AGPAT5 (NM_018361) Human Tagged ORF Clone
CAT#: RC210280
- TrueORF®
AGPAT5 (Myc-DDK-tagged)-Human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5)
ORF Plasmid: tGFP
"NM_018361" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1AGPAT5; LPAATE |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC210280 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTGTCCCTGGTGCTCCACACGTACTCCATGCGCTACCTGCTGCCCAGCGTCGTGCTCCTGGGCA CGGCGCCCACCTACGTGTTGGCCTGGGGGGTCTGGCGGCTGCTCTCCGCCTTCCTGCCCGCCCGCTTCTA CCAAGCGCTGGACGACCGGCTCTACTGCGTCTACCAGAGCATGGTGCTCTTCTTCTTCGAGAATTACACC GGGGTCCAGATATTGCTATATGGAGATTTGCCAAAAAATAAAGAAAATATAATATATTTAGCAAATCATC AAAGCACAGTTGACTGGATTGTTGCTGACATCTTGGCCATCAGGCAGAATGCGCTAGGACATGTGCGCTA CGTGCTGAAAGAAGGGTTAAAATGGCTGCCATTGTATGGGTGTTACTTTGCTCAGCATGGAGGAATCTAT GTAAAGCGCAGTGCCAAATTTAACGAGAAAGAGATGCGAAACAAGTTGCAGAGCTACGTGGACGCAGGAA CTCCAATGTATCTTGTGATTTTTCCAGAAGGTACAAGGTATAATCCAGAGCAAACAAAAGTCCTTTCAGC TAGTCAGGCATTTGCTGCCCAACGTGGCCTTGCAGTATTAAAACATGTGCTAACACCACGAATAAAGGCA ACTCACGTTGCTTTTGATTGCATGAAGAATTATTTAGATGCAATTTATGATGTTACGGTGGTTTATGAAG GGAAAGACGATGGAGGGCAGCGAAGAGAGTCACCGACCATGACGGAATTTCTCTGCAAAGAATGTCCAAA AATTCATATTCACATTGATCGTATCGACAAAAAAGATGTCCCAGAAGAACAAGAACATATGAGAAGATGG CTGCATGAACGTTTCGAAATCAAAGATAAGATGCTTATAGAATTTTATGAGTCACCAGATCCAGAAAGAA GAAAAAGATTTCCTGGGAAAAGTGTTAATTCCAAATTAAGTATCAAGAAGACTTTACCATCAATGTTGAT CTTAAGTGGTTTGACTGCAGGCATGCTTATGACCGATGCTGGAAGGAAGCTGTATGTGAACACCTGGATA TATGGAACCCTACTTGGCTGCCTGTGGGTTACTATTAAAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210280 protein sequence
Red=Cloning site Green=Tags(s) MLLSLVLHTYSMRYLLPSVVLLGTAPTYVLAWGVWRLLSAFLPARFYQALDDRLYCVYQSMVLFFFENYT GVQILLYGDLPKNKENIIYLANHQSTVDWIVADILAIRQNALGHVRYVLKEGLKWLPLYGCYFAQHGGIY VKRSAKFNEKEMRNKLQSYVDAGTPMYLVIFPEGTRYNPEQTKVLSASQAFAAQRGLAVLKHVLTPRIKA THVAFDCMKNYLDAIYDVTVVYEGKDDGGQRRESPTMTEFLCKECPKIHIHIDRIDKKDVPEEQEHMRRW LHERFEIKDKMLIEFYESPDPERRKRFPGKSVNSKLSIKKTLPSMLILSGLTAGMLMTDAGRKLYVNTWI YGTLLGCLWVTIKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_018361 |
ORF Size | 1092 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_018361.5 |
RefSeq Size | 5535 bp |
RefSeq ORF | 1095 bp |
Locus ID | 55326 |
UniProt ID | Q9NUQ2 |
Domains | Acyltransferase |
Protein Families | Transmembrane |
MW | 42.1 kDa |
Gene Summary | This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. A pseudogene of this gene is present on the Y chromosome. [provided by RefSeq, Aug 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210280L3 | Lenti ORF clone of Human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210280L4 | Lenti ORF clone of Human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5), mGFP tagged |
CNY 5,890.00 |
|
RG210280 | AGPAT5 (tGFP-tagged) - Human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) |
CNY 5,256.00 |
|
SC319824 | AGPAT5 (untagged)-Human 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) (AGPAT5) |
CNY 3,656.00 |