ZDHHC13 (NM_019028) Human Tagged ORF Clone

CAT#: RC208925

ZDHHC13 (Myc-DDK-tagged)-Human zinc finger, DHHC-type containing 13 (ZDHHC13), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_019028" in other vectors (6)

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CNY 4,632.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Goat Anti-HIP14L / ZDHHC13 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HIP3RP; HIP14L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC208925 representing NM_019028.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGAGGGGCCGGGGCTGGGCTCGCAGTGCAGGAATCACAGCCATGGCCCCCACCCTCCAGGATTTGGT
CGATATGGCATCTGTGCACATGAAAACAAAGAACTTGCCAATGCAAGAGAAGCTCTTCCTCTTATAGAG
GACTCTAGTAACTGTGACATTGTCAAAGCTACTCAATACGGAATTTTTGAACGATGTAAAGAGTTGGTA
GAAGCAGGATATGATGTCAGGCAACCAGATAAAGAAAATGTGTCGCTTCTTCATTGGGCTGCTATTAAC
AACAGACTGGATCTTGTAAGGTTTTATATTTCAAAAGGTGCTGTTGTAGATCAGTTGGGTGGAGATTTA
AATTCAACTCCTCTTCACTGGGCCATCCGACAAGGACATTTACCTATGGTCATATTATTACTCCAGCAT
GGTGCAGACCCCACTCTTATTGATGGAGAGGGATTCAGCAGCATCCACCTGGCAGTATTGTTTCAACAC
ATGCCTATTATAGCATATCTCATCTCAAAGGGACAGAGTGTGAATATGACAGATGTAAATGGGCAGACA
CCTCTCATGTTATCAGCTCACAAAGTAATTGGGCCAGAACCAACTGGATTTCTTTTAAAGTTTAATCCT
TCTCTCAATGTGGTTGATAAAATACACCAAAACACTCCACTTCACTGGGCAGTTGCAGCAGGAAATGTT
AATGCAGTTGATAAGCTTTTGGAAGCTGGTTCTAGCCTGGATATCCAGAATGTTAAGGGAGAAACACCT
CTTGATATGGCTCTACAAAACAAAAATCAGCTCATTATTCATATGCTAAAAACAGAAGCCAAAATGAGA
GCCAACCAAAAGTTCAGACTTTGGAGGTGGCTGCAGAAATGCGAGCTCTTCCTGCTGCTGATGCTTTCT
GTGATTACCATGTGGGCTATTGGATACATATTGGACTTCAATTCAGATTCTTGGCTTTTAAAAGGATGT
CTTCTAGTAACACTGTTTTTTCTGACATCTTTGTTTCCAAGGTTCTTGGTTGGGTATAAGAACCTTGTA
TACTTACCAACAGCCTTTCTGCTAAGTTCTGTTTTTTGGATATTTATGACTTGGTTCATCTTATTTTTT
CCTGATTTAGCAGGAGCCCCTTTCTATTTCAGTTTCATTTTCAGCATAGTAGCCTTTCTATACTTTTTC
TATAAGACTTGGGCAACTGATCCAGGCTTCACTAAGGCTTCTGAAGAAGAAAAGAAAGTGAATATCATC
ACCCTTGCAGAAACTGGCTCTCTGGACTTCAGGACATTTTGTACATCATGTCTTATAAGGAAGCCATTA
AGGTCACTCCACTGCCATGTATGCAACTGCTGTGTGGCTCGATATGATCAACACTGCCTGTGGACTGGA
CGGTGCATAGGTTTTGGCAACCATCACTATTACATATTCTTCTTGTTTTTCCTTTCCATGGTATGTGGC
TGGATTATATATGGATCTTTCATCTATTTGTCCAGTCATTGTGCCACAACATTCAAAGAAGATGGATTA
TGGACTTACCTCAATCAGATTGTGGCCTGTTCCCCTTGGGTTTTATATATCTTGATGCTAGCAACTTTC
CATTTCTCATGGTCAACATTTTTATTATTAAATCAACTCTTTCAGATTGCCTTTCTGGGCCTGACCTCC
CATGAGAGAATCAGCCTGCAGAAGCAGAGCAAGCATATGAAACAGACGTTGTCCCTCAGGAAGACACCA
TACAATCTTGGATTCATGCAGAACCTGGCAGATTTCTTTCAGTGTGGCTGCTTTGGCTTGGTGAAGCCC
TGTGTGGTAGATTGGACATCACAGTATACCATGGTCTTTCACCCAGCCAGGGAGAAGGTTCTTCGCTCA
GTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RC208925
Blue=ORF Red=Cloning site Green=Tag(s)

MEGPGLGSQCRNHSHGPHPPGFGRYGICAHENKELANAREALPLIEDSSNCDIVKATQYGIFERCKELV
EAGYDVRQPDKENVSLLHWAAINNRLDLVRFYISKGAVVDQLGGDLNSTPLHWAIRQGHLPMVILLLQH
GADPTLIDGEGFSSIHLAVLFQHMPIIAYLISKGQSVNMTDVNGQTPLMLSAHKVIGPEPTGFLLKFNP
SLNVVDKIHQNTPLHWAVAAGNVNAVDKLLEAGSSLDIQNVKGETPLDMALQNKNQLIIHMLKTEAKMR
ANQKFRLWRWLQKCELFLLLMLSVITMWAIGYILDFNSDSWLLKGCLLVTLFFLTSLFPRFLVGYKNLV
YLPTAFLLSSVFWIFMTWFILFFPDLAGAPFYFSFIFSIVAFLYFFYKTWATDPGFTKASEEEKKVNII
TLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNCCVARYDQHCLWTGRCIGFGNHHYYIFFLFFLSMVCG
WIIYGSFIYLSSHCATTFKEDGLWTYLNQIVACSPWVLYILMLATFHFSWSTFLLLNQLFQIAFLGLTS
HERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRS
V

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019028
ORF Size 1866 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq Size 2448 bp
RefSeq ORF 1869 bp
Locus ID 54503
UniProt ID Q8IUH4
Domains zf-DHHC, ANK
Protein Families Transmembrane
MW 70.9 kDa
Gene Summary Palmitoyltransferase for HTT and GAD2. May play a role in Mg(2+) transport.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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