SNRK (NM_017719) Human Tagged ORF Clone

CAT#: RC209726

SNRK (Myc-DDK-tagged)-Human SNF related kinase (SNRK), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_017719" in other vectors (7)

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CNY 5,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit polyclonal antibody to SNRK (SNF related kinase)
    • 100 ul

CNY 5,863.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms HSNFRK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGGTTATATGATCTGGATAAAACCTTGGGTC
GAGGCCATTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGTGAAAAGGTGGCAGTAAAAGTTAT
TGACAAGACAAAACTGGACACTCTAGCTACTGGTCATCTTTTCCAGGAAGTGAGATGCATGAAACTAGTG
CAGCATCCTAACATCGTCCGCCTTTATGAAGTTATTGACACCCAGACCAAACTATATCTTATTCTAGAAC
TTGGGGATGGAGGAGATATGTTTGATTATATAATGAAACATGAGGAGGGTCTTAATGAAGACTTGGCCAA
GAAGTATTTTGCTCAGATAGTTCATGCTATATCTTATTGCCATAAACTCCATGTGGTTCACAGAGACTTA
AAACCAGAGAATGTAGTCTTCTTTGAAAAACAAGGTCTTGTAAAGTTGACAGACTTTGGGTTCAGCAACA
AATTTCAACCAGGGAAGAAGCTCACTACAAGCTGTGGATCTCTTGCATATTCCGCTCCAGAAATTCTGCT
TGGTGATGAGTATGATGCACCTGCAGTAGATATTTGGAGTCTGGGAGTGATCCTTTTCATGTTGGTGTGT
GGGCAGCCGCCCTTTCAAGAAGCCAATGACAGTGAAACACTGACAATGATCATGGATTGCAAATATACAG
TACCATCCCATGTGTCTAAAGAGTGTAAAGACCTAATCACACGGATGCTACAGAGAGATCCCAAGAGAAG
GGCTTATTTAGAAGAGATTGAAAATCATCCTTGGCTTCAGGGAGTGGACCCTTCACCAGCTACAAAGTAT
AACATTCCCCTTGTGTCATACAAAAATCTCTCGGAAGAGGAGCACAACAGCATCATTCAGCGCATGGTGC
TTGGGGACATAGCGGATCGAGACGCCATTGTAGAAGCCCTGGAAACCAACAGGTATAACCATATCACAGC
CACATACTTCCTGCTGGCTGAAAGGATCCTGAGAGAAAAGCAAGAGAAAGAAATACAGACCAGATCTGCA
AGCCCGAGCAATATCAAGGCCCAGTTTAGGCAGTCATGGCCAACCAAAATTGATGTACCCCAGGACCTTG
AGGATGACCTCACGGCCACTCCTTTGTCCCACGCGACTGTCCCTCAGTCTCCTGCTCGGGCTGCTGACAG
TGTCCTCAATGGCCACAGGAGCAAAGGCCTGTGTGACTCAGCTAAGAAAGATGACCTCCCTGAGTTGGCT
GGACCAGCACTCTCTACGGTGCCACCCGCAAGCTTAAAACCCACAGCCAGTGGGCGGAAGTGTCTGTTCA
GGGTGGAAGAAGATGAAGAGGAAGATGAGGAGGACAAGAAACCCATGTCCCTCTCAACACAAGTGGTTTT
GCGCCGGAAGCCATCTGTAACCAACCGCCTGACATCCAGGAAGAGTGCGCCCGTCGTCAACCAGATCTTT
GAGGAAGGGGAATCTGACGATGAGTTTGACATGGATGAGAATCTGCCTCCCAAGTTGAGCAGGTTAAAGA
TGAATATAGCTTCTCCAGGTACAGTTCACAAACGCTACCACCGGAGGAAAAGTCAGGGCCGGGGCTCCAG
CTGCAGTAGTTCGGAGACCAGTGATGATGATTCTGAAAGCCGGCGGCGGCTCGATAAAGATAGCGGGTTC
ACCTACTCCTGGCACCGACGGGATAGCAGCGAGGGGCCCCCTGGCAGTGAGGGGGATGGCGGGGGCCAGA
GCAAGCCAAGCAATGCCAGTGGAGGGGTGGACAAGGCCAGCCCCAGTGAGAACAATGCTGGTGGGGGCAG
TCCCTCCAGCGGCTCGGGTGGCAACCCCACCAATACATCGGGTACCACACGCCGCTGTGCCGGCCCAAGC
AACTCCATGCAGCTGGCCTCTCGCAGTGCTGGGGAGCTCGTTGAGAGCCTCAAACTCATGAGCCTCTGCC
TCGGCTCCCAGCTTCATGGGAGCACCAAGTACATTATTGATCCACAGAATGGCTTGTCATTTTCCAGTGT
GAAAGTCCAAGAGAAATCTACGTGGAAAATGTGCATTAGCTCCACAGGGAATGCAGGGCAGGTCCCTGCA
GTGGGCGGCATAAAGTTTTTCTCTGACCACATGGCAGATACCACCACTGAATTGGAACGGATAAGGAGCA
AGAACCTAAAAAATAACGTGCTGCAGCTACCTCTGTGCGAAAAGACCATCTCTGTGAACATCCAGCGGAA
CCCTAAGGAGGGGCTGCTGTGCGCATCCAGCCCAGCCAGCTGTTGCCATGTCATC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209726 protein sequence
Red=Cloning site Green=Tags(s)

MAGFKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTLATGHLFQEVRCMKLV
QHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYIMKHEEGLNEDLAKKYFAQIVHAISYCHKLHVVHRDL
KPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVC
GQPPFQEANDSETLTMIMDCKYTVPSHVSKECKDLITRMLQRDPKRRAYLEEIENHPWLQGVDPSPATKY
NIPLVSYKNLSEEEHNSIIQRMVLGDIADRDAIVEALETNRYNHITATYFLLAERILREKQEKEIQTRSA
SPSNIKAQFRQSWPTKIDVPQDLEDDLTATPLSHATVPQSPARAADSVLNGHRSKGLCDSAKKDDLPELA
GPALSTVPPASLKPTASGRKCLFRVEEDEEEDEEDKKPMSLSTQVVLRRKPSVTNRLTSRKSAPVVNQIF
EEGESDDEFDMDENLPPKLSRLKMNIASPGTVHKRYHRRKSQGRGSSCSSSETSDDDSESRRRLDKDSGF
TYSWHRRDSSEGPPGSEGDGGGQSKPSNASGGVDKASPSENNAGGGSPSSGSGGNPTNTSGTTRRCAGPS
NSMQLASRSAGELVESLKLMSLCLGSQLHGSTKYIIDPQNGLSFSSVKVQEKSTWKMCISSTGNAGQVPA
VGGIKFFSDHMADTTTELERIRSKNLKNNVLQLPLCEKTISVNIQRNPKEGLLCASSPASCCHVI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_017719
ORF Size 2295 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_017719.5
RefSeq Size 5236 bp
RefSeq ORF 2298 bp
Locus ID 54861
UniProt ID Q9NRH2
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
MW 84.4 kDa
Gene Summary SNRK is a member of the sucrose nonfermenting (SNF)-related kinase family of serine/threonine kinases (Kertesz et al., 2002 [PubMed 12234663]).[supplied by OMIM, Apr 2009]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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