United States
Germany
Japan
United Kingdom
China
SNRK (NM_017719) Human Untagged Clone
CAT＃: SC323405
SNRK (untagged)-Kinase deficient mutant (K45M) of Human SNF related kinase (SNRK), transcript variant 1
CNY 5,608.00
CNY 7,220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HSNFRK |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323405 sequence for NM_017719 edited (data generated by NextGen Sequencing)
ATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGATTATATGATCTGGATAAA ACCTTGGGTCGAGGCCATTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGTGAA AAGGTGGCAGTAATGGTTATTGACAAGACAAAACTGGACACTCTAGCTACTGGTCATCTT TTCCAGGAAGTGAGATGCATGAAACTAGTGCAGCATCCTAACATCGTCCGCCTTTATGAA GTTATTGACACCCAGACCAAACTATATCTTATTCTAGAACTTGGGGATGGAGGAGATATG TTTGATTATATAATGAAACATGAGGAGGGTCTTAATGAAGACTTGGCCAAGAAGTATTTT GCTCAGATAGTTCATGCTATATCTTATTGCCATAAACTCCATGTGGTTCACAGAGACTTA AAACCAGAGAATGTAGTCTTCTTTGAAAAACAAGGTCTTGTAAAGTTGACAGACTTTGGG TTCAGCAACAAATTTCAACCAGGGAAGAAGCTCACTACAAGCTGTGGATCTCTTGCATAT TCCGCTCCAGAAATTCTGCTTGGTGATGAGTATGATGCACCTGCAGTAGATATTTGGAGT CTGGGAGTGATCCTTTTCATGTTGGTGTGTGGGCAGCCGCCCTTTCAAGAAGCCAATGAC AGTGAAACACTGACAATGATCATGGATTGCAAATATACAGTACCATCCCATGTGTCTAAA GAGTGTAAAGACCTAATCACACGGATGCTACAGAGAGATCCCAAGAGAAGGGCTTCTTTA GAAGAGATTGAAAATCATCCTTGGCTTCAGGGAGTGGACCCTTCACCAGCTACAAAGTAT AACATTCCCCTTGTGTCATACAAAAATCTCTCGGAAGAGGAGCACAACAGCATCATTCAG CGCATAGTGCTTGGGGACATAGCGGATCGAGACGCCATTGTAGAAGCCCTGGAAACCAAC AGGTATAACCATATCACAGCCACATACTTCCTGCTGGCTGAAAGGATCCTGAGAGAAAAG CAAGAGAAAGAAATACAGACCAGATCTGCAAGCCCGAGCAATATCAAGGCCCAGTTTAGG CAGTCATGGCCAACCAAAATTGATGTACCCCAGGACCTTGAGGATGACCTCACGGCCACT CCTTTGTCCCACGCGACTGTCCCTCAGTCTCCTGCTCGGGCTGCTGACAGTGTCCTCAAT GGCCACAGGAGCAAAGGCCTGTGTGACTCAGCTAAGAAAGATGACCTCCCTGAGTTGGCT GGACCAGCACTCTCTACGGTGCCACCCGCAAGCTTAAAACCCACAGCCAGTGGGCGGAAG TGTCTGTTCAGGGTGGAAGAAGATGAAGAGGAAGATGAGGAGGACAAGAAACCCATGTCC CTCTCAACACAAGTGGTTTTGCGCCGGAAGCCATCTGTAACCAACCGCCTGACATCCAGG AAGAGTGCGCCCGTCCTCAACCAGATCTTTGAGGAAGGGGAATCTGACGATGAGTTTGAC ATGGATGAGAATCTGCCTCCCAAGTTGAGCAGGTTAAAGATGAATATAGCTTCTCCAGGT ACAGTTCACAAACGCTACCACCGGAGGAAAAGTCAGGGCCGGGGCTCCAGCTGCAGTAGT TCGGAGACCAGTGATGATGATTCTGAAAGCCGGCGGCGGCTCGATAAAGATAGCGGGTTC ACCTACTCCTGGCACCGACGGGATAGCAGCGAGGGGCCCCCTGGCAGTGAGGGGGATGGC GGGGGCCAGAGCAAGCCAAGCAATGCCAGTGGAGGGGTGGACAAGGCCAGCCCCAGTGAG AACAATGCTGGTGGGGGCAGTCCCTCCAGCGGCTCGGGTGGCAACCCCACCAATACATCG GGTACCACACGCCGCTGTGCCGGCCCCAGCAACTCCATGCAGCTGGCCTCTCGCAGTGCT GGGGAGCTCGTTGAGAGCCTCAAACTCATGAGCCTCTGCCTCGGCTCCCAGCTTCATGGG AGCACCAAGTACATTATTGATCCACAGAATGGCTTGTCATTTTCCAGTGTGAAAGTCCAA GAGAAATCTACGTGGAAAATGTGCATTAGCTCCACAGGGAATGCAGGGCAGGTCCCTGCA GTGGGCGGCATAAAGTTTTTCTCTGACCACATGGCAGATACCACCACTGAATTGGAACGG ATAAAGAGCAAGAACCTGAAAAATAACGTGCTGCAGCTACCTCTGTGCGAAAAGACCATC TCTGTGAACATCCAGCGGAACCCTAAGGAGGGGCTGCTGTGCGCATCCAGCCCAGCCAGC TGTTGCCATGTCATCTGA Clone variation with respect to NM_017719.4 134 a=>t;135 a=>g;906 g=>a >OriGene 5' read for mutant NM_017719 unedited
CCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGAGTGGAGGTCTATATAAGCAGAGCTCGTTTAGTG AACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGACATTGAAAAT GAATTTTTTGTATTCACCAGATATTCTTATATGAGAAGATCTATTTTAAACAGTCTAAATATTTTTTCTT CTGTTGGACCAGCATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCTGGATTATATGATCTGGAT AAAACCTTGGGTCGAGGCCATTTTTGCCGTGGTTAAACTTGCCAGGCATGTCTTTACGGGGTGAAAGGTG TGTCGTGTAATGGTTATTGACAAGACACAAAACTGGACACTTCTACCTACTTGTTCATCCTTTTCCAGGA GTGGAGAAGGCTGGGAAACTATTGCCACCATTCCTAACTCCTTCCCGCCTTTTTAAATTTATTGACCCCC GAACCAACCTATTTCCTAATTCCAAAAACCTGGGGGAGGGAGGAAATTTTTTTTGTTTTTAAATGGAACC ATGGGAAAGGGTCTAATAAAAAATTGGGCAAAAAAGTTTTTTGCCCAAAAAGCCAGGCCTAACCCAAAGC GCCTAAACCCCGTGGGTTTCACAAAACCTAAACCCAGAAAGTGTTCTCTTTTTTAAAAAAGGGTTTTGAA ATTTCACAAATTTGGGTTCCCCCCATTTTCCCCCGGGGAAAACCCCAAAACCTGGGACTCTTTGATTTCC CCCCAAAATTTGCTGGGGAGAAATGAATGCCCCCCCGAAATTTGAGTCTGGAGTGTGCCCTTCTTGTGGT GGGAGCACCTTTTGAGACCGAGCGGAAACGCGAGATATTGTGTGGCATCAGGCCCCCGCGTGTGTAGTGT GAGACCTCCCCCGGCCTCCCCAAT >SC323405 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CKATGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGCGACATTGAAAATGAATTTT TTGTATTCACCAGATATTCTTATATGAGAAGATCTATTTTAAACAGTCTAAATATTTTTTCTTCTGTTGG ACCAGCATGGCAGGATTTAAGCGAGGGTATGATGGAAAGATTGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_017719 |
| Insert Size | 6220 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017719.3, NP_060189.2 |
| RefSeq Size | 5118 bp |
| RefSeq ORF | 2298 bp |
| Locus ID | 54861 |
| UniProt ID | Q9NRH2 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | SNRK is a member of the sucrose nonfermenting (SNF)-related kinase family of serine/threonine kinases (Kertesz et al., 2002 [PubMed 12234663]).[supplied by OMIM, Apr 2009] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same isoform (1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209726 | SNRK (Myc-DDK-tagged)-Human SNF related kinase (SNRK), transcript variant 1 |
CNY 5,600.00 |
|
| RC209726L1 | Lenti ORF clone of Human SNF related kinase (SNRK), transcript variant 1, Myc-DDK-tagged |
CNY 8,000.00 |
|
| RC209726L2 | Lenti ORF clone of Human SNF related kinase (SNRK), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC209726L3 | Lenti ORF clone of Human SNF related kinase (SNRK), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC209726L4 | Lenti ORF clone of Human SNF related kinase (SNRK), transcript variant 1, mGFP tagged |
CNY 8,000.00 |
|
| RG209726 | SNRK (tGFP-tagged) - Human SNF related kinase (SNRK), transcript variant 1 |
CNY 4,370.00 |
|
| SC114000 | SNRK (untagged)-Human SNF related kinase (SNRK), transcript variant 1 |
CNY 5,608.00 |
