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PDLIM2 (NM_021630) Human Tagged ORF Clone
CAT＃: RC210022
PDLIM2 (Myc-DDK-tagged)-Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2
ORF Plasmid: tGFP
"NM_021630" in other vectors (7)
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CNY 3,656.00
CNY 4,560.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MYSTIQUE; SLIM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210022 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTGACGGTGGATGTGGCCGGGCCAGCGCCCTGGGGCTTCCGTATCACAGGGGGCAGGGATTTCC ACACGCCCATCATGGTGACTAAGGTGGCCGAGCGGGGCAAAGCCAAGGACGCTGACCTCCGGCCTGGAGA CATAATCGTGGCCATCAACGGGGAAAGCGCGGAGGGCATGCTGCATGCCGAGGCCCAGAGCAAGATCCGC CAGAGCCCCTCGCCCCTGCGGCTGCAGCTGGACCGGTCTCAGGCTACGTCTCCAGGGCAGACCAATGGGG ACAGCTCCTTGGAAGTGCTGGCGACTCGCTTCCAGGGCTCCGTGAGGACATACACTGAGAGTCAGTCCTC CTTAAGGTCCTCCTACTCCAGCCCAACCTCCCTCAGCCCGAGGGCCGGCAGCCCCTTCTCACCACCACCC TCTAGCAGCTCCCTCACTGGAGAGGCGGCCATCAGCCGCAGCTTCCAGAGTCTGGCATGTTCCCCGGGCC TCCCCGCTGCTGACCGCCTGTCCTACTCAGGCCGCCCTGGAAGCCGACAGGCCGGCCTCGGCCGCGCTGG CGACTCGGCGGTGCTGGTGCTGCCGCCTTCCCCGGGCCCTCGTTCCTCCAGGCCCAGCATGGACTCGGAA GGGGGAAGCCTCCTCCTGGACGAGGACTCGGAAGTCTTCAAGATGCTGCAGGAAAATCGCGAGGGACGGG CGGCCCCCCGACAGTCCAGCTCCTTTCGGCTCTTGCAGGAAGCCCTGGAGGCTGAGGAGAGAGGTGGCAC GCCAGCCTTCTTGCCCAGCTCACTGAGCCCCCAGTCCTCCCTGCCCGCCTCCAGGGCCCTGGCCACCCCT CCCAAGCTCCACACTTGTGAGAAGTGCAGTACCAGCATCGCGAACCAGGCTGTGCGCATCCAGGAGGGCC GGTACCGCCACCCCGGCTGCTACACCTGTGCCGACTGTGGGCTGAACCTGAAGATGCGCGGGCACTTCTG GGTGGGTGACGAGCTGTACTGTGAGAAGCATGCCCGCCAGCGCTACTCCGCACCTGCCACCCTCAGCTCT CGGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210022 protein sequence
Red=Cloning site Green=Tags(s) MALTVDVAGPAPWGFRITGGRDFHTPIMVTKVAERGKAKDADLRPGDIIVAINGESAEGMLHAEAQSKIR QSPSPLRLQLDRSQATSPGQTNGDSSLEVLATRFQGSVRTYTESQSSLRSSYSSPTSLSPRAGSPFSPPP SSSSLTGEAAISRSFQSLACSPGLPAADRLSYSGRPGSRQAGLGRAGDSAVLVLPPSPGPRSSRPSMDSE GGSLLLDEDSEVFKMLQENREGRAAPRQSSSFRLLQEALEAEERGGTPAFLPSSLSPQSSLPASRALATP PKLHTCEKCSTSIANQAVRIQEGRYRHPGCYTCADCGLNLKMRGHFWVGDELYCEKHARQRYSAPATLSS RA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021630 |
| ORF Size | 1056 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021630.2 |
| RefSeq Size | 2246 bp |
| RefSeq ORF | 1809 bp |
| Locus ID | 64236 |
| UniProt ID | Q96JY6 |
| Domains | PDZ, LIM |
| MW | 37.5 kDa |
| Gene Summary | This gene encodes a member of the ALP subfamily of PDZ-LIM domain proteins. The encoded protein suppresses anchorage-dependent growth and promotes cell migration and adhesion through interactions with the actin cytoskeleton via the PDZ domain. The encoded protein is also a putative tumor suppressor protein, and decreased expression of this gene is associated with several malignancies including breast cancer and adult T-cell leukemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210022L1 | Lenti ORF clone of Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210022L2 | Lenti ORF clone of Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RC210022L3 | Lenti ORF clone of Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2, Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC210022L4 | Lenti ORF clone of Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2, mGFP tagged |
CNY 6,460.00 |
|
| RG210022 | PDLIM2 (tGFP-tagged) - Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2 |
CNY 5,040.00 |
|
| SC112905 | PDLIM2 (untagged)-Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2 |
CNY 3,656.00 |
|
| SC321005 | PDLIM2 (untagged)-Human PDZ and LIM domain 2 (mystique) (PDLIM2), transcript variant 2 |
CNY 3,656.00 |
