USP3 (NM_006537) Human Tagged ORF Clone
CAT#: RC209550
- TrueORF®
USP3 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 3 (USP3)
ORF Plasmid: tGFP
"NM_006537" in other vectors (4)
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CNY 3,872.00
CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SIH003; UBP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC209550 representing NM_006537
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTGTCCACACCTGAGCTCCAGCGTCTGCATTGCTCCGGACTCAGCCAAGTTCCCCAACGGCTCCC CGTCGTCCTGGTGCTGCAGCGTGTGCCGGTCCAACAAAAGCCCTTGGGTCTGTTTGACTTGTTCAAGTGT CCACTGTGGAAGGTATGTGAATGGCCATGCAAAAAAACATTATGAAGATGCACAAGTACCTTTAACCAAC CATAAGAAATCAGAAAAGCAAGATAAAGTTCAGCACACAGTATGTATGGATTGCAGTAGCTACAGTACAT ACTGTTATCGCTGTGATGATTTTGTGGTTAATGACACCAAGCTGGGACTGGTACAGAAAGTCAGAGAACA CTTACAGAACTTGGAAAACTCAGCTTTCACAGCTGACAGGCATAAGAAAAGAAAACTTTTGGAAAACTCA ACACTAAACAGCAAGTTATTAAAAGTAAATGGAAGCACCACTGCCATTTGTGCCACAGGCCTTCGGAATT TGGGGAACACATGTTTCATGAATGCCATCCTTCAGTCACTCAGTAACATTGAGCAGTTTTGCTGTTATTT CAAAGAACTGCCCGCCGTGGAGTTAAGGAATGGGAAAACAGCAGGAAGGCGGACATACCACACCAGGAGC CAAGGGGATAACAATGTGTCTTTGGTAGAAGAGTTTAGAAAGACACTCTGTGCTTTATGGCAAGGCAGCC AGACTGCATTTAGCCCAGAGTCCTTATTTTATGTTGTTTGGAAGATTATGCCAAACTTTAGGGGCTATCA ACAGCAGGACGCCCATGAATTCATGCGCTACCTTTTGGACCACCTACACTTGGAACTTCAGGGCGGTTTC AACGGTGTTTCCCGCTCAGCAATTCTGCAGGAGAATTCTACTCTGTCTGCAAGTAACAAGTGTTGCATAA ATGGAGCATCTACTGTTGTCACGGCTATATTCGGAGGCATTCTCCAAAATGAGGTTAACTGCCTCATATG TGGGACAGAATCTAGAAAGTTTGATCCATTCCTAGACCTTTCATTAGATATTCCAAGTCAGTTCAGAAGT AAGCGCTCTAAGAATCAAGAAAATGGACCAGTTTGTTCGTTACGAGATTGTCTTCGCAGTTTTACCGACT TAGAAGAACTTGATGAGACAGAGTTATATATGTGCCATAAATGCAAAAAGAAACAAAAGTCCACAAAAAA GTTTTGGATTCAAAAACTACCCAAGGTGCTATGCTTACATTTGAAAAGATTTCATTGGACAGCATATTTA AGAAATAAAGTTGATACATACGTAGAATTTCCACTGAGAGGCCTAGACATGAAATGCTACTTACTAGAGC CTGAGAACAGTGGCCCGGAGAGCTGCCTGTATGACCTCGCCGCTGTGGTGGTGCACCATGGTTCCGGGGT TGGTTCTGGACATTACACAGCATACGCAACTCACGAAGGCCGCTGGTTCCACTTCAATGACAGTACTGTA ACACTGACTGACGAGGAGACTGTGGTGAAGGCGAAGGCCTACATCCTTTTCTACGTGGAACACCAGGCCA AAGCTGGATCGGATAAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209550 representing NM_006537
Red=Cloning site Green=Tags(s) MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTN HKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENS TLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRS QGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGF NGVSRSAILQENSTLSASNKCCINGASTVVTAIFGGILQNEVNCLICGTESRKFDPFLDLSLDIPSQFRS KRSKNQENGPVCSLRDCLRSFTDLEELDETELYMCHKCKKKQKSTKKFWIQKLPKVLCLHLKRFHWTAYL RNKVDTYVEFPLRGLDMKCYLLEPENSGPESCLYDLAAVVVHHGSGVGSGHYTAYATHEGRWFHFNDSTV TLTDEETVVKAKAYILFYVEHQAKAGSDKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_006537 |
ORF Size | 1560 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006537.4 |
RefSeq Size | 5606 bp |
RefSeq ORF | 1563 bp |
Locus ID | 9960 |
UniProt ID | Q9Y6I4 |
Domains | UCH, zf-UBP |
Protein Families | Druggable Genome, Protease |
MW | 59.3 kDa |
Gene Summary | Hydrolase that deubiquitinates monoubiquitinated target proteins such as histone H2A and H2B. Required for proper progression through S phase and subsequent mitotic entry. May regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. Associates with the chromatin.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209550L3 | Lenti-ORF clone of USP3 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 3 (USP3) |
CNY 5,890.00 |
|
RC209550L4 | Lenti-ORF clone of USP3 (mGFP-tagged)-Human ubiquitin specific peptidase 3 (USP3) |
CNY 5,890.00 |
|
RG209550 | USP3 (tGFP-tagged) - Human ubiquitin specific peptidase 3 (USP3) |
CNY 5,472.00 |
|
SC116024 | USP3 (untagged)-Human ubiquitin specific peptidase 3 (USP3) |
CNY 3,880.00 |