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CCDC50 (NM_174908) Human Tagged ORF Clone
CAT＃: RC208965
CCDC50 (Myc-DDK-tagged)-Human coiled-coil domain containing 50 (CCDC50), transcript variant 1
ORF Plasmid: tGFP
"NM_174908" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C3orf6; DFNA44; YMER |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC208965 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAAGTCAGCATCGACCAGTCCAAGCTGCCTGGAGTCAAGGAAGTATGCCGAGATTTTGCTGTCC TGGAGGACCACACCCTGGCTCACAGCCTGCAGGAACAAGAGATTGAGCATCATTTGGCATCGAACGTTCA GCGGAACCGTTTGGTCCAGCATGATCTCCAGGTGGCTAAGCAGCTCCAAGAGGAAGATCTGAAAGCGCAG GCCCAGCTCCAGAAGCGCTACAAAGACCTTGAACAACAAGACTGTGAAATTGCTCAGGAAATTCAGGAGA AGCTGGCTATTGAGGCAGAGAGACGACGCATTCAGGAGAAGAAGGATGAGGACATAGCTCGCCTTTTGCA AGAAAAGGAGTTACAGGAAGAGAAAAAGAGAAAGAAACACTTTCCAGAGTTCCCTGCAACCCGTGCTTAT GCAGATAGTTACTATTATGAAGATGGAGGAATGAAGCCAAGAGTGATGAAAGAAGCTGTATCTACTCCAT CACGAATGGCCCACAGGGATCAGGAATGGTATGATGCTGAAATTGCCAGAAAACTGCAAGAAGAAGAACT TTTGGCTACCCAGGTGGACATGAGAGCCGCTCAAGTAGCTCAAGATGAAGAAATCGCTCGACTTCTAATG GCTGAAGAAAAGAAAGCTTACAAAAAAGCCAAGGAGCGGGAGAAATCATCTTTGGACAAAAGAAAGCAAG ACCCCGAGTGGAAGCCAAAAACAGCTAAAGCAGCAAATTCCAAGTCAAAAGAGAGTGATGAACCTCACCA TTCTAAGAATGAAAGGCCAGCACGGCCACCACCACCTATCATGACAGATGGTGAAGATGCGGATTACACT CATTTTACAAACCAGCAGAGTTCCACACGGCATTTCTCAAAATCAGAGTCCTCTCATAAAGGTTTTCATT ACAAACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC208965 protein sequence
Red=Cloning site Green=Tags(s) MAEVSIDQSKLPGVKEVCRDFAVLEDHTLAHSLQEQEIEHHLASNVQRNRLVQHDLQVAKQLQEEDLKAQ AQLQKRYKDLEQQDCEIAQEIQEKLAIEAERRRIQEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAY ADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLM AEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDEPHHSKNERPARPPPPIMTDGEDADYT HFTNQQSSTRHFSKSESSHKGFHYKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174908 |
| ORF Size | 918 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_174908.4 |
| RefSeq Size | 8421 bp |
| RefSeq ORF | 921 bp |
| Locus ID | 152137 |
| UniProt ID | Q8IVM0 |
| MW | 35.8 kDa |
| Gene Summary | This gene encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208965L1 | Lenti ORF clone of Human coiled-coil domain containing 50 (CCDC50), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC208965L2 | Lenti ORF clone of Human coiled-coil domain containing 50 (CCDC50), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC208965L3 | Lenti ORF clone of Human coiled-coil domain containing 50 (CCDC50), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC208965L4 | Lenti ORF clone of Human coiled-coil domain containing 50 (CCDC50), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG208965 | CCDC50 (tGFP-tagged) - Human coiled-coil domain containing 50 (CCDC50), transcript variant 1 |
CNY 4,000.00 |
|
| SC101288 | CCDC50 (untagged)-Human coiled-coil domain containing 50 (CCDC50), transcript variant 1 |
CNY 2,400.00 |
