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Aminopeptidase A (ENPEP) (NM_001977) Human Tagged ORF Clone
CAT＃: RC210521
ENPEP (Myc-DDK-tagged)-Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP)
ORF Plasmid: tGFP
"NM_001977" in other vectors (4)
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CNY 7,008.00
CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | APA; CD249; gp160 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210521 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTGCGGAGAGAGAGGGCTCTAAGAGATACTGCATTCAAACGAAACATGTGGCCATTCTCTGTG CGGTGGTGGTGGGTGTAGGATTAATAGTGGGACTTGCCGTGGGCTTGACCAGATCGTGTGACTCCAGCGG GGACGGCGGGCCGGGCACTGCGCCAGCTCCTTCCCACCTGCCTTCTTCCACGGCCAGCCCCTCAGGTCCT CCTGCCCAGGACCAGGACATCTGCCCGGCCAGTGAGGATGAGAGCGGACAGTGGAAAAACTTTCGACTGC CGGACTTCGTCAACCCAGTCCACTACGACCTGCACGTGAAGCCCCTGTTGGAGGAGGACACCTACACGGG CACCGTGAGCATCTCCATCAACCTGAGCGCTCCCACCCGGTACCTGTGGCTGCACCTCCGGGAGACCAGG ATCACCCGGCTCCCGGAGCTGAAGAGGCCCTCTGGGGACCAGGTGCAAGTCCGGAGGTGTTTCGAGTACA AAAAGCAGGAGTACGTGGTGGTCGAGGCGGAGGAAGAGCTTACCCCCAGCAGTGGAGATGGCCTGTATCT CCTGACCATGGAGTTCGCCGGCTGGCTGAACGGCTCCCTCGTGGGATTTTATAGAACCACCTACACGGAG AACGGACGAGTCAAGAGCATAGTGGCCACCGATCATGAACCAACAGATGCCAGGAAATCTTTTCCTTGTT TTGATGAGCCCAACAAAAAGGCAACTTATACAATATCTATCACCCATCCCAAAGAATACGGAGCACTTTC AAATATGCCAGTGGCGAAAGAAGAGTCAGTGGATGATAAATGGACTCGAACAACTTTTGAGAAGTCTGTC CCCATGAGCACGTACCTGGTGTGCTTTGCTGTACATCAATTTGACTCTGTAAAGAGAATATCAAATAGTG GAAAACCTCTTACAATTTATGTCCAGCCAGAGCAAAAGCACACAGCCGAATATGCTGCAAACATAACTAA AAGTGTGTTTGATTATTTTGAAGAATACTTTGCTATGAATTATTCTCTTCCTAAATTAGATAAAATCGCT ATTCCAGATTTTGGCACTGGTGCCATGGAGAACTGGGGACTCATCACGTACAGAGAAACGAACCTGCTTT ATGACCCTAAGGAATCAGCCTCATCAAACCAACAGAGGGTGGCCACTGTGGTTGCCCATGAACTTGTGCA TCAGTGGTTTGGAAATATTGTGACCATGGACTGGTGGGAAGACTTGTGGCTAAATGAAGGATTTGCTTCT TTCTTTGAGTTTCTGGGAGTAAACCATGCAGAAACAGACTGGCAAATGCGTGACCAAATGTTACTTGAAG ATGTATTACCTGTTCAAGAGGATGATTCTTTGATGTCTTCGCATCCAATTATTGTGACTGTGACAACCCC TGATGAAATAACATCTGTTTTTGATGGAATATCCTATAGCAAGGGATCTTCTATTTTGAGAATGCTTGAA GACTGGATAAAACCAGAGAATTTTCAAAAAGGATGTCAGATGTACTTGGAAAAATACCAATTCAAGAATG CAAAAACTTCTGATTTTTGGGCAGCACTGGAAGAGGCAAGTAGGCTACCAGTGAAAGAAGTAATGGACAC CTGGACCAGACAGATGGGTTATCCTGTGCTTAACGTGAACGGTGTCAAGAACATCACACAGAAACGCTTT TTGTTGGACCCAAGAGCTAACCCTTCTCAGCCCCCTTCAGATCTTGGTTATACATGGAATATCCCAGTTA AATGGACTGAAGATAATATAACAAGCAGTGTGTTATTTAATAGGTCAGAAAAAGAAGGAATCACTTTGAA CTCCTCTAATCCTAGTGGAAATGCTTTTCTCAAAATAAACCCAGATCATATTGGGTTTTATCGTGTAAAT TATGAAGTAGCAACTTGGGACTCGATAGCTACAGCGCTCTCCTTGAACCACAAGACATTTTCTTCAGCAG ATCGTGCAAGTCTTATTGATGATGCTTTTGCCTTGGCAAGAGCTCAACTTCTAGATTATAAGGTGGCTTT GAACTTGACCAAGTATCTCAAAAGGGAAGAGAATTTTTTACCATGGCAGAGAGTAATTTCAGCTGTAACC TACATCATTAGCATGTTTGAAGATGATAAAGAGCTATATCCTATGATTGAGGAATACTTCCAAGGTCAAG TGAAGCCTATTGCAGATTCTCTGGGATGGAATGATGCTGGAGACCATGTCACAAAGTTACTCCGTTCCTC CGTGTTAGGGTTTGCGTGCAAGATGGGAGACAGAGAAGCCTTGAACAATGCTTCCTCGTTATTTGAGCAG TGGCTAAATGGGACTGTAAGCCTTCCCGTAAATCTCAGGCTTCTGGTGTATCGGTATGGGATGCAGAACT