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DCDC2 (NM_016356) Human Tagged ORF Clone
CAT＃: RC208721
DCDC2 (Myc-DDK-tagged)-Human doublecortin domain containing 2 (DCDC2), transcript variant 1
ORF Plasmid: tGFP
"NM_016356" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DCDC2A; DFNB66; NPHP19; NSC; RU2; RU2S |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208721 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCAGCAGCGCCAGGTCCAGCCACCTGTCTCAGCCCGTCGTGAAGAGCGTGCTTGTGTACCGCA ACGGGGACCCCTTCTACGCGGGGCGCCGCGTCGTCATCCATGAGAAGAAGGTGTCCAGCTTCGAAGTCTT CCTGAAGGAGGTGACCGGCGGCGTTCAGGCACCCTTTGGGGCCGTCAGGAACATCTACACCCCGCGGACT GACCACCGAATCCGGAAGCTAGACCAGATCCAGAGCGGGGGCAATTACGTGGCTGGAGGCCAGGAAGCCT TCAAGAAACTCAATTACTTGGACATAGGAGAAATCAAGAAAAGACCAATGGAAGTTGTTAATACAGAGGT AAAACCAGTAATCCATAGCAGGATCAACGTGTCAGCTCGCTTTAGAAAACCGCTTCAGGAGCCGTGCACT ATCTTCTTGATTGCAAATGGAGACCTCATAAACCCAGCTTCTCGCCTCCTTATCCCCCGAAAAACCTTGA ATCAGTGGGATCATGTACTACAAATGGTCACAGAAAAAATCACTCTGAGGAGCGGGGCTGTTCACAGGCT TTATACTTTAGAAGGAAAACTTGTTGAGAGTGGAGCAGAGTTGGAGAATGGGCAGTTTTATGTGGCTGTT GGCAGAGATAAGTTTAAGAAACTGCCTTACGGTGAGTTACTTTTTGACAAGTCAACGATGAGAAGGCCTT TTGGTCAGAAAGCTTCTTCACTACCTCCTATTGTAGGATCCAGAAAGTCTAAAGGGAGTGGAAATGATCG CCACTCTAAGTCAACAGTTGGATCCAGTGACAACTCATCTCCTCAGCCCCTGAAGAGGAAAGGGAAAAAA GAAGACGTGAATTCAGAAAAACTGACGAAATTGAAACAAAATGTAAAATTAAAGAATTCACAAGAAACCA TTCCAAATAGTGATGAAGGCATTTTCAAAGCTGGAGCAGAGAGGTCTGAAACACGGGGGGCAGCAGAAGT CCAAGAAGATGAAGATACTCAGGTTGAGGTTCCAGTCGATCAGAGGCCAGCAGAAATAGTAGACGAGGAA GAAGATGGAGAGAAGGCAAACAAGGATGCAGAACAGAAAGAAGACTTTTCAGGAATGAATGGTGACCTTG AAGAGGAAGGAGGTAGGGAGGCTACAGATGCCCCTGAGCAAGTCGAGGAGATTCTGGATCACAGTGAGCA GCAGGCACGCCCTGCTCGTGTAAATGGAGGCACCGATGAGGAGAATGGTGAGGAGCTGCAGCAGGTTAAT AATGAGCTTCAACTGGTCCTAGACAAGGAAAGAAAGTCTCAAGGAGCTGGCAGTGGACAAGATGAGGCTG ATGTAGACCCTCAAAGACCACCAAGGCCAGAAGTAAAAATTACCAGTCCAGAAGAAAATGAAAACAACCA ACAAAACAAGGACTATGCTGCCGTGGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208721 protein sequence
Red=Cloning site Green=Tags(s) MSGSSARSSHLSQPVVKSVLVYRNGDPFYAGRRVVIHEKKVSSFEVFLKEVTGGVQAPFGAVRNIYTPRT DHRIRKLDQIQSGGNYVAGGQEAFKKLNYLDIGEIKKRPMEVVNTEVKPVIHSRINVSARFRKPLQEPCT IFLIANGDLINPASRLLIPRKTLNQWDHVLQMVTEKITLRSGAVHRLYTLEGKLVESGAELENGQFYVAV GRDKFKKLPYGELLFDKSTMRRPFGQKASSLPPIVGSRKSKGSGNDRHSKSTVGSSDNSSPQPLKRKGKK EDVNSEKLTKLKQNVKLKNSQETIPNSDEGIFKAGAERSETRGAAEVQEDEDTQVEVPVDQRPAEIVDEE EDGEKANKDAEQKEDFSGMNGDLEEEGGREATDAPEQVEEILDHSEQQARPARVNGGTDEENGEELQQVN NELQLVLDKERKSQGAGSGQDEADVDPQRPPRPEVKITSPEENENNQQNKDYAAVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016356 |
| ORF Size | 1428 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016356.5 |
| RefSeq Size | 4716 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 51473 |
| UniProt ID | Q9UHG0 |
| Domains | DCX |
| MW | 52.9 kDa |
| Gene Summary | This gene encodes a doublecortin domain-containing family member. The doublecortin domain has been demonstrated to bind tubulin and enhance microtubule polymerization. This family member is thought to function in neuronal migration where it may affect the signaling of primary cilia. Mutations in this gene have been associated with reading disability (RD) type 2, also referred to as developmental dyslexia. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208721L1 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC208721L2 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208721L3 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208721L4 | Lenti ORF clone of Human doublecortin domain containing 2 (DCDC2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208721 | DCDC2 (tGFP-tagged) - Human doublecortin domain containing 2 (DCDC2), transcript variant 1 |
CNY 5,256.00 |
|
| SC114336 | DCDC2 (untagged)-Human doublecortin domain containing 2 (DCDC2), transcript variant 1 |
CNY 3,656.00 |
