United States
Germany
Japan
United Kingdom
China
Isocitrate dehydrogenase (IDH1) (NM_005896) Human Tagged ORF Clone
CAT＃: RC210582
IDH1 (Myc-DDK-tagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1)
ORF Plasmid: tGFP
"NM_005896" in other vectors (7)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
| Cited in 7 publications. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210582 representing NM_005896
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAAAAAAATCAGTGGCGGTTCTGTGGTAGAGATGCAAGGAGATGAAATGACACGAATCATTTGGG AATTGATTAAAGAGAAACTCATTTTTCCCTACGTGGAATTGGATCTACATAGCTATGATTTAGGCATAGA GAATCGTGATGCCACCAACGACCAAGTCACCAAGGATGCTGCAGAAGCTATAAAGAAGCATAATGTTGGC GTCAAATGTGCCACTATCACTCCTGATGAGAAGAGGGTTGAGGAGTTCAAGTTGAAACAAATGTGGAAAT CACCAAATGGCACCATACGAAATATTCTGGGTGGCACGGTCTTCAGAGAAGCCATTATCTGCAAAAATAT CCCCCGGCTTGTGAGTGGATGGGTAAAACCTATCATCATAGGTCGTCATGCTTATGGGGATCAATACAGA GCAACTGATTTTGTTGTTCCTGGGCCTGGAAAAGTAGAGATAACCTACACACCAAGTGACGGAACCCAAA AGGTGACATACCTGGTACATAACTTTGAAGAAGGTGGTGGTGTTGCCATGGGGATGTATAATCAAGATAA GTCAATTGAAGATTTTGCACACAGTTCCTTCCAAATGGCTCTGTCTAAGGGTTGGCCTTTGTATCTGAGC ACCAAAAACACTATTCTGAAGAAATATGATGGGCGTTTTAAAGACATCTTTCAGGAGATATATGACAAGC AGTACAAGTCCCAGTTTGAAGCTCAAAAGATCTGGTATGAGCATAGGCTCATCGACGACATGGTGGCCCA AGCTATGAAATCAGAGGGAGGCTTCATCTGGGCCTGTAAAAACTATGATGGTGACGTGCAGTCGGACTCT GTGGCCCAAGGGTATGGCTCTCTCGGCATGATGACCAGCGTGCTGGTTTGTCCAGATGGCAAGACAGTAG AAGCAGAGTCTGCCCACGGGACTGTAACCCGTCACTACCGCATGTACCAGAAAGGACAGGAGACGTCCAC CAATCCCATTGCTTCCATTTTTGCCTGGACCAGAGGGTTAGCCCACAGAGCAAAGCTTGATAACAATAAA GAGCTTGCCTTCTTTGCAAATGCTTTGGAAGAAGTCTCTATTGAGACAATTGAGGCTGGCTTCATGACCA AGGACTTGGCTGCTTGCATTAAAGGTTTACCCAATGTGCAACGTTCTGACTACTTGAATACATTTGAGTT CATGGATAAACTTGGAGAAAACTTGAAGATCAAACTAGCTCAGGCCAAACTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210582 representing NM_005896
Red=Cloning site Green=Tags(s) MSKKISGGSVVEMQGDEMTRIIWELIKEKLIFPYVELDLHSYDLGIENRDATNDQVTKDAAEAIKKHNVG VKCATITPDEKRVEEFKLKQMWKSPNGTIRNILGGTVFREAIICKNIPRLVSGWVKPIIIGRHAYGDQYR ATDFVVPGPGKVEITYTPSDGTQKVTYLVHNFEEGGGVAMGMYNQDKSIEDFAHSSFQMALSKGWPLYLS TKNTILKKYDGRFKDIFQEIYDKQYKSQFEAQKIWYEHRLIDDMVAQAMKSEGGFIWACKNYDGDVQSDS VAQGYGSLGMMTSVLVCPDGKTVEAESAHGTVTRHYRMYQKGQETSTNPIASIFAWTRGLAHRAKLDNNK ELAFFANALEEVSIETIEAGFMTKDLAACIKGLPNVQRSDYLNTFEFMDKLGENLKIKLAQAKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005896 |
| ORF Size | 1242 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005896.4 |
| RefSeq Size | 2339 bp |
| RefSeq ORF | 1245 bp |
| Locus ID | 3417 |
| UniProt ID | O75874 |
| Domains | isodh |
| Protein Pathways | Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways |
| MW | 46.5 kDa |
| Gene Summary | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
Citations (7)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
ATRX regulates glial identity and the tumor microenvironment in IDH-mutant glioma
,null,
Genome Biology
,PubMed ID 34763709
[Isocitrate dehydrogenase]
|
|
Targeted rescue of cancer-associated IDH1 mutant activity using an engineered synthetic antibody
,Rizk, SS;Mukherjee, S;Koide, A;Koide, S;Kossiakoff, AA;,
Sci Rep
,PubMed ID 28373671
[Isocitrate dehydrogenase]
|
|
Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas
,null,
The Journal of Clinical Investigation
,PubMed ID 28319047
[Isocitrate dehydrogenase]
|
|
Registered report: IDH mutation impairs histone demethylation and results in a block to cell differentiation
,Richarson, AD;Scott, DA;Zagnitko, O;Aza-Blanc, P;Chang, CC;Russler-Germain, DA;Reproducibility Project: Cancer Biology, ;,
Elife
,PubMed ID 26971564
[Isocitrate dehydrogenase]
|
|
Mechanism for enhanced 5-aminolevulinic acid fluorescence in isocitrate dehydrogenase 1 mutant malignant gliomas
,null,
Oncotarget
,PubMed ID 26008980
[Isocitrate dehydrogenase]
|
|
Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
,Wen, H;Cho, HR;Yun, T;Kim, H;Park, CK;Lee, SH;Choi, SH;Park, S;,
J. Neurochem.
,PubMed ID 25251602
[Isocitrate dehydrogenase]
|
|
Cancer-associated metabolite 2-hydroxyglutarate accumulates in acute myelogenous leukemia with isocitrate dehydrogenase 1 and 2 mutations
,null,
The Journal of Experimental Medicine
,PubMed ID 20142433
[Isocitrate dehydrogenase]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210582L1 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L2 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RC210582L3 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC210582L4 | Lenti ORF clone of Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), mGFP tagged |
CNY 7,888.00 |
|
| RG210582 | IDH1 (tGFP-tagged) - Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 7,088.00 |
|
| SC116430 | IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
|
| SC322129 | IDH1 (untagged)-Human isocitrate dehydrogenase 1 (NADP+), soluble (IDH1) |
CNY 5,488.00 |
其它Isocitrate dehydrogenase产品
