATTGATGAGACCCCCAACTTCATCATCCCAGCACAAAGAGCTGAGCCCAT GAGGATAGTTCGCCAGCCAACGCCTCCACCTGGGGACCTAGAACCCCCATTCCAGCCATCTGCTCTGCCT GCAGACCCTCTGGAGAGCCCACCAACAGCCCCAGATCCTGCTCTGGAGCTCCCATCCACCCCACCACCGT CCAGCCTTTTACGCCCCCGCCTCAGTCCCTGGGGCTTGGCCCCGCTCTTCCGTTCCGTCCGCTCCAAGCT GGAGAGCTTTGCTGACATCTTCCTCACGCCCAACAAAACCCCACAGCCCCCACCCCCGTCCCCCCCAATG AAGCTGGAGTTGAAGATCGCCATCTCAGAGGCCGAGCAGTCTGGGGCTGCTGAGGGCACTGCGTCTGTCA GCCCCCGGCCCCCAATCCGCCAGTGGCGAACTCAGGACCACAATACCCCAGCACTTCTCCCTAAGCCCTC TCTGGGCCGAAGCTACTCCTGCCCTGATCTGGGGCCCCCTGGCCCAGGTACCTGCACCTGGCCACCTGCT CCACCCCAACCAAGCCGACCACGGCCGCGGCGGCACACTGTGGGTGGTGGGGAAATGGCCCGAGCCCCGC CACCCCCTCGGCCCTGTCTCCGGAAAGAGGTCTTCCCTCTCGGAGGAGTGGGAGCCTCCCCTTCTCTCAC CACATCTTGCTCGTCCACGGCATCCACTTCCTTCTCCGAACCAGCAGAACCCAGGTTGGGTTCAACCAAA GGGAAGGAGCCAAGAGCCTCAAAGGACCAGGTGCTTTCAGAACCTGAGACCAAGACCATGGGAAAGGTTT CTCGATTCAGAATACGCAGAACACCAGCCCGTCCTCAGCTAAACCTTACACCAATGGGACTGCCTCGACC AATCAGGTTGAACAAGAAGGAGTTCAGCTTGGAAGAAATTTACACCAACAAGAATTACCAATCACCCACA ACCAGGAGGACCTTTGAGACCATCTTTGAGGAACCCCGGGAGCGCAATGGGACTCTGATTTTCACCAGCT CAAGGAAGCTCCGGCGGGCTGTGGAATTTCGGGACAGCAGCCTTCCTCGATCACGAAGACCGTCCCGTGG GGTCCGGGCTGCAGGGGGCAGGACTGTTCCTCCCAATGTGGCCCCCAGCCCTGATGTGGGCCCCCTGCTC CAGCAGCGGCTGGAGGAGCTAGATGCCTTGCTCCTGGAGGAAGAAACAGTAGATCGGGAGCAGCCCCACT GGACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208696 protein sequence
Red=Cloning site Green=Tags(s) MDLPGDSSPPGQPRLCRQPLTRALWGARSPKRPRLQLPGAPSPLEKASRRVLAVVLEDVMAVHMVPVVPS KQTSIPQHHSYHQDPVHRQPPASPPRQAGWSSQARPPDPLCLCREPLSRIHRTSSTLRRRSRTTPGPEEG PSQKVDRAPQPTLVVMLEDIASPRPPAEGFIDETPNFIIPAQRAEPMRIVRQPTPPPGDLEPPFQPSALP ADPLESPPTAPDPALELPSTPPPSSLLRPRLSPWGLAPLFRSVRSKLESFADIFLTPNKTPQPPPPSPPM KLELKIAISEAEQSGAAEGTASVSPRPPIRQWRTQDHNTPALLPKPSLGRSYSCPDLGPPGPGTCTWPPA PPQPSRPRPRRHTVGGGEMARAPPPPRPCLRKEVFPLGGVGASPSLTTSCSSTASTSFSEPAEPRLGSTK GKEPRASKDQVLSEPETKTMGKVSRFRIRRTPARPQLNLTPMGLPRPIRLNKKEFSLEEIYTNKNYQSPT TRRTFETIFEEPRERNGTLIFTSSRKLRRAVEFRDSSLPRSRRPSRGVRAAGGRTVPPNVAPSPDVGPLL QQRLEELDALLLEEETVDREQPHWT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024031 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024031.5 |
| RefSeq Size | 2154 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 78994 |
| UniProt ID | Q9BWN1 |
| MW | 64.3 kDa |
| Gene Summary | The protein encoded by this gene tethers heterochromatin to the nuclear laminar scaffold by binding heterochromatin protein 1 (HP1) and the nuclear lamina. The tether is broken during mitosis and reforms quickly after mitosis, with the encoded protein first binding HP1 and then attaching to the nuclear lamina. This protein also has been shown to promote MyoD activity and skeletal myogenesis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Feb 2016] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina during Interphase and Mitotic Exit
,Poleshko, A;Mansfield, KM;Burlingame, CC;,
Cell Reports, Volume 5, Issue 2, 292-301
[PRR14]
|
|
The Human Protein PRR14 Tethers Heterochromatin to the Nuclear Lamina During Interphase and Mitotic Exit
,null,
Cell reports
,PubMed ID 24209742
[PRR14]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208696L1 | Lenti ORF clone of Human proline rich 14 (PRR14), Myc-DDK-tagged |
CNY 6,760.00 |
|
| RC208696L2 | Lenti ORF clone of Human proline rich 14 (PRR14), mGFP tagged |
CNY 6,460.00 |
|
| RC208696L3 | Lenti ORF clone of Human proline rich 14 (PRR14), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC208696L4 | Lenti ORF clone of Human proline rich 14 (PRR14), mGFP tagged |
CNY 6,460.00 |
|
| RG208696 | PRR14 (tGFP-tagged) - Human proline rich 14 (PRR14) |
CNY 5,960.00 |
|
| SC321888 | PRR14 (untagged)-Human proline rich 14 (PRR14) |
CNY 4,360.00 |
