United States
Germany
Japan
United Kingdom
China
POMT1 (NM_001077366) Human Tagged ORF Clone
CAT＃: RC209439
POMT1 (Myc-DDK-tagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3
ORF Plasmid: tGFP
"NM_001077366" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,320.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LGMD2K; LGMDR11; MDDGA1; MDDGB1; MDDGC1; RT |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209439 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGGATTTTTGAAGCGCCCTGTAGTGGTGACGGCTGACATCAACTTGAGCCTTGTGGCCCTGACTG GGATGGGGTTACTGAGCCGGCTGTGGCGACTCACCTACCCGCGGGCTGTGGTTTTTGACGAAGTATATTA TGGGCAGTACATCTCTTTTTACATGAAACAAATCTTCTTCTTGGATGACAGTGGGCCGCCATTTGGCCAC ATGGTGCTGGCCTTGGGAGGTTATTTAGGAGGATTCGATGGCAATTTTTTGTGGAACAGAATTGGAGCAG AATACAGTAGCAACGTGCCTGTGTGGTCCCTGCGCCTGCTGCCAGCACTCGCGGGGGCCTTGTCGGTCCC CATGGCCTACCAGATAGTGTTGGAGCTCCACTTTTCTCATTGTGCCGCCATGGGAGCTGCTCTGTTGATG CTTATCGAGAATGCTCTCATCACTCAGTCAAGGCTAATGCTTTTGGAATCAGTGTTAATATTTTTCAATC TATTGGCCGTGTTGTCCTACCTGAAGTTCTTCAACTGCCAAAAGCACAGCCCTTTTTCTCTGAGCTGGTG GTTCTGGCTAACACTGACAGGGGTCGCTTGTTCCTGTGCAGTGGGCATCAAGTACATGGGTGTGTTCACG TACGTGCTCGTGCTGGGTGTTGCAGCTGTCCATGCCTGGCACCTGCTTGGAGACCAGACTTTGTCCAATG TCTGTGTGTTCTGTCACTTGCTCGCCCGAGCAGTGGCTTTGCTGGTCATCCCGGTCGTCCTGTACTTACT GTTCTTCTACGTCCACTTGATTCTAGTCTTCCGCTCTGGGCCCCACGACCAAATCATGTCCAGTGCCTTC CAGGCCAGCTTAGAGGGAGGACTAGCTCGGATCACCCAGGGTCAGCCACTGGAGGTGGCCTTTGGGTCCC AGGTCACTCTGAGGAACGTCTTTGGGAAACCTGTGCCCTGCTGGCTTCATTCCCACCAGGACACCTACCC CATGATATATGAGAACGGCCGAGGCAGCTCCCACCAGCAACAGGTGACCTGTTACCCCTTCAAAGACGTC AATAACTGGTGGATTGTAAAGGATCCCAGGAGGCACCAGCTGGTGGTGAGCAGCCCTCCGAGACCTGTGA GGCACGGGGACATGGTGCAGCTGGTCCACGGCATGACCACCCGCTCCCTGAACACGCATGATGTTGCAGC CCCCCTGAGCCCCCATTCACAGGAGGTCTCCTGCTACATTGACTATAACATCTCCATGCCCGCCCAGAAC CTCTGGAGACTGGAAATTGTGAACAGAGGATCTGACACAGACGTCTGGAAGACCATCCTCTCAGAGGTCC GCTTTGTGCACGTGAACACTTCCGCTGTCTTAAAGCTGAGCGGGGCTCACCTCCCTGACTGGGGGTATCG GCAACTGGAGATCGTCGGGGAGAAGCTGTCCCGGGGCTACCACGGGAGCACGGTGTGGAACGTGGAGGAG CACCGATACGGCGCGAGCCAGGAGCAGAGGGAGCGGGAACGGGAGCTGCACTCACCTGCGCAGGTGGACG TCAGCAGGAACCTCAGCTTCATGGCGAGATTCTCGGAGCTGCAGTGGAGGATGCTGGCGCTGAGAAGTGA TGACTCGGAACACAAGTACAGCTCCAGCCCACTGGAGTGGGTCACCCTGGACACCAATATTGCCTACTGG CTGCACCCCAGGACCAGCGCTCAGATCCACCTACTTGGAAACATAGTGATCTGGGTTTCGGGCAGCCTCG CTCTGGCCATCTACGCCCTGCTGTCCTTGTGGTACCTGCTCCGACGGCGAAGAAATGTCCATGACCTCCC TCAGGATGCCTGGCTGCGCTGGGTGCTGGCTGGGGCGCTGTGTGCCGGTGGCTGGGCAGTGAACTACCTC