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GM130 (GOLGA2) (NM_004486) Human Tagged ORF Clone
CAT＃: RC209641
- TrueORF®
GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2)
ORF Plasmid: tGFP
"NM_004486" in other vectors (7)
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CNY 10,464.00
| Cited in 2 publications. |
Product images
CNY 300.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GM130 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC209641 representing NM_004486
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGCCCCAACCCCGCCTCCCTCCCCGCCCCGCGATGTCGGAAGAAACCCGACAGAGCAAATTGGCCG CAGCGAAGAAAAAGTTGAGAGAATATCAGCAGAGGAATAGCCCTGGTGTTCCTACAGGAGCGAAAAAGAA GAAGAAAATAAAAAATGGCAGTAACCCTGAGACAACCACTTCTGGTGGTTGCCACTCACCTGAGGATACA CCCAAGGACAATGCTGCTACTCTACAACCATCTGATGACACCGTGTTACCTGGCGGTGTCCCTTCCCCTG GTGCCAGTCTCACTAGCATGGCGGCATCTCAGAATCATGATGCTGACAATGTCCCTAATCTCATGGATGA AACCAAGACTTTCTCATCAACCGAGAGCCTGCGACAACTCTCCCAACAGCTCAATGGTCTTGTTTGTGAG TCTGCGACATGTGTCAATGGGGAGGGCCCTGCATCGTCTGCTAACCTGAAGGATCTGGAGAGCCGGTACC AACAGCTAGCGGTAGCCCTGGACTCCAGCTATGTAACAAACAAACAACTCAATATCACGATAGAGAAATT GAAACAACAGAACCAAGAAATTACGGATCAGTTGGAAGAAGAAAAGAAAGAATGCCACCAAAAGCAGGGA GCCCTAAGGGAGCAGTTACAGGTTCACATTCAGACCATAGGGATCCTCGTATCAGAGAAAGCTGAGTTAC AGACAGCCCTGGCTCACACTCAGCATGCTGCCAGGCAGAAAGAAGGAGAGTCTGAAGATCTGGCCAGCCG CCTGCAGTATTCCCGGCGGCGTGTGGGAGAGTTGGAGCGGGCTCTCTCTGCTGTCTCCACGCAGCAGAAG AAGGCAGACAGGTACAACAAGGAGTTAACCAAAGAGAGAGACGCCCTCAGGCTGGAGTTATACAAGAACA CCCAAAGCAATGAGGACCTGAAGCAAGAGAAATCAGAATTGGAAGAGAAGCTTCGGGTCCTAGTGACTGA GAAGGCTGGCATGCAGCTTAACTTGGAAGAATTGCAAAAGAAGTTAGAGATGACGGAACTCCTGCTTCAA CAGTTTTCAAGCCGGTGTGAAGCCCCTGATGCTAACCAGCAGTTACAGCAGGCCATGGAGGAGCGGGCAC AGCTGGAAGCACACCTGGGGCAGGTAATGGAGTCGGTTAGACAACTACAAATGGAGAGAGATAAATATGC GGAGAATCTCAAAGGAGAGAGCGCCATGTGGCGGCAGAGGATGCAGCAGATGTCAGAGCAGGTGCACACA TTGAGAGAGGAGAAGGAATGTAGCATGAGTCGGGTACAGGAGCTGGAGACGAGCTTGGCTGAACTGAGGA ACCAGATGGCTGAACCCCCGCCCCCAGAGCCCCCAGCAGGGCCCTCCGAGGTGGAGCAGCAGCTACAAGC GGAGGCTGAGCACCTGCGGAAGGAGCTGGAGGGTCTGGCAGGACAGCTTCAAGCCCAGGTGCAAGACAAT GAGGGCTTGAGTCGCCTGAACCGGGAGCAGGAGGAGAGGCTGCTGGAGCTGGAGCGGGCGGCCGAGCTCT GGGGGGAGCAGGCGGAGGCGCGCAGGCAAATCCTGGAGACCATGCAGAACGACCGCACTACCATCAGCCG CGCACTCTCCCAGAACCGGGAGCTCAAGGAGCAGCTGGCTGAGCTGCAGAGCGGATTTGTAAAGCTGACT AATGAGAACATGGAGATCACCAGCGCACTGCAGTCGGAGCAGCACGTCAAGAGGGAGCTGGGAAAGAAGC TGGGCGAGCTGCAGGAGAAGCTGAGCGAGCTGAAGGAAACGGTGGAGCTGAAGAGCCAAGAGGCTCAAAG TCTGCAGCAGCAGCGAGACCAGTACCTGGGACACCTGCAGCAGTATGTGGCCGCCTATCAGCAGCTGACC TCTGAGAAGGAGGTGCTGCATAATCAGCTACTGCTGCAGACCCAGCTCGTGGACCAGCTGCAGCAGCAGG AAGCTCAGGGCAAAGCGGTGGCCGAGATGGCCCGCCAAGAGTTGCAGGAAACCCAGGAGCGCCTGGAAGC TGCCACCCAGCAGAATCAGCAGCTACGGGCCCAGTTGAGCCTCATGGCTCACCCTGGGGAAGGAGATGGA CTGGACCGGGAGGAGGAGGAGGATGAGGAGGAGGAGGAGGAGGAGGCGGTGGCAGTACCTCAGCCCATGC CAAGCATCCCGGAGGACCTGGAGAGCCGGGAAGCCATGGTGGCATTTTTCAACTCAGCTGTAGCCAGTGC CGAGGAGGAGCAGGCAAGGCTACGTGGGCAGCTGAAGGAGCAAAGGGTGCGCTGCCGGCGCCTGGCTCAC CTGCTGGCCTCGGCCCAGAAGGAGCCTGAGGCAGCAGCCCCAGCCCCAGGGACCGGGGGTGATTCTGTGT GTGGGGAGACCCACCGGGCCCTGCAGGGGGCCATGGAGAAGCTGCAGAGCCGCTTTATGGAGCTCATGCA