RNF139 (NM_007218) Human Tagged ORF Clone
CAT#: RC209442
RNF139 (Myc-DDK-tagged)-Human ring finger protein 139 (RNF139)
ORF Plasmid: tGFP
"NM_007218" in other vectors (4)
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CNY 4,944.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HRCA1; RCA1; TRC8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209442 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCCGTGGGGCCCCCGCAGCAGCAGGTGCGGATGGCCCATCAGCAGGTCTGGGCGGCGCTCGAAG TGGCGCTCCGGGTGCCCTGCCTTTACATCATCGACGCCATCTTCAACTCCTACCCGGATTCCAGCCAAAG CCGGTTCTGCATCGTGCTCCAGATCTTCCTCCGGCTCTTTGGTGTATTTGCATCCAGTATTGTTCTGATC TTGTCACAACGATCACTTTTCAAGTTTTACACGTACAGCTCAGCCTTTCTGTTAGCTGCAACTTCAGTGT TGGTGAATTATTATGCTTCTTTGCACATTGACTTCTATGGTGCCTACAACACGTCAGCTTTTGGAATTGA GCTGCTTCCTCGAAAAGGTCCCTCGCTGTGGATGGCACTTATCGTTCTACAGCTAACATTTGGAATTGGA TACGTTACACTACTCCAGATTCATTCCATCTATTCACAATTAATTATTTTGGATCTCTTGGTTCCTGTAA TAGGCTTAATCACAGAGCTACCATTACACATCAGAGAGACTTTACTGTTTACTTCTTCCTTGATTCTCAC ATTAAATACAGTGTTTGTCCTGGCAGTGAAACTGAAGTGGTTTTATTATTCCACACGATATGTTTATCTT TTGGTGAGGCACATGTATCGAATTTACGGATTACAGTTATTGATGGAGGACACATGGAAGAGGATTCGTT TCCCAGACATACTACGAGTCTTTTGGCTAACAAGAGTTACAGCTCAGGCTACAGTGTTAATGTACATCTT AAGGATGGCAAATGAAACTGATTCCTTCTTTATTTCTTGGGATGATTTTTGGGACCTCATTTGCAATCTT ATAATTAGTGGGTGCGATTCTACACTAACTGTACTGGGCATGAGTGCTGTAATTTCCTCAGTAGCCCATT ATTTGGGGCTTGGAATATTGGCCTTTATTGGATCAACTGAGGAAGATGACAGGCGTCTTGGCTTTGTTGC ACCTGTTTTATTTTTTATTTTGGCTCTTCAGACTGGGTTAAGTGGGCTAAGACCAGAAGAGAGACTTATT CGCTTAAGTAGAAACATGTGCCTTTTATTAACTGCAGTCCTGCATTTTATCCATGGAATGACAGACCCTG TATTAATGTCTCTCAGTGCCTCTCATGTGTCATCTTTTCGTAGACATTTTCCTGTGCTGTTTGTCTCTGC TTGCCTGTTTATTCTTCCTGTCTTACTCAGTTATGTTCTTTGGCATCACTATGCACTAAATACATGGTTG TTTGCAGTTACAGCATTTTGTGTGGAACTGTGCTTAAAAGTAATTGTTTCTCTCACTGTTTATACGTTAT TCATGATTGATGGCTACTATAATGTCCTCTGGGAAAAGCTTGACGATTATGTCTACTACGTTCGTTCAAC AGGCAGTATTATTGAATTTATATTTGGAGTTGTAATGTTTGGAAATGGGGCTTACACTATGATGTTTGAG TCGGGAAGTAAAATTCGGGCTTTTATGATGTGCCTACATGCATATTTTAACATCTACTTACAAGCCAAAA ATGGCTGGAAGACATTTATGAATCGTAGGACTGCTGTGAAGAAAATTAATTCACTTCCTGAAATAAAAGG GAGCCGCTTACAAGAAATAAATGATGTATGTGCAATCTGCTATCATGAGTTTACAACATCTGCTCGTATT ACACCGTGTAATCATTATTTCCATGCACTTTGCCTTCGGAAATGGCTGTACATTCAAGATACTTGTCCAA TGTGCCATCAGAAAGTATACATCGAAGATGATATCAAGGATAATTCAAATGTATCTAACAACAATGGATT TATTCCACCCAATGAAACTCCAGAGGAAGCTGTAAGAGAAGCTGCTGCTGAATCTGACAGGGAATTGAAC GAAGATGACAGTACAGATTGTGATGATGATGTTCAAAGAGAAAGAAATGGAGTGATTCAGCACACAGGCG CAGCAGCTGAAGAATTTAATGATGATACTGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209442 protein sequence
Red=Cloning site Green=Tags(s) MAAVGPPQQQVRMAHQQVWAALEVALRVPCLYIIDAIFNSYPDSSQSRFCIVLQIFLRLFGVFASSIVLI LSQRSLFKFYTYSSAFLLAATSVLVNYYASLHIDFYGAYNTSAFGIELLPRKGPSLWMALIVLQLTFGIG YVTLLQIHSIYSQLIILDLLVPVIGLITELPLHIRETLLFTSSLILTLNTVFVLAVKLKWFYYSTRYVYL LVRHMYRIYGLQLLMEDTWKRIRFPDILRVFWLTRVTAQATVLMYILRMANETDSFFISWDDFWDLICNL IISGCDSTLTVLGMSAVISSVAHYLGLGILAFIGSTEEDDRRLGFVAPVLFFILALQTGLSGLRPEERLI RLSRNMCLLLTAVLHFIHGMTDPVLMSLSASHVSSFRRHFPVLFVSACLFILPVLLSYVLWHHYALNTWL FAVTAFCVELCLKVIVSLTVYTLFMIDGYYNVLWEKLDDYVYYVRSTGSIIEFIFGVVMFGNGAYTMMFE SGSKIRAFMMCLHAYFNIYLQAKNGWKTFMNRRTAVKKINSLPEIKGSRLQEINDVCAICYHEFTTSARI TPCNHYFHALCLRKWLYIQDTCPMCHQKVYIEDDIKDNSNVSNNNGFIPPNETPEEAVREAAAESDRELN EDDSTDCDDDVQRERNGVIQHTGAAAEEFNDDTD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007218 |
ORF Size | 1992 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007218.4 |
RefSeq Size | 3312 bp |
RefSeq ORF | 1995 bp |
Locus ID | 11236 |
UniProt ID | Q8WU17 |
Domains | RING |
Protein Families | Druggable Genome, Transmembrane |
MW | 76 kDa |
Gene Summary | The protein encoded by this gene is a multi-membrane spanning protein containing a RING-H2 finger. This protein is located in the endoplasmic reticulum, and has been shown to possess ubiquitin ligase activity. This gene was found to be interrupted by a t(3:8) translocation in a family with hereditary renal and non-medulary thyroid cancer. Studies of the Drosophila counterpart suggested that this protein may interact with tumor suppressor protein VHL, as well as with COPS5/JAB1, a protein responsible for the degradation of tumor suppressor CDKN1B/P27KIP. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209442L3 | Lenti ORF clone of Human ring finger protein 139 (RNF139), Myc-DDK-tagged |
CNY 6,460.00 |
|
RC209442L4 | Lenti ORF clone of Human ring finger protein 139 (RNF139), mGFP tagged |
CNY 6,460.00 |
|
RG209442 | RNF139 (tGFP-tagged) - Human ring finger protein 139 (RNF139) |
CNY 6,544.00 |
|
SC115641 | RNF139 (untagged)-Human ring finger protein 139 (RNF139) |
CNY 4,952.00 |