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B7H4 (VTCN1) (NM_024626) Human Tagged ORF Clone
CAT＃: RC210360
VTCN1 (Myc-DDK-tagged)-Human V-set domain containing T cell activation inhibitor 1 (VTCN1)
ORF Plasmid: tGFP
"NM_024626" in other vectors (6)
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CNY 3,600.00
| Cited in 2 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B7-H4; B7h.5; B7H4; B7S1; B7X; PRO1291; VCTN1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210360 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCCTGGGGCAGATCCTCTTCTGGAGCATAATTAGCATCATCATTATTCTGGCTGGAGCAATTG CACTCATCATTGGCTTTGGTATTTCAGGGAGACACTCCATCACAGTCACTACTGTCGCCTCAGCTGGGAA CATTGGGGAGGATGGAATCCTGAGCTGCACTTTTGAACCTGACATCAAACTTTCTGATATCGTGATACAA TGGCTGAAGGAAGGTGTTTTAGGCTTGGTCCATGAGTTCAAAGAAGGCAAAGATGAGCTGTCGGAGCAGG ATGAAATGTTCAGAGGCCGGACAGCAGTGTTTGCTGATCAAGTGATAGTTGGCAATGCCTCTTTGCGGCT GAAAAACGTGCAACTCACAGATGCTGGCACCTACAAATGTTATATCATCACTTCTAAAGGCAAGGGGAAT GCTAACCTTGAGTATAAAACTGGAGCCTTCAGCATGCCGGAAGTGAATGTGGACTATAATGCCAGCTCAG AGACCTTGCGGTGTGAGGCTCCCCGATGGTTCCCCCAGCCCACAGTGGTCTGGGCATCCCAAGTTGACCA GGGAGCCAACTTCTCGGAAGTCTCCAATACCAGCTTTGAGCTGAACTCTGAGAATGTGACCATGAAGGTT GTGTCTGTGCTCTACAATGTTACGATCAACAACACATACTCCTGTATGATTGAAAATGACATTGCCAAAG CAACAGGGGATATCAAAGTGACAGAATCGGAGATCAAAAGGCGGAGTCACCTACAGCTGCTAAACTCAAA GGCTTCTCTGTGTGTCTCTTCTTTCTTTGCCATCAGCTGGGCACTTCTGCCTCTCAGCCCTTACCTGATG CTAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210360 protein sequence
Red=Cloning site Green=Tags(s) MASLGQILFWSIISIIIILAGAIALIIGFGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQ WLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGN ANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKV VSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKASLCVSSFFAISWALLPLSPYLM LK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024626 |
| ORF Size | 846 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024626.4 |
| RefSeq Size | 2652 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 79679 |
| UniProt ID | Q7Z7D3 |
| Protein Families | Transmembrane |
| MW | 30.9 kDa |
| Gene Summary | This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
B7-H4 overexpression contributes to poor prognosis and drug-resistance in triple-negative breast cancer
,Wang, L;Yang, C;Liu, XB;Wang, L;Kang, FB;,
Cancer Cell Int.
,PubMed ID 30008617
[B7H4]
|
|
The tissue distribution and significance of B7-H4 in laryngeal carcinoma
,Chen, L;Jin, M;Li, C;Shang, Y;Zhang, Q;,
Oncotarget
,PubMed ID 29190910
[B7H4]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210360L1 | Lenti ORF clone of Human V-set domain containing T cell activation inhibitor 1 (VTCN1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC210360L2 | Lenti ORF clone of Human V-set domain containing T cell activation inhibitor 1 (VTCN1), mGFP tagged |
CNY 6,000.00 |
|
| RC210360L3 | Lenti ORF clone of Human V-set domain containing T cell activation inhibitor 1 (VTCN1), Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC210360L4 | Lenti ORF clone of Human V-set domain containing T cell activation inhibitor 1 (VTCN1), mGFP tagged |
CNY 6,000.00 |
|
| RG210360 | VTCN1 (tGFP-tagged) - Human V-set domain containing T cell activation inhibitor 1 (VTCN1) |
CNY 5,200.00 |
|
| SC112129 | VTCN1 (untagged)-Human V-set domain containing T cell activation inhibitor 1 (VTCN1) |
CNY 3,600.00 |
