Opticin (OPTC) (NM_014359) Human Tagged ORF Clone

CAT#: RC210384

OPTC (Myc-DDK-tagged)-Human opticin (OPTC)

ORF Plasmid: DDK tGFP



  "NM_014359" in other vectors (6)

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CNY 2,400.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-OPTC Antibody
    • 100 ul

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms OPT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210384 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCTCCTGGCTTTCCTGAGTCTGCTGGCCTTGGTGCTGCAGGAGACAGGGACAGCTTCTCTCCCAA
GGAAGGAGAGGAAGAGGAGAGAGGAGCAGATGCCCAGGGAAGGCGATTCCTTTGAAGTTCTGCCTCTGCG
GAATGATGTCCTGAACCCAGACAACTATGGTGAAGTCATTGACCTGAGCAACTATGAGGAGCTCACAGAT
TATGGGGACCAACTCCCCGAGGTTAAGGTGACTAGCCTCGCTCCTGCAACCAGCATCAGTCCCGCCAAGA
GCACTACGGCTCCAGGGACACCCTCGTCAAACCCCACGATGACCAGACCTACTACAGCAGGGCTGCTACT
GAGTTCCCAGCCCAACCATGGTCTGCCCACCTGCCTGGTCTGCGTGTGCCTCGGTTCCTCTGTGTATTGC
GATGACATTGACCTAGAGGACATTCCTCCTCTTCCTCGGAGGACTGCCTACCTGTATGCACGCTTCAACC
GCATCAGCCGTATCAGGGCCGAAGACTTCAAAGGGCTGACAAAGTTGAAGAGGATTGACCTCTCCAACAA
CCTCATTTCCTCCATCGATAATGATGCCTTCCGCCTGCTACATGCCCTCCAGGACCTCATCCTCCCAGAG
AACCAGTTGGAAGCTCTGCCCGTGCTGCCCAGTGGCATTGAGTTCCTGGATGTCCGCCTAAATCGGCTCC
AGAGCTCGGGGATACAGCCTGCAGCCTTCAGGGCAATGGAGAAGCTGCAGTTCCTTTACCTGTCAGACAA
CCTGCTGGATTCTATCCCGGGGCCTTTGCCCCTGAGCCTGCGCTCTGTACACCTGCAGAATAACCTGATA
GAGACCATGCAGAGAGACGTCTTCTGTGACCCCGAGGAGCACAAACACACCCGCAGGCAGCTGGAAGACA
TCCGCCTGGATGGCAACCCCATCAACCTCAGCCTCTTCCCCAGCGCCTACTTCTGCCTGCCTCGGCTCCC
CATCGGCCGCTTCACG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210384 protein sequence
Red=Cloning site Green=Tags(s)

MRLLAFLSLLALVLQETGTASLPRKERKRREEQMPREGDSFEVLPLRNDVLNPDNYGEVIDLSNYEELTD
YGDQLPEVKVTSLAPATSISPAKSTTAPGTPSSNPTMTRPTTAGLLLSSQPNHGLPTCLVCVCLGSSVYC
DDIDLEDIPPLPRRTAYLYARFNRISRIRAEDFKGLTKLKRIDLSNNLISSIDNDAFRLLHALQDLILPE
NQLEALPVLPSGIEFLDVRLNRLQSSGIQPAAFRAMEKLQFLYLSDNLLDSIPGPLPLSLRSVHLQNNLI
ETMQRDVFCDPEEHKHTRRQLEDIRLDGNPINLSLFPSAYFCLPRLPIGRFT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014359
ORF Size 996 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014359.4
RefSeq Size 1444 bp
RefSeq ORF 999 bp
Locus ID 26254
UniProt ID Q9UBM4
Protein Families Secreted Protein
MW 37.3 kDa
Gene Summary Opticin belongs to class III of the small leucine-rich repeat protein (SLRP) family. Members of this family are typically associated with the extracellular matrix. Opticin is present in significant quantities in the vitreous of the eye and also localizes to the cornea, iris, ciliary body, optic nerve, choroid, retina, and fetal liver. Opticin may noncovalently bind collagen fibrils and regulate fibril morphology, spacing, and organization. The opticin gene is mapped to a region of chromosome 1 that is associated with the inherited eye diseases age-related macular degeneration (AMD) and posterior column ataxia with retinosa pigmentosa (AXPC1). [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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