EPM2AIP1 (NM_014805) Human Tagged ORF Clone

CAT#: RC209239

EPM2AIP1 (Myc-DDK-tagged)-Human EPM2A (laforin) interacting protein 1 (EPM2AIP1)

ORF Plasmid: DDK tGFP



  "NM_014805" in other vectors (6)

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CNY 4,520.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


EPM2AIP1 mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209239 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGATGACGCCCAAAAGAAGCAAGATGGAAGTCGACGAGGCTCTAGTTTTCCGGCCCGAGTGGACCC
AGCGTTATTTGGTGGTGGAGCCTCCGGAGGGCGATGGGGCCCTGTGCCTGGTCTGTCGCCGCCTCATCGT
AGCTACCCGCGAACGCGACGTCAGGCGCCACTACGAGGCTGAGCACGAATACTACGAGCGGTATGTGGCG
GACGGCGAGCGCGCGGCCCTGGTGGAGCGTCTGCGTCAGGGCGACTTGCCCGTGGCCTCCTTCACTCCTG
AAGAGAGAGCTGCTCGTGCAGGCCTCGGGCTCTGCCGCCTCTTGGCCTTGAAGGGTCGCGGCTGGGGTGA
GGGGGACTTTGTATACCAGTGCATGGAGGTGTTGCTGAGAGAGGTACTGCCCGAGCATGTAAGCGTCCTG
CAAGGCGTTGACTTATCTCCAGATATCACAAGGCAGAGGATCCTGAGCATTGACAGGAATCTACGCAACC
AGCTTTTTAACCGAGCCAGGGACTTTAAAGCCTATTCTCTTGCCTTGGACGACCAGGCTTTTGTGGCCTA
TGAGAACTACCTCCTGGTCTTTATCCGCGGTGTAGGCCCTGAGTTGGAGGTGCAAGAAGATCTTCTGACC
ATAATCAACCTGACTCATCATTTCAGTGTTGGTGCGCTCATGTCGGCAATCCTAGAGTCCCTGCAGACAG
CAGGGCTTAGCTTGCAGAGAATGGTTGGACTGACCACGACCCATACTTTGAGGATGATTGGTGAGAACTC
AGGACTCGTCTCATACATGAGAGAAAAGGCCGTAAGCCCCAACTGTTGGAATGTCATTCATTATTCAGGA
TTTCTTCACTTGGAACTGTTGAGCTCCTATGATGTAGATGTTAATCAGATCATAAATACCATATCCGAAT
GGATAGTTTTGATTAAGACCAGAGGCGTTAGGCGACCTGAATTTCAGACTTTACTAACGGAATCTGAATC
AGAGCATGGTGAAAGGGTTAATGGACGATGTCTGAACAATTGGCTTAGGAGAGGGAAAACTTTAAAACTA
ATATTCTCTCTAAGAAAAGAAATGGAAGCGTTCTTGGTTTCAGTAGGGGCAACAACAGTCCACTTCTCAG
ACAAACAATGGCTTTGTGACTTTGGCTTCTTGGTGGACATTATGGAACACCTTCGAGAACTCAGTGAAGA
ATTACGAGTTAGTAAAGTCTTTGCTGCTGCTGCCTTTGACCATATTTGTACTTTCGAAGTTAAGCTGAAT
TTATTTCAAAGACATATTGAGGAAAAAAATCTAACAGACTTTCCTGCCCTCAGAGAAGTTGTTGATGAGC
TAAAACAGCAAAATAAGGAAGATGAAAAAATATTTGATCCTGATAGGTATCAAATGGTGATCTGTCGTCT
CCAAAAAGAATTTGAGAGACATTTTAAGGACCTCAGGTTCATTAAAAAGGACTTAGAACTTTTTTCAAAT
CCATTTAACTTTAAACCTGAATATGCACCTATTTCAGTGAGGGTGGAGCTAACAAAACTTCAGGCAAACA
CTAATCTTTGGAATGAATACAGAATCAAAGACTTGGGGCAGTTTTATGCTGGATTGTCTGCTGAATCCTA
CCCAATTATCAAAGGGGTTGCCTGTAAGGTGGCATCCTTGTTTGATAGTAACCAAATCTGTGAAAAGGCT
TTTTCATATTTGACTCGAAACCAACACACTTTGAGTCAGCCATTAACAGATGAGCATCTCCAAGCCCTGT
TTCGGGTTGCCACAACTGAAATGGAGCCCGGTTGGGATGACCTTGTGAGAGAAAGAAATGAATCTAATCC
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209239 protein sequence
Red=Cloning site Green=Tags(s)

MWMTPKRSKMEVDEALVFRPEWTQRYLVVEPPEGDGALCLVCRRLIVATRERDVRRHYEAEHEYYERYVA
DGERAALVERLRQGDLPVASFTPEERAARAGLGLCRLLALKGRGWGEGDFVYQCMEVLLREVLPEHVSVL
QGVDLSPDITRQRILSIDRNLRNQLFNRARDFKAYSLALDDQAFVAYENYLLVFIRGVGPELEVQEDLLT
IINLTHHFSVGALMSAILESLQTAGLSLQRMVGLTTTHTLRMIGENSGLVSYMREKAVSPNCWNVIHYSG
FLHLELLSSYDVDVNQIINTISEWIVLIKTRGVRRPEFQTLLTESESEHGERVNGRCLNNWLRRGKTLKL
IFSLRKEMEAFLVSVGATTVHFSDKQWLCDFGFLVDIMEHLRELSEELRVSKVFAAAAFDHICTFEVKLN
LFQRHIEEKNLTDFPALREVVDELKQQNKEDEKIFDPDRYQMVICRLQKEFERHFKDLRFIKKDLELFSN
PFNFKPEYAPISVRVELTKLQANTNLWNEYRIKDLGQFYAGLSAESYPIIKGVACKVASLFDSNQICEKA
FSYLTRNQHTLSQPLTDEHLQALFRVATTEMEPGWDDLVRERNESNP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014805
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_014805.4
RefSeq Size 7439 bp
RefSeq ORF 1824 bp
Locus ID 9852
UniProt ID Q7L775
MW 70.4 kDa
Gene Summary The EPM2A gene, which encodes laforin, is mutated in an autosomal recessive form of adolescent progressive myoclonus epilepsy. The protein encoded by this gene binds to laforin, but its function is not known. This gene is intronless. [provided by RefSeq, Oct 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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