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GRPR (NM_005314) Human Tagged ORF Clone
CAT＃: RC210362
GRPR (Myc-DDK-tagged)-Human gastrin-releasing peptide receptor (GRPR)
ORF Plasmid: tGFP
"NM_005314" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BB2; BB2R; BRS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTAAATGACTGTTTCCTTCTGAACTTGGAGGTGGACCATTTCATGCACTGCAACATCTCCAGTC ACAGTGCGGATCTCCCCGTGAACGATGACTGGTCCCACCCGGGGATCCTCTATGTCATCCCTGCAGTTTA TGGGGTTATCATTCTGATAGGCCTCATTGGCAACATCACTTTGATCAAGATCTTCTGTACAGTCAAGTCC ATGCGAAACGTTCCAAACCTGTTCATTTCCAGTCTGGCTTTGGGAGACCTGCTCCTCCTAATAACGTGTG CTCCAGTGGATGCCAGCAGGTACCTGGCTGACAGATGGCTATTTGGCAGGATTGGCTGCAAACTGATCCC CTTTATACAGCTTACCTCTGTTGGGGTGTCTGTCTTCACACTCACGGCGCTCTCGGCAGACAGATACAAA GCCATTGTCCGGCCAATGGATATCCAGGCCTCCCATGCCCTGATGAAGATCTGCCTCAAAGCCGCCTTTA TCTGGATCATCTCCATGCTGCTGGCCATTCCAGAGGCCGTGTTTTCTGACCTCCATCCCTTCCATGAGGA AAGCACCAACCAGACCTTCATTAGCTGTGCCCCATACCCACACTCTAATGAGCTTCACCCCAAAATCCAT TCTATGGCTTCCTTTCTGGTCTTCTACGTCATCCCACTGTCGATCATCTCTGTTTACTACTACTTCATTG CTAAAAATCTGATCCAGAGTGCTTACAATCTTCCCGTGGAAGGGAATATACATGTCAAGAAGCAGATTGA ATCCCGGAAGCGACTTGCCAAGACAGTGCTGGTGTTTGTGGGCCTGTTCGCCTTCTGCTGGCTCCCCAAT CATGTCATCTACCTGTACCGCTCCTACCACTACTCTGAGGTGGACACCTCCATGCTCCACTTTGTCACCA GCATCTGTGCCCGCCTCCTGGCCTTCACCAACTCCTGCGTGAACCCCTTTGCCCTCTACCTGCTGAGCAA GAGTTTCAGGAAACAGTTCAACACTCAGCTGCTCTGTTGCCAGCCTGGCCTGATCATCCGGTCTCACAGC ACTGGAAGGAGTACAACCTGCATGACCTCCCTCAAGAGTACCAACCCCTCCGTGGCCACCTTTAGCCTCA TCAATGGAAACATCTGTCACGAGCGGTATGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210362 protein sequence
Red=Cloning site Green=Tags(s) MALNDCFLLNLEVDHFMHCNISSHSADLPVNDDWSHPGILYVIPAVYGVIILIGLIGNITLIKIFCTVKS MRNVPNLFISSLALGDLLLLITCAPVDASRYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYK AIVRPMDIQASHALMKICLKAAFIWIISMLLAIPEAVFSDLHPFHEESTNQTFISCAPYPHSNELHPKIH SMASFLVFYVIPLSIISVYYYFIAKNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPN HVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLIIRSHS TGRSTTCMTSLKSTNPSVATFSLINGNICHERYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005314 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005314.1 |
| RefSeq Size | 2681 bp |
| RefSeq ORF | 1155 bp |
| Locus ID | 2925 |
| UniProt ID | P30550 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Neuroactive ligand-receptor interaction |
| MW | 43.2 kDa |
| Gene Summary | Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A human GRPr-transfected Ace-1 canine prostate cancer model in mice
,Ding, H;Kothandaraman, S;Gong, L;Williams, MM;Dirksen, WP;Rosol, TJ;Tweedle, MF;,
Prostate
,PubMed ID 26940014
[GRPR]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210362L1 | Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210362L2 | Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), mGFP tagged |
CNY 5,890.00 |
|
| RC210362L3 | Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210362L4 | Lenti ORF clone of Human gastrin-releasing peptide receptor (GRPR), mGFP tagged |
CNY 5,890.00 |
|
| RG210362 | GRPR (tGFP-tagged) - Human gastrin-releasing peptide receptor (GRPR) |
CNY 5,256.00 |
|
| SC116819 | GRPR (untagged)-Human gastrin-releasing peptide receptor (GRPR) |
CNY 3,656.00 |