CTGGCAATGAGATTTCATGGAACTACACTCTTGAGCAATACCAGAAAACTTCATTAGCTCAAGAAAAAGA AAAACTGCTGTATGGATTAGCATCAGTGAAGAACGTTACTCTTTTGTCAAGGTATTTGGATTTGCTCAAG GACACGAACCTTATTAAAACTCAGGATGTGTTTACAGTCATTCGATATATCTCATATAACAGCTATGGGA AGAACATGGCCTGGAATTGGATACAACTCAACTGGGACTATCTAGTCAACAGATATACACTCAATAACAG AAACCTTGGCCGAATTGTCACAATAGCAGAGCCATTCAACACTGAACTGCAACTGTGGCAGATGGAGAGC TTTTTTGCAAAATATCCACAAGCTGGAGCAGGAGAAAAACCTAGGGAACAAGTGCTGGAAACAGTGAAAA ACAATATAGAGTGGCTAAAACAACATAGAAACACCATCAGAGAATGGTTTTTTAATTTACTTGAGAGTGG T ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210521 protein sequence
Red=Cloning site Green=Tags(s) MNFAEREGSKRYCIQTKHVAILCAVVVGVGLIVGLAVGLTRSCDSSGDGGPGTAPAPSHLPSSTASPSGP PAQDQDICPASEDESGQWKNFRLPDFVNPVHYDLHVKPLLEEDTYTGTVSISINLSAPTRYLWLHLRETR ITRLPELKRPSGDQVQVRRCFEYKKQEYVVVEAEEELTPSSGDGLYLLTMEFAGWLNGSLVGFYRTTYTE NGRVKSIVATDHEPTDARKSFPCFDEPNKKATYTISITHPKEYGALSNMPVAKEESVDDKWTRTTFEKSV PMSTYLVCFAVHQFDSVKRISNSGKPLTIYVQPEQKHTAEYAANITKSVFDYFEEYFAMNYSLPKLDKIA IPDFGTGAMENWGLITYRETNLLYDPKESASSNQQRVATVVAHELVHQWFGNIVTMDWWEDLWLNEGFAS FFEFLGVNHAETDWQMRDQMLLEDVLPVQEDDSLMSSHPIIVTVTTPDEITSVFDGISYSKGSSILRMLE DWIKPENFQKGCQMYLEKYQFKNAKTSDFWAALEEASRLPVKEVMDTWTRQMGYPVLNVNGVKNITQKRF LLDPRANPSQPPSDLGYTWNIPVKWTEDNITSSVLFNRSEKEGITLNSSNPSGNAFLKINPDHIGFYRVN YEVATWDSIATALSLNHKTFSSADRASLIDDAFALARAQLLDYKVALNLTKYLKREENFLPWQRVISAVT YIISMFEDDKELYPMIEEYFQGQVKPIADSLGWNDAGDHVTKLLRSSVLGFACKMGDREALNNASSLFEQ WLNGTVSLPVNLRLLVYRYGMQNSGNEISWNYTLEQYQKTSLAQEKEKLLYGLASVKNVTLLSRYLDLLK DTNLIKTQDVFTVIRYISYNSYGKNMAWNWIQLNWDYLVNRYTLNNRNLGRIVTIAEPFNTELQLWQMES FFAKYPQAGAGEKPREQVLETVKNNIEWLKQHRNTIREWFFNLLESG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001977 |
| ORF Size | 2871 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001977.2, NP_001968.2 |
| RefSeq Size | 5004 bp |
| RefSeq ORF | 2874 bp |
| Locus ID | 2028 |
| UniProt ID | Q07075 |
| Domains | Peptidase_M1 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane |
| Protein Pathways | Renin-angiotensin system |
| MW | 109.3 kDa |
| Gene Summary | The ENPEP gene encodes glutamyl aminopeptidase, a type II integral membrane protein with an extracellular zinc-binding domain. This protein can upregulate blood pressure by cleaving the N-terminal aspartate from angiotensin II, and can regulate blood vessel formation and enhance tumorigenesis in some tissues. Along with ANPEP and DPP4, ENPEP was found to be a candidate co-receptor for the coronavirus SARS-CoV-2, which causes COVID-19. [provided by RefSeq, Apr 2020] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210521L3 | Lenti ORF clone of Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC210521L4 | Lenti ORF clone of Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP), mGFP tagged |
CNY 9,030.00 |
|
| RG210521 | ENPEP (tGFP-tagged) - Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP) |
CNY 7,890.00 |
|
| SC110882 | ENPEP (untagged)-Human glutamyl aminopeptidase (aminopeptidase A) (ENPEP) |
CNY 7,016.00 |
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