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Pinin (PNN) (NM_002687) Human Tagged ORF Clone
CAT＃: RC209069
PNN (Myc-DDK-tagged)-Human pinin, desmosome associated protein (PNN)
ORF Plasmid: tGFP
"NM_002687" in other vectors (6)
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CNY 5,256.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DRS; DRSP; memA; SDK3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209069 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGTCGCCGTAAGAACTTTGCAGGAACAGCTGGAAAAGGCCAAAGAGAGTCTTAAGAACGTGGATG AGAACATTCGCAAGCTCACCGGGCGGGATCCGAATGACGTGAGGCCCATCCAAGCCAGATTGCTGGCCCT TTCTGGTCCTGGTGGAGGTAGAGGACGTGGTAGTTTATTACTGAGGCGTGGATTCTCAGATAGTGGAGGA GGACCCCCAGCCAAACAGAGAGACCTTGAAGGGGCAGTCAGTAGGCTGGGCGGGGAGCGTCGGACCAGAA GAGAATCACGCCAGGAAAGCGACCCGGAGGATGATGATGTTAAAAAGCCAGCATTGCAGTCTTCAGTTGT AGCTACCTCCAAAGAGCGCACACGTAGAGACCTTATCCAGGATCAAAATATGGATGAAAAGGGAAAGCAA AGGAACCGGCGAATATTTGGCTTGTTGATGGGTACCCTTCAAAAATTTAAACAAGAATCCACTGTTGCTA CTGAAAGGCAAAAGCGGCGCCAGGAAATTGAACAAAAACTTGAAGTTCAGGCAGAAGAAGAGAGAAAGCA GGTTGAAAATGAAAGGAGAGAACTGTTTGAAGAGAGGCGTGCTAAACAGACAGAACTGCGGCTTTTGGAA CAGAAAGTTGAGCTTGCGCAGCTGCAAGAAGAATGGAATGAACATAATGCCAAAATAATTAAATATATAA GAACTAAGACAAAGCCCCATTTGTTTTATATTCCTGGAAGAATGTGTCCAGCTACCCAAAAACTAATAGA AGAGTCACAGAGAAAAATGAACGCTTTATTTGAAGGTAGACGCATCGAATTTGCAGAACAAATAAATAAA ATGGAGGCTAGGCCTAGAAGACAATCAATGAAGGAAAAAGAGCATCAGGTGGTGCGTAATGAAGAACAGA AGGCGGAACAAGAAGAGGGTAAGGTGGCTCAGCGAGAGGAAGAGTTGGAGGAGACAGGTAATCAGCACAA TGATGTAGAAATAGAGGAAGCAGGAGAGGAAGAGGAAAAGGAAATAGCGATTGTTCATAGTGATGCAGAG AAAGAACAGGAGGAGGAAGAACAAAAACAGGAAATGGAGGTTAAGATGGAGGAGGAAACTGAGGTAAGGG AAAGTGAGAAGCAGCAGGATAGTCAGCCTGAAGAAGTTATGGATGTGCTAGAGATGGTTGAGAATGTCAA ACATGTAATTGCTGACCAGGAGGTAATGGAAACTAATCGAGTTGAAAGTGTAGAACCTTCAGAAAATGAA GCTAGCAAAGAATTGGAACCAGAAATGGAATTTGAAATTGAGCCAGATAAAGAATGTAAATCCCTTTCTC CTGGGAAAGAGAATGTCAGTGCTTTAGACATGGAAAAGGAGTCTGAGGAAAAAGAAGAAAAAGAATCTGA GCCCCAACCTGAGCCTGTGGCTCAACCTCAGCCTCAGTCTCAGCCCCAGCTTCAGCTTCAATCCCAGTCC CAACCAGTACTCCAGTCCCAGCCTCCCTCTCAGCCTGAGGATTTGTCATTAGCTGTTTTACAGCCAACAC CCCAAGTTACTCAGGAGCAAGGGCATTTACTACCTGAGAGGAAGGATTTTCCTGTAGAGTCTGTAAAACT CACTGAGGTACCAGTAGAGCCAGTCTTGACAGTACATCCAGAGAGCAAGAGCAAAACCAAAACTAGGAGC AGAAGTAGAGGTCGAGCTAGAAATAAAACAAGCAAGAGTAGAAGTCGAAGCAGTAGCAGTAGCAGTTCTA GTAGCAGTTCAACCAGTAGCAGCAGTGGAAGTAGTTCCAGCAGTGGAAGTAGTAGCAGTCGCAGTAGTTC