FKLF / KLF11 (KLF11) (NM_003597) Human Tagged ORF Clone

CAT#: RC209194

KLF11 (Myc-DDK-tagged)-Human Kruppel-like factor 11 (KLF11), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_003597" in other vectors (4)

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CNY 3,816.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


KLF11 mouse monoclonal antibody,clone OTI4H7
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms FKLF; FKLF1; MODY7; TIEG2; Tieg3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACACGCCGGACTTCGCAGGCCCAGACGACGCGCGCGCAGTTGACATCATGGACATATGTGAGTCCA
TCCTGGAGAGGAAGCGGCATGACAGCGAAAGGTCTACTTGCAGCATCTTGGAGCAGACAGACATGGAAGC
TGTCGAGGCTCTTGTTTGTATGAGCTCCTGGGGTCAAAGATCCCAGAAAGGTGACCTGTTGCGGATAAGA
CCCCTCACGCCTGTCTCTGACTCTGGGGATGTCACCACCACTGTGCATATGGATGCAGCCACACCTGAAC
TACCAAAAGACTTCCATTCTTTATCGACTCTGTGCATAACTCCTCCTCAGAGCCCTGATCTCGTGGAGCC
ATCGACAAGGACACCTGTTTCTCCCCAAGTAACAGATTCCAAAGCATGTACAGCCACGGATGTTCTCCAG
TCCTCTGCCGTAGTGGCCAGAGCTCTGAGCGGGGGCGCGGAGAGGGGCTTGCTGGGTTTGGAGCCAGTGC
CCAGCTCTCCCTGCAGGGCCAAGGGGACTAGCGTGATCCGACACACTGGGGAGAGCCCTGCTGCCTGCTT
TCCCACCATCCAGACTCCAGATTGCCGGCTTTCTGACAGCAGAGAAGGAGAAGAGCAGCTTCTGGGACAC
TTTGAAACTTTGCAGGACACACACCTCACGGACAGTTTACTCAGCACTAACTTGGTGTCCTGTCAGCCCT
GCTTGCACAAGTCTGGTGGCCTGCTGCTCACTGACAAAGGCCAGCAGGCAGGGTGGCCTGGTGCAGTTCA
GACTTGCTCACCAAAGAATTATGAAAATGACCTGCCCAGGAAAACCACCCCTCTGATTTCTGTCTCTGTC
CCTGCTCCCCCTGTCCTTTGCCAGATGATCCCTGTGACTGGACAAAGTAGCATGTTACCAGCTTTTTTGA
AGCCCCCTCCCCAGTTGTCTGTGGGGACTGTGAGACCCATCCTAGCTCAGGCTGCTCCAGCGCCTCAACC
TGTGTTCGTGGGACCTGCTGTGCCTCAGGGAGCTGTGATGTTGGTCCTGCCCCAGGGAGCCCTCCCTCCG
CCTGCCCCCTGTGCAGCCAATGTCATGGCTGCCGGGAATACCAAGTTGTTGCCCCTTGCCCCTGCTCCAG
TGTTCATCACCTCTAGCCAAAACTGTGTCCCTCAGGTAGACTTTTCCCGAAGGAGGAACTATGTTTGCAG
CTTCCCAGGTTGCCGGAAGACCTACTTCAAAAGTTCCCACCTTAAGGCCCATCTTCGCACTCACACAGGG
GAGAAGCCTTTCAACTGCAGCTGGGATGGCTGTGATAAAAAGTTTGCTCGTTCGGATGAGCTGTCACGCC
ACCGCAGAACTCACACAGGGGAGAAGAAGTTTGTGTGCCCGGTGTGTGACCGACGTTTCATGCGCAGTGA
CCACCTGACGAAGCATGCCCGGCGCCACATGACGACCAAGAAGATCCCAGGCTGGCAGGCAGAGGTTGGC
AAGCTGAACAGAATCGCCTCTGCAGAGAGCCCGGGGAGCCCACTGGTGAGCATGCCAGCCTCTGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209194 protein sequence
Red=Cloning site Green=Tags(s)

MHTPDFAGPDDARAVDIMDICESILERKRHDSERSTCSILEQTDMEAVEALVCMSSWGQRSQKGDLLRIR
PLTPVSDSGDVTTTVHMDAATPELPKDFHSLSTLCITPPQSPDLVEPSTRTPVSPQVTDSKACTATDVLQ
SSAVVARALSGGAERGLLGLEPVPSSPCRAKGTSVIRHTGESPAACFPTIQTPDCRLSDSREGEEQLLGH
FETLQDTHLTDSLLSTNLVSCQPCLHKSGGLLLTDKGQQAGWPGAVQTCSPKNYENDLPRKTTPLISVSV
PAPPVLCQMIPVTGQSSMLPAFLKPPPQLSVGTVRPILAQAAPAPQPVFVGPAVPQGAVMLVLPQGALPP
PAPCAANVMAAGNTKLLPLAPAPVFITSSQNCVPQVDFSRRRNYVCSFPGCRKTYFKSSHLKAHLRTHTG
EKPFNCSWDGCDKKFARSDELSRHRRTHTGEKKFVCPVCDRRFMRSDHLTKHARRHMTTKKIPGWQAEVG
KLNRIASAESPGSPLVSMPASA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003597
ORF Size 1536 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_003597.4, NP_003588.1
RefSeq Size 4051 bp
RefSeq ORF 1539 bp
Locus ID 8462
UniProt ID O14901
Domains zf-C2H2
Protein Families Transcription Factors
MW 55.1 kDa
Gene Summary The protein encoded by this gene is a zinc finger transcription factor that binds to SP1-like sequences in epsilon- and gamma-globin gene promoters. This binding inhibits cell growth and causes apoptosis. Defects in this gene are a cause of maturity-onset diabetes of the young type 7 (MODY7). Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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