CCGTTCTTCCTGATGGAGAAGACACTCTTCCTCTACCACTACCTGCCCGCACTCACCTTCCAAATCCTTC TGCTCCCTGTGGTCCTGCAGCACATCAGCGACCACCTGTGCAGGTCCCAGCTCCAGAGGAGCATCTTCAG CGCCCTGGTGGTGGCCTGGTACTCCTCCGCGTGCCACGTGTCCAACACGCTGCGCCCACTCACCTACGGG GACAAGTCACTCTCGCCACATGAACTCAAGGCCCTTCGCTGGAAAGACAGCTGGGACATCTTGATCCGAA AACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209439 protein sequence
Red=Cloning site Green=Tags(s) MWGFLKRPVVVTADINLSLVALTGMGLLSRLWRLTYPRAVVFDEVYYGQYISFYMKQIFFLDDSGPPFGH MVLALGGYLGGFDGNFLWNRIGAEYSSNVPVWSLRLLPALAGALSVPMAYQIVLELHFSHCAAMGAALLM LIENALITQSRLMLLESVLIFFNLLAVLSYLKFFNCQKHSPFSLSWWFWLTLTGVACSCAVGIKYMGVFT YVLVLGVAAVHAWHLLGDQTLSNVCVFCHLLARAVALLVIPVVLYLLFFYVHLILVFRSGPHDQIMSSAF QASLEGGLARITQGQPLEVAFGSQVTLRNVFGKPVPCWLHSHQDTYPMIYENGRGSSHQQQVTCYPFKDV NNWWIVKDPRRHQLVVSSPPRPVRHGDMVQLVHGMTTRSLNTHDVAAPLSPHSQEVSCYIDYNISMPAQN LWRLEIVNRGSDTDVWKTILSEVRFVHVNTSAVLKLSGAHLPDWGYRQLEIVGEKLSRGYHGSTVWNVEE HRYGASQEQRERERELHSPAQVDVSRNLSFMARFSELQWRMLALRSDDSEHKYSSSPLEWVTLDTNIAYW LHPRTSAQIHLLGNIVIWVSGSLALAIYALLSLWYLLRRRRNVHDLPQDAWLRWVLAGALCAGGWAVNYL PFFLMEKTLFLYHYLPALTFQILLLPVVLQHISDHLCRSQLQRSIFSALVVAWYSSACHVSNTLRPLTYG DKSLSPHELKALRWKDSWDILIRKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077366 |
| ORF Size | 2178 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq Size | 2928 bp |
| RefSeq ORF | 2016 bp |
| Locus ID | 10585 |
| UniProt ID | Q9Y6A1 |
| Protein Families | Transmembrane |
| Protein Pathways | O-Mannosyl glycan biosynthesis |
| MW | 82.6 kDa |
| Gene Summary | The protein encoded by this gene is an O-mannosyltransferase that requires interaction with the product of the POMT2 gene for enzymatic function. The encoded protein is found in the membrane of the endoplasmic reticulum. Defects in this gene are a cause of Walker-Warburg syndrome (WWS) and limb-girdle muscular dystrophy type 2K (LGMD2K). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209439L3 | Lenti ORF clone of Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3, Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC209439L4 | Lenti ORF clone of Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3, mGFP tagged |
CNY 7,700.00 |
|
| RG209439 | POMT1 (tGFP-tagged) - Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3 |
CNY 6,370.00 |
|
| SC315608 | POMT1 (untagged)-Human protein-O-mannosyltransferase 1 (POMT1), transcript variant 3 |
CNY 7,220.00 |
