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VPS35 (NM_018206) Human Tagged ORF Clone
CAT＃: RC210597
VPS35 (Myc-DDK-tagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35)
ORF Plasmid: tGFP
"NM_018206" in other vectors (6)
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CNY 8,744.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MEM3; PARK17 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTACAACACAGCAGTCCCCTCAGGATGAGCAGGAAAAGCTCTTGGATGAAGCCATACAGGCTGTGA AGGTCCAGTCATTCCAAATGAAGAGATGCCTGGACAAAAACAAGCTTATGGATGCTCTAAAACATGCTTC TAATATGCTTGGTGAACTCCGGACTTCTATGTTATCACCAAAGAGTTACTATGAACTTTATATGGCCATT TCTGATGAACTGCACTACTTGGAGGTCTACCTGACAGATGAGTTTGCTAAAGGAAGGAAAGTGGCAGATC TCTACGAACTTGTACAGTATGCTGGAAACATTATCCCAAGGCTTTACCTTTTGATCACAGTTGGAGTTGT ATATGTCAAGTCATTTCCTCAGTCCAGGAAGGATATTTTGAAAGATTTGGTAGAAATGTGCCGTGGTGTG CAACATCCCTTGAGGGGTCTGTTTCTTCGAAATTACCTTCTTCAGTGTACCAGAAATATCTTACCTGATG AAGGAGAGCCAACAGATGAAGAAACAACTGGTGACATCAGTGATTCCATGGATTTTGTACTGCTCAACTT TGCAGAAATGAACAAGCTCTGGGTGCGAATGCAGCATCAGGGACATAGCCGAGATAGAGAAAAAAGAGAA CGAGAAAGACAAGAACTGAGAATTTTAGTGGGAACAAATTTGGTGCGCCTCAGTCAGTTGGAAGGTGTAA ATGTGGAACGTTACAAACAGATTGTTTTGACTGGCATATTGGAGCAAGTTGTAAACTGTAGGGATGCTTT GGCTCAAGAATATCTCATGGAGTGTATTATTCAGGTTTTCCCTGATGAATTTCACCTCCAGACTTTGAAT CCTTTTCTTCGGGCCTGTGCTGAGTTACACCAGAATGTAAATGTGAAGAACATAATCATTGCTTTAATTG ATAGATTAGCTTTATTTGCTCACCGTGAAGATGGACCTGGAATCCCAGCGGATATTAAACTTTTTGATAT ATTTTCACAGCAGGTGGCTACAGTGATACAGTCTAGACAAGACATGCCTTCAGAGGATGTTGTATCTTTA CAAGTCTCTCTGATTAATCTTGCCATGAAATGTTACCCTGATCGTGTGGACTATGTTGATAAAGTTCTAG AAACAACAGTGGAGATATTCAATAAGCTCAACCTTGAACATATTGCTACCAGTAGTGCAGTTTCAAAGGA ACTCACCAGACTTTTGAAAATACCAGTTGACACTTACAACAATATTTTAACAGTCTTGAAATTAAAACAT TTTCACCCACTCTTTGAGTACTTTGACTACGAGTCCAGAAAGAGCATGAGTTGTTATGTGCTTAGTAATG TTCTGGATTATAACACAGAAATTGTCTCTCAAGACCAGGTGGATTCCATAATGAATTTGGTATCCACGTT GATTCAAGATCAGCCAGATCAACCTGTAGAAGACCCTGATCCAGAAGATTTTGCTGATGAGCAGAGCCTT GTGGGCCGCTTCATTCATCTGCTGCGCTCTGAGGACCCTGACCAGCAGTACTTGATTTTGAACACAGCAC GAAAACATTTTGGAGCTGGTGGAAATCAGCGGATTCGCTTCACACTGCCACCTTTGGTATTTGCAGCTTA CCAGCTGGCTTTTCGATATAAAGAGAATTCTAAAGTGGATGACAAATGGGAAAAGAAATGCCAGAAGATT TTTTCATTTGCCCACCAGACTATCAGTGCTTTGATCAAAGCAGAGCTGGCAGAATTGCCCTTAAGACTTT TTCTTCAAGGAGCACTAGCTGCTGGGGAAATTGGTTTTGAAAATCATGAGACAGTCGCATATGAATTCAT GTCCCAGGCATTTTCTCTGTATGAAGATGAAATCAGCGATTCCAAAGCACAGCTAGCTGCCATCACCTTG ATCATTGGCACTTTTGAAAGGATGAAGTGCTTCAGTGAAGAGAATCATGAACCTCTGAGGACTCAGTGTG CCCTTGCTGCATCCAAACTTCTAAAGAAACCTGATCAGGGCCGAGCTGTGAGCACCTGTGCACATCTCTT CTGGTCTGGCAGAAACACGGACAAAAATGGGGAGGAGCTTCACGGAGGCAAGAGGGTAATGGAGTGCCTA AAAAAAGCTCTAAAAATAGCAAATCAGTGCATGGACCCCTCTCTACAAGTGCAGCTTTTTATAGAAATTC TGAACAGATATATCTATTTTTATGAAAAGGAAAATGATGCGGTAACAATTCAGGTTTTAAACCAGCTTAT CCAAAAGATTCGAGAAGACCTCCCGAATCTTGAATCCAGTGAAGAAACAGAGCAGATTAACAAACATTTT CATAACACACTGGAGCATTTGCGCTTGCGGCGGGAATCACCAGAATCCGAGGGGCCAATTTATGAAGGTC TCATCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210597 protein sequence
Red=Cloning site Green=Tags(s) MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAI SDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGV QHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRE RERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLN PFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSL QVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKH FHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSL VGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKI FSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITL IIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECL KKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHF HNTLEHLRLRRESPESEGPIYEGLIL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018206 |
| ORF Size | 2388 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_018206.5 |
| RefSeq Size | 3298 bp |
| RefSeq ORF | 2391 bp |
| Locus ID | 55737 |
| UniProt ID | Q96QK1 |
| Domains | Vps35 |
| MW | 91.7 kDa |
| Gene Summary | This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210597L1 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), Myc-DDK-tagged |
CNY 11,144.00 |
|
| RC210597L2 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mGFP tagged |
CNY 11,144.00 |
|
| RC210597L3 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), Myc-DDK-tagged |
CNY 11,144.00 |
|
| RC210597L4 | Lenti ORF clone of Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35), mGFP tagged |
CNY 11,144.00 |
|
| RG210597 | VPS35 (tGFP-tagged) - Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) |
CNY 10,344.00 |
|
| SC113645 | VPS35 (untagged)-Human vacuolar protein sorting 35 homolog (S. cerevisiae) (VPS35) |
CNY 8,752.00 |
