ATPSCKMT (NM_199133) Human Tagged ORF Clone
CAT#: RC209093
FAM173B (Myc-DDK-tagged)-Human family with sequence similarity 173, member B (FAM173B)
ORF Plasmid: tGFP
"NM_199133" in other vectors (6)
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CNY 2,400.00
CNY 3,705.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FAM173B; hFAM173B; JS-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209093 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGAGGAGGAGGTATACCCCTAGAAACACTTAAAGAAGAAAGTCAGTCAAGACATGTTCTACCTG CAAGTTTTGAAGTCAACAGTTTGCAGAAAAGCAACTGGGGGTTCTTACTTACTGGGCTTGTGGGTGGCAC CCTGGTGGCTGTGTACGCTGTAGCCACGCCGTTTGTAACGCCAGCCCTTCGAAAAGTCTGTTTGCCATTT GTACCTGCAACTATGAAGCAGATTGAAAATGTTGTGAAAATGTTGCGATGCCGAAGAGGATCCCTTGTGG ACATCGGTAGTGGGGACGGACGCATTGTCATAGCGGCTGCGAAGAAAGGGTTCACAGCAGTTGGTTATGA ATTAAACCCATGGCTAGTTTGGTATTCCAGATACCGCGCTTGGCGAGAAGGTGTGCATGGATCTGCCAAA TTTTATATTTCAGATTTGTGGAAGGTTACTTTTTCGCAGTACTCGAACGTTGTTATTTTCGGTGTGCCTC AGATGATGCTGCAGTTGGAGAAGAAACTTGAACGTGAACTTGAGGATGATGCACGAGTTATTGCTTGCCG GTTCCCTTTCCCACATTGGACTCCAGACCACGTCACGGGGGAGGGGATAGACACAGTGTGGGCATATGAT GCAAGCACTTTTAGAGGCCGTGAAAAGAGGCCCTGTACATCGATGCATTTCCAGCTGCCCATTCAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209093 protein sequence
Red=Cloning site Green=Tags(s) MEGGGGIPLETLKEESQSRHVLPASFEVNSLQKSNWGFLLTGLVGGTLVAVYAVATPFVTPALRKVCLPF VPATMKQIENVVKMLRCRRGSLVDIGSGDGRIVIAAAKKGFTAVGYELNPWLVWYSRYRAWREGVHGSAK FYISDLWKVTFSQYSNVVIFGVPQMMLQLEKKLERELEDDARVIACRFPFPHWTPDHVTGEGIDTVWAYD ASTFRGREKRPCTSMHFQLPIQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_199133 |
ORF Size | 699 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_199133.1, NP_954584.1 |
RefSeq Size | 2672 bp |
RefSeq ORF | 702 bp |
Locus ID | 134145 |
UniProt ID | Q6P4H8 |
Protein Families | Druggable Genome, Transmembrane |
MW | 26.1 kDa |
Gene Summary | Mitochondrial protein-lysine N-methyltransferase that trimethylates ATP synthase subunit C, ATP5MC1 and ATP5MC2. Trimethylation is required for proper incorporation of the C subunit into the ATP synthase complex and mitochondrial respiration (PubMed:29444090, PubMed:30530489). Promotes chronic pain (PubMed:29444090). Involved in persistent inflammatory and neuropathic pain: methyltransferase activity in the mitochondria of sensory neurons promotes chronic pain via a pathway that depends on the production of reactive oxygen species (ROS) and on the engagement of spinal cord microglia (PubMed:29444090).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Identification of FAM173B as a protein methyltransferase promoting chronic pain
,Willemen, HLDM;Kavelaars, A;Prado, J;Maas, M;Versteeg, S;Nellissen, LJJ;Tromp, J;Gonzalez Cano, R;Zhou, W;Jakobsson, ME;Małecki, J;Posthuma, G;Habib, AM;Heijnen, CJ;Falnes, PØ;Eijkelkamp, N;,
PLoS Biol.
,PubMed ID 29444090
[ATPSCKMT]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209093L1 | Lenti ORF clone of Human family with sequence similarity 173, member B (FAM173B), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC209093L2 | Lenti ORF clone of Human family with sequence similarity 173, member B (FAM173B), mGFP tagged |
CNY 5,890.00 |
|
RC209093L3 | Lenti ORF clone of Human family with sequence similarity 173, member B (FAM173B), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209093L4 | Lenti ORF clone of Human family with sequence similarity 173, member B (FAM173B), mGFP tagged |
CNY 5,890.00 |
|
RG209093 | FAM173B (tGFP-tagged) - Human family with sequence similarity 173, member B (FAM173B) |
CNY 4,000.00 |
|
SC322677 | FAM173B (untagged)-Human family with sequence similarity 173, member B (FAM173B) |
CNY 2,400.00 |
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