TUBA3E (NM_207312) Human Tagged ORF Clone

CAT#: RC209279

TUBA3E (Myc-DDK-tagged)-Human tubulin, alpha 3e (TUBA3E)

ORF Plasmid: DDK tGFP



  "NM_207312" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Anti-TUBA3E (Tubulin alpha 3e) mouse monoclonal antibody, clone OTI2A8 (formerly 2A8)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209279 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCGAGTGTATCTCTATCCACGTGGGGCAGGCGGGTGTCCAGATCGGCAATGCCTGCTGGGAACTGT
ACTGCCTTGAACATGGAATTCAGCCCGATGGTCAAATGCCAAGTGATAAAACCATTGGTGGCGGGGACGA
CTCCTTCAACACGTTCTTCAGTGAGACTGGAGCTGGCAAGCACGTGCCCAGAGCAGTGTTTGTGGACCTG
GAGCCCACTGTGGTCGATGAAGTGCGCACAGGGACCTACAGGCAGCTCTTCCACCCAGAGCAGCTGATCA
CCGGGAAGGAAGATGCAGCCAGTAATTACGCCAGGGGCCATTACACCATCGGCAAGGAGATTGTTGACCT
AGTCCTGGACCGGATCCGCAAACTGGCGGATCTGTGCACAGGACTGCAGGGCTTCCTCATCTTCCACAGC
TTTGGGGGCGGCACTGGCTCTGGGTTCGCATCTCTGCTCATGGAGCGGCTCTCAGTGGATTACAGCAAGA
AGTCCAAGCTAGAGTTTGCCATTTACCCAGCCCCCCAGGTCTCCACAGCCGTGGTGGAGCCCTACAACTC
CATCCTAACCACCCACACGACCCTGGAACATTCTGACTGTGCCTTCATGGTCGACAATGAAGCCATCTAT
GACATATGTCGGCGCAACCTGGACATTGAACGTCCCACGTACACCAACCTCAATCGCCTGATTGGGCAGA
TCGTGTCCTCCATCACGGCCTCCCTGCGATTTGATGGGGCCCTGAATGTGGACTTGACGGAATTCCAGAC
CAACCTCGTGCCGTACCCCCGCATCCACTTCCCCCTGGCCACCTACGCCCCAGTCATCTCAGCTGAGAAG
GCCTACCATGAGCAGCTGTCTGTGGCTGAGATCACCAATGCCTGCTTCGAGCCAGCCAATCAGATGGTCA
AGTGTGACCCTCGCCATGGCAAGTACATGGCCTGCTGCATGTTGTACAGGGGGGACGTGGTCCCCAAAGA
CGTCAATGCGGCCATCGCCACCATCAAGACCAAGCGCACTATCCAGTTTGTGGATTGGTGCCCGACTGGA
TTTAAGGTGGGCATTAACTACCAGCCCCCCACAGTGGTCCCCGGGGGAGACCTGGCCAAGGTGCAGCGGG
CCGTGTGCATGCTGAGCAACACCACGGCCATTGCGGAGGCCTGGGCCCGCCTGGTCCATAAGTTCGATCT
CATGTATGCCAAGTGGGCCTTTGTGCACTGGTACGTGGGCGAAGGCATGGAAGAGGGAGAGTTCTCTGAG
GCCCGCGAGGACCTGGCAGCTCTAGAGAAGGATTGTGAAGAGGTGGGCGTGGATTCCGTGGAAGCTGAGG
CTGAAGAAGGCGAAGAATAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209279 protein sequence
Red=Cloning site Green=Tags(s)

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
EPTVVDEVRTGTYRQLFHPEQLITGKEDAASNYARGHYTIGKEIVDLVLDRIRKLADLCTGLQGFLIFHS
FGGGTGSGFASLLMERLSVDYSKKSKLEFAIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
DICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCMLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLVHKFDLMYAKWAFVHWYVGEGMEEGEFSE
AREDLAALEKDCEEVGVDSVEAEAEEGEEY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_207312
ORF Size 1350 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_207312.1, NP_997195.1
RefSeq Size 1554 bp
RefSeq ORF 1353 bp
Locus ID 112714
UniProt ID Q6PEY2
Protein Families Druggable Genome
Protein Pathways Gap junction, Pathogenic Escherichia coli infection
MW 49.9 kDa
Gene Summary Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. This gene encodes an alpha tubulin that highly conserved among species. A missense mutation in this gene has been potentially linked to microlissencephaly and global developmental delay. [provided by RefSeq, Jul 2016]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...