United States
Germany
Japan
United Kingdom
China
Serotonin transporter (SLC6A4) (NM_001045) Human Tagged ORF Clone
CAT＃: RC210187
SLC6A4 (Myc-DDK-tagged)-Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4)
ORF Plasmid: tGFP
"NM_001045" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 7,032.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HTT; 5-HTTLPR; 5HTT; hSERT; HTT; OCD1; SERT; SERT1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210187 representing NM_001045
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACGACGCCCTTGAATTCTCAGAAGCAGCTATCAGCGTGTGAAGATGGAGAAGATTGTCAGGAAA ACGGAGTTCTACAGAAGGTTGTTCCCACCCCAGGGGACAAAGTGGAGTCCGGGCAAATATCCAATGGGTA CTCAGCAGTTCCAAGTCCTGGTGCGGGAGATGACACACGGCACTCTATCCCAGCGACCACCACCACCCTA GTGGCTGAGCTTCATCAAGGGGAACGGGAGACCTGGGGCAAGAAGGTGGATTTCCTTCTCTCAGTGATTG GCTATGCTGTGGACCTGGGCAATGTCTGGCGCTTCCCCTACATATGTTACCAGAATGGAGGGGGGGCATT CCTCCTCCCCTACACCATCATGGCCATTTTTGGGGGAATCCCGCTCTTTTACATGGAGCTCGCACTGGGA CAGTACCACCGAAATGGATGCATTTCAATATGGAGGAAAATCTGCCCGATTTTCAAAGGGATTGGTTATG CCATCTGCATCATTGCCTTTTACATTGCTTCCTACTACAACACCATCATGGCCTGGGCGCTATACTACCT CATCTCCTCCTTCACGGACCAGCTGCCCTGGACCAGCTGCAAGAACTCCTGGAACACTGGCAACTGCACC AATTACTTCTCCGAGGACAACATCACCTGGACCCTCCATTCCACGTCCCCTGCTGAAGAATTTTACACGC GCCACGTCCTGCAGATCCACCGGTCTAAGGGGCTCCAGGACCTGGGGGGCATCAGCTGGCAGCTGGCCCT CTGCATCATGCTGATCTTCACTGTTATCTACTTCAGCATCTGGAAAGGCGTCAAGACCTCTGGCAAGGTG GTGTGGGTGACAGCCACCTTCCCTTATATCATCCTTTCTGTCCTGCTGGTGAGGGGTGCCACCCTCCCTG GAGCCTGGAGGGGTGTTCTCTTCTACTTGAAACCCAATTGGCAGAAACTCCTGGAGACAGGGGTGTGGAT AGATGCAGCCGCTCAGATCTTCTTCTCTCTTGGTCCGGGCTTTGGGGTCCTGCTGGCTTTTGCTAGCTAC AACAAGTTCAACAACAACTGCTACCAAGATGCCCTGGTGACCAGCGTGGTGAACTGCATGACGAGCTTCG TTTCGGGATTTGTCATCTTCACAGTGCTCGGTTACATGGCTGAGATGAGGAATGAAGATGTGTCTGAGGT GGCCAAAGACGCAGGTCCCAGCCTCCTCTTCATCACGTATGCAGAAGCGATAGCCAACATGCCAGCGTCC ACTTTCTTTGCCATCATCTTCTTTCTGATGTTAATCACGCTGGGCTTGGACAGCACGTTTGCAGGCTTGG AGGGGGTGATCACGGCTGTGCTGGATGAGTTCCCACACGTCTGGGCCAAGCGCCGGGAGCGGTTCGTGCT CGCCGTGGTCATCACCTGCTTCTTTGGATCCCTGGTCACCCTGACTTTTGGAGGGGCCTACGTGGTGAAG CTGCTGGAGGAGTATGCCACGGGGCCCGCAGTGCTCACTGTCGCGCTGATCGAAGCAGTCGCTGTGTCTT GGTTCTATGGCATCACTCAGTTCTGCAGGGACGTGAAGGAAATGCTCGGCTTCAGCCCGGGGTGGTTCTG GAGGATCTGCTGGGTGGCCATCAGCCCTCTGTTTCTCCTGTTCATCATTTGCAGTTTTCTGATGAGCCCG CCACAACTACGACTTTTCCAATATAATTATCCTTACTGGAGTATCATCTTGGGTTACTGCATAGGAACCT CATCTTTCATTTGCATCCCCACATATATAGCTTATCGGTTGATCATCACTCCAGGGACATTTAAAGAGCG TATTATTAAAAGTATTACCCCAGAAACACCAACAGAAATTCCTTGTGGGGACATCCGCTTGAATGCTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210187 representing NM_001045
Red=Cloning site Green=Tags(s) METTPLNSQKQLSACEDGEDCQENGVLQKVVPTPGDKVESGQISNGYSAVPSPGAGDDTRHSIPATTTTL VAELHQGERETWGKKVDFLLSVIGYAVDLGNVWRFPYICYQNGGGAFLLPYTIMAIFGGIPLFYMELALG QYHRNGCISIWRKICPIFKGIGYAICIIAFYIASYYNTIMAWALYYLISSFTDQLPWTSCKNSWNTGNCT NYFSEDNITWTLHSTSPAEEFYTRHVLQIHRSKGLQDLGGISWQLALCIMLIFTVIYFSIWKGVKTSGKV VWVTATFPYIILSVLLVRGATLPGAWRGVLFYLKPNWQKLLETGVWIDAAAQIFFSLGPGFGVLLAFASY NKFNNNCYQDALVTSVVNCMTSFVSGFVIFTVLGYMAEMRNEDVSEVAKDAGPSLLFITYAEAIANMPAS TFFAIIFFLMLITLGLDSTFAGLEGVITAVLDEFPHVWAKRRERFVLAVVITCFFGSLVTLTFGGAYVVK LLEEYATGPAVLTVALIEAVAVSWFYGITQFCRDVKEMLGFSPGWFWRICWVAISPLFLLFIICSFLMSP PQLRLFQYNYPYWSIILGYCIGTSSFICIPTYIAYRLIITPGTFKERIIKSITPETPTEIPCGDIRLNAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001045 |
| ORF Size | 1890 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001045.6 |
| RefSeq Size | 2756 bp |
| RefSeq ORF | 1893 bp |
| Locus ID | 6532 |
| UniProt ID | P31645 |
| Domains | SNF, 5HT_transporter |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 70.1 kDa |
| Gene Summary | This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake. There have been conflicting results in the literature about the possible effect, if any, that this polymorphism may play in behavior and depression. [provided by RefSeq, May 2019] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210187L1 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4), Myc-DDK-tagged |
CNY 9,432.00 |
|
| RC210187L2 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4), mGFP tagged |
CNY 5,890.00 |
|
| RC210187L3 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4), Myc-DDK-tagged |
CNY 9,432.00 |
|
| RC210187L4 | Lenti ORF clone of Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4), mGFP tagged |
CNY 9,432.00 |
|
| RG210187 | SLC6A4 (tGFP-tagged) - Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4) |
CNY 8,632.00 |
|
| SC119478 | SLC6A4 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, serotonin), member 4 (SLC6A4) |
CNY 7,048.00 |
其它Serotonin transporter产品
