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GPA33 (NM_005814) Human Tagged ORF Clone
CAT＃: RC210225
GPA33 (Myc-DDK-tagged)-Human glycoprotein A33 (transmembrane) (GPA33)
ORF Plasmid: tGFP
"NM_005814" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A33 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210225 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGGGGAAGATGTGGCCTGTGTTGTGGACACTCTGTGCAGTCAGGGTGACCGTCGATGCCATCTCTG TGGAAACTCCGCAGGACGTTCTTCGGGCTTCGCAGGGAAAGAGTGTCACCCTGCCCTGCACCTACCACAC TTCCACCTCCAGTCGAGAGGGACTTATTCAATGGGATAAGCTCCTCCTCACTCATACGGAAAGGGTGGTC ATCTGGCCGTTTTCAAACAAAAACTACATCCATGGTGAGCTTTATAAGAATCGCGTCAGCATATCCAACA ATGCTGAGCAGTCCGATGCCTCCATCACCATTGATCAGCTGACCATGGCTGACAACGGCACCTACGAGTG TTCTGTCTCGCTGATGTCAGACCTGGAGGGCAACACCAAGTCACGTGTCCGCCTGTTGGTCCTCGTGCCA CCCTCCAAACCAGAATGCGGCATCGAGGGAGAGACCATAATTGGGAACAACATCCAGCTGACCTGCCAAT CAAAGGAGGGCTCACCAACCCCTCAGTACAGCTGGAAGAGGTACAACATCCTGAATCAGGAGCAGCCCCT GGCCCAGCCAGCCTCAGGTCAGCCTGTCTCCCTGAAGAATATCTCCACAGACACATCGGGTTACTACATC TGTACCTCCAGCAATGAGGAGGGGACGCAGTTCTGCAACATCACGGTGGCCGTCAGATCTCCCTCCATGA ACGTGGCCCTGTATGTGGGCATCGCGGTGGGCGTGGTTGCAGCCCTCATTATCATTGGCATCATCATCTA CTGCTGCTGCTGCCGAGGGAAGGACGACAACACTGAAGACAAGGAGGATGCAAGGCCGAACCGGGAAGCC TATGAGGAGCCACCAGAGCAGCTAAGAGAACTTTCCAGAGAGAGGGAGGAGGAGGATGACTACAGGCAAG AAGAGCAGAGGAGCACTGGGCGTGAATCCCCGGACCACCTCGACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210225 protein sequence
Red=Cloning site Green=Tags(s) MVGKMWPVLWTLCAVRVTVDAISVETPQDVLRASQGKSVTLPCTYHTSTSSREGLIQWDKLLLTHTERVV IWPFSNKNYIHGELYKNRVSISNNAEQSDASITIDQLTMADNGTYECSVSLMSDLEGNTKSRVRLLVLVP PSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYI CTSSNEEGTQFCNITVAVRSPSMNVALYVGIAVGVVAALIIIGIIIYCCCCRGKDDNTEDKEDARPNREA YEEPPEQLRELSREREEEDDYRQEEQRSTGRESPDHLDQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005814 |
| ORF Size | 957 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005814.3 |
| RefSeq Size | 2793 bp |
| RefSeq ORF | 960 bp |
| Locus ID | 10223 |
| UniProt ID | Q99795 |
| Protein Families | Druggable Genome, Transmembrane |
| MW | 35.6 kDa |
| Gene Summary | The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210225L1 | Lenti ORF clone of Human glycoprotein A33 (transmembrane) (GPA33), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210225L2 | Lenti ORF clone of Human glycoprotein A33 (transmembrane) (GPA33), mGFP tagged |
CNY 5,890.00 |
|
| RC210225L3 | Lenti ORF clone of Human glycoprotein A33 (transmembrane) (GPA33), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC210225L4 | Lenti ORF clone of Human glycoprotein A33 (transmembrane) (GPA33), mGFP tagged |
CNY 5,890.00 |
|
| RG210225 | GPA33 (tGFP-tagged) - Human glycoprotein A33 (transmembrane) (GPA33) |
CNY 4,000.00 |
|
| SC303695 | GPA33 (untagged)-Human glycoprotein A33 (transmembrane) (GPA33) |
CNY 2,400.00 |