GGAGAAGGCAGACCTGAAGGAGAGGGTAGAGGAACTGGAACATCGCTGCATCCAGCTTTCTGGAGAGACA GACACCATTGGAGAGTACATTGCACTGTACCAGAGCCAGAGGGCAGTGCTGAAGGAGCGGCACCGGGAGA AGGAGGAGTACATCAGCAGGCTGGCCCAAGACAAGGAGGAGATGAAGGTGAAGCTGCTGGAGCTGCAGGA GCTGGTCTTACGGCTTGTGGGCGACCGCAACGAGTGGCATGGCAGATTCCTGGCAGCTGCCCAGAACCCT GCTGATGAGCCCACTTCAGGGGCCCCAGCCCCCCAGGAACTTGGGGCTGCCAACCAGCAGGGTGATCTTT GCGAGGTGAGCCTCGCCGGCAGTGTGGAGCCTGCCCAAGGAGAGGCCAGGGAGGGTTCTCCCCGTGACAA CCCCACTGCACAGCAGATCATGCAGCTGCTTCGTGAGATGCAGAACCCCCGGGAGCGCCCAGGCTTGGGC AGCAACCCCTGCATTCCTTTTTTTTACCGGGCTGACGAGAATGATGAGGTGAAGATCACTGTCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC209641 representing NM_004486
Red=Cloning site Green=Tags(s) MWPQPRLPPRPAMSEETRQSKLAAAKKKLREYQQRNSPGVPTGAKKKKKIKNGSNPETTTSGGCHSPEDT PKDNAATLQPSDDTVLPGGVPSPGASLTSMAASQNHDADNVPNLMDETKTFSSTESLRQLSQQLNGLVCE SATCVNGEGPASSANLKDLESRYQQLAVALDSSYVTNKQLNITIEKLKQQNQEITDQLEEEKKECHQKQG ALREQLQVHIQTIGILVSEKAELQTALAHTQHAARQKEGESEDLASRLQYSRRRVGELERALSAVSTQQK KADRYNKELTKERDALRLELYKNTQSNEDLKQEKSELEEKLRVLVTEKAGMQLNLEELQKKLEMTELLLQ QFSSRCEAPDANQQLQQAMEERAQLEAHLGQVMESVRQLQMERDKYAENLKGESAMWRQRMQQMSEQVHT LREEKECSMSRVQELETSLAELRNQMAEPPPPEPPAGPSEVEQQLQAEAEHLRKELEGLAGQLQAQVQDN EGLSRLNREQEERLLELERAAELWGEQAEARRQILETMQNDRTTISRALSQNRELKEQLAELQSGFVKLT NENMEITSALQSEQHVKRELGKKLGELQEKLSELKETVELKSQEAQSLQQQRDQYLGHLQQYVAAYQQLT SEKEVLHNQLLLQTQLVDQLQQQEAQGKAVAEMARQELQETQERLEAATQQNQQLRAQLSLMAHPGEGDG LDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAH LLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGET DTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNP ADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLG SNPCIPFFYRADENDEVKITVI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004486 |
| ORF Size | 3006 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004486.4, NP_004477.3 |
| RefSeq Size | 4304 bp |
| RefSeq ORF | 3009 bp |
| Locus ID | 2801 |
| UniProt ID | Q08379 |
| Domains | M |
| MW | 113.5 kDa |
| Gene Summary | The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The golgin family exhibits a propensity to form condensates in living cells
,Ziltener, P;Rebane, AA;Graham, M;Ernst, AM;Rothman, JE;,
FEBS Lett.
,PubMed ID 32668013
[GOLGA2]
|
|
Liquid-liquid phase separation of the Golgi matrix protein GM130
,Rebane, AA;Ziltener, P;LaMonica, LC;Bauer, AH;Zheng, H;López-Montero, I;Pincet, F;Rothman, JE;Ernst, AM;,
FEBS Lett.
,PubMed ID 31833055
[GOLGA2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209641L1 | Lenti-ORF clone of GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2) |
CNY 12,864.00 |
|
| RC209641L2 | Lenti-ORF clone of GOLGA2 (mGFP-tagged)-Human golgin A2 (GOLGA2) |
CNY 12,864.00 |
|
| RC209641L3 | Lenti-ORF clone of GOLGA2 (Myc-DDK-tagged)-Human golgin A2 (GOLGA2) |
CNY 7,600.00 |
|
| RC209641L4 | Lenti-ORF clone of GOLGA2 (mGFP-tagged)-Human golgin A2 (GOLGA2) |
CNY 7,600.00 |
|
| RG209641 | GOLGA2 (tGFP-tagged) - Human golgin A2 (GOLGA2) |
CNY 12,064.00 |
|
| SC310019 | GOLGA2 (untagged)-Human golgin A2 (GOLGA2) |
CNY 10,472.00 |
|
| SC321016 | GOLGA2 (untagged)-Human golgin A2 (GOLGA2) |
CNY 10,472.00 |