CAGTAGCAGCTCCAGTACAAGTGGCAGCAGCAGCAGAGATAGTAGCAGTAGCACTAGTAGTAGTAGTGAG AGTAGAAGTCGGAGTAGGGGCCGGGGACATAATAGAGATAGAAAGCACAGAAGGAGCGTGGATCGGAAGA GAAGGGATACTTCAGGACTAGAAAGAAGTCACAAATCTTCAAAAGGTGGTAGTAGTAGAGATACAAAAGG ATCAAAGGATAAGAATTCCCGGTCCGACAGAAAGAGGTCTATATCAGAGAGTAGTCGATCAGGCAAAAGA TCTTCAAGAAGTGAAAGAGACCGAAAATCAGACAGGAAAGACAAAAGGCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209069 protein sequence
Red=Cloning site Green=Tags(s) MAVAVRTLQEQLEKAKESLKNVDENIRKLTGRDPNDVRPIQARLLALSGPGGGRGRGSLLLRRGFSDSGG GPPAKQRDLEGAVSRLGGERRTRRESRQESDPEDDDVKKPALQSSVVATSKERTRRDLIQDQNMDEKGKQ RNRRIFGLLMGTLQKFKQESTVATERQKRRQEIEQKLEVQAEEERKQVENERRELFEERRAKQTELRLLE QKVELAQLQEEWNEHNAKIIKYIRTKTKPHLFYIPGRMCPATQKLIEESQRKMNALFEGRRIEFAEQINK MEARPRRQSMKEKEHQVVRNEEQKAEQEEGKVAQREEELEETGNQHNDVEIEEAGEEEEKEIAIVHSDAE KEQEEEEQKQEMEVKMEEETEVRESEKQQDSQPEEVMDVLEMVENVKHVIADQEVMETNRVESVEPSENE ASKELEPEMEFEIEPDKECKSLSPGKENVSALDMEKESEEKEEKESEPQPEPVAQPQPQSQPQLQLQSQS QPVLQSQPPSQPEDLSLAVLQPTPQVTQEQGHLLPERKDFPVESVKLTEVPVEPVLTVHPESKSKTKTRS RSRGRARNKTSKSRSRSSSSSSSSSSSTSSSSGSSSSSGSSSSRSSSSSSSSTSGSSSRDSSSSTSSSSE SRSRSRGRGHNRDRKHRRSVDRKRRDTSGLERSHKSSKGGSSRDTKGSKDKNSRSDRKRSISESSRSGKR SSRSERDRKSDRKDKRR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002687 |
| ORF Size | 2151 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002687.4 |
| RefSeq Size | 3606 bp |
| RefSeq ORF | 2154 bp |
| Locus ID | 5411 |
| UniProt ID | Q9H307 |
| Domains | pinin_SDK_memA, pinin_SDK_N |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| MW | 81.6 kDa |
| Gene Summary | Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene; the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209069L1 | Lenti ORF clone of Human pinin, desmosome associated protein (PNN), Myc-DDK-tagged |
CNY 7,656.00 |
|
| RC209069L2 | Lenti ORF clone of Human pinin, desmosome associated protein (PNN), mGFP tagged |
CNY 7,700.00 |
|
| RC209069L3 | Lenti ORF clone of Human pinin, desmosome associated protein (PNN), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209069L4 | Lenti ORF clone of Human pinin, desmosome associated protein (PNN), mGFP tagged |
CNY 7,700.00 |
|
| RG209069 | PNN (tGFP-tagged) - Human pinin, desmosome associated protein (PNN) |
CNY 6,856.00 |
|
| SC118515 | PNN (untagged)-Human pinin, desmosome associated protein (PNN) |
CNY 8,368.00 |